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  • 1
    Electronic Resource
    Electronic Resource
    Continuous production of maltotetraose using immobilized Pseudomonas stutzeri amylase (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 32 (1988), S. 669-676 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A continuous production process of maltotetraose was investigated by using immobilized maltotetraose (G4)- forming amylase (1,4-α-D-glucan maltotetraohydrolase, EC3.2.1.60) from Pseudomonas stutzeri adsorbed on a macroporous hydrophobic resin. The maximum reaction rate was obtained at 55°C and the activation energy of hydrolysis by immobilized G4-forming amylase was calculated to be 8.45 kcal/mol. The maltotetraose yield was greatly influenced by the flow rate of substrate solution, its concentration, and the immobilized enzyme activity. The newly defined factor “specific space velocity” was successfully introduced to normalize the operating parameters. Using this factor, the immobilized enzyme reactor then can be simulated and the operating dynamics can be determined.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260320512
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  • 2
    Electronic Resource
    Electronic Resource
    Vinyl polymerization by metal complexes, 28.Part 27 of the series: K. Kimura, Y. Inaki, K. Takemoto, J. Macromol. Sci.-Chem., A9 (1975) 1399. Polymerization of acrylonitrile initiated by copper(II) chelates of vinylamine-vinylacetamide copolymers in dimethyl sulfoxide solution (1976)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 52 (1976), S. 129-142 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die durch Kupfer(II)-chelate von Polyvinylamin, Polyvinylacetamid und Vinylamin-Vinylacetamid-Copolymeren initiierte Polymerisation von Acrylnitril wurde in Dimethylsulfoxidlösung untersucht. Es ergab sich, daß die Kupfer(II)-chelate der Vinylamin-Vinylacetamid-Copolymeren die Polymerisation von Acrylnitril sogar in Abwesenheit von Tetrachlorkohlenstoff glatt auslösen können, während die Kupfer(II)-chelate von Polyvinylamin und Polyvinylacetamid die Polymerisation erst in Anwesenheit von Tetrachlorkohlenst off auslösen. Die Polymerisationsaktivität scheint in Beziehung mit der Stabilität dieser Copolymer-Kupfer(II)-chelate zu stehen. Aus den Ergebnissen der Polymerisation und sichtbarer Spektroskopie wurde der Auslösungsmechanismus diskutiert.
    Notes: The polymerization of acrylonitrile initiated by copper(II) chelates of polyvinylamine, polyvinylacetamide and vinylamine-vinylacetamide copolymers was studied in dimethyl sulfoxide solution. The copper(II) chelates of vinylamine-vinylacetamide copolymers were found to initiate the polymerization of acrylonitrile effectively, even in the absence of carbon tetrachloride, while those of polyvinylamine and polyvinylacetamide initiate only in the presence of carbon tetrachloride. The polymerization activity was assumed to be closely related to the stability of these copolymer-copper(II) chelates. From the results of the polymerization and the visible spectroscopy, an initiation mechanism is discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1976.050520112
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  • 3
    Electronic Resource
    Electronic Resource
    Vinyl polymerization by metal complexes, 26Part 25 of the series: Y. Inaki, M. Takahashi, Y. Kameo, K. Takemoto, J. Polym. Sci., Polym. Chem. Ed. in press. Vinyl polymerization initiated by polyvinylamine-copper(II) chelate in dimethylsulfoxide solution (1976)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 49 (1976), S. 103-114 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die durch Polyvinylamin-Kupfer(II)chelat initiierte Polymerisation von Acrylnitril und Methylmethacrylat wurde in Dimethylsulfoxidlösung in Gegenwart sowie Abwesenheit von Tetrachlorkohlenstoff untersucht. Zum Vergleich damit wurde auch ein dimeres Chelat, d. h., 1,3-Diaminopropan-Kupfer(II)chelat als Starter gewählt. Es ergab sich, daß die Startfähigkeit dieses dimeren Chelats in Dimethylsulfoxid im Gegensatz zu derjenigen in Wasser höher als die des polymeren Chelats war. Das dimere Kupferchelat konnte die Polymerisation von Acrylnitril sogar in Abwesenheit von Tetrachlorkohlenstoff auslösen.
    Notes: The polymerization of acrylonitrile and methyl methacrylate initiated by polyvinylamine-copper(II) chelate was studied in dimethylsulfoxide solution, in the presence and absence of carbon tetrachloride. For comparison, the dimeric chelate(1,3-diaminopropane-copper(II) chelate) was also chosen as an initiator. The initiation activity of the dimeric chelate was found to be higher than that of the polymeric chelate in dimethylsulfoxide solution, different from the cases in aqueous media. The dimeric chelate could initiate the polymerization of acrylonitrile effectively, even in the absence of carbon tetrachloride.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1976.050490109
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis and polymerization of 2-(p-methacryloyloxybenzoyl)ethyl 2′-(phthalimido)ethyl hydrogen phosphate (1976)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 177 (1976), S. 1973-1979 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Es werden die Darstellungen von Benzyl-2-(p-methacryloyloxybenzoyl)äthyl-2′-(phthalimido)äthyl-phosphat (3) und 2-(p-Methacryloyloxybenzoyl)äthyl-2′-(phthalimido)äthyl-hydrogen-phosphat (6) beschrieben. Die Monomeren werden charakterisiert und mit 2,2′-Azoisobutyronitril (AIBN) polymerisiert. Die Eigenschaften der erhaltenen Polymeren werden beschrieben.
    Notes: Benzyl 2-(p-methacryloyloxybenzoyl)ethyl 2′-(phthalimido)ethyl phosphate (3) and 2-(p-methacryloyloxybenzoyl)ethyl 2′-(phthalimido)ethyl hydrogen phosphate (6) were prepared, characterized, and polymerized with 2,2′-azoisobutyronitrile (AIBN). The properties of the resultant polymers are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1976.021770705
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  • 5
    Electronic Resource
    Electronic Resource
    Synthesis of polymers containing phosphoric ester (1976)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 177 (1976), S. 1235-1241 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Benzyl-2-(methacryloyloxy)äthyl-2′-(phthalimido)äthylphosphat (1) und 2-(Methacryloyloxy)äthyl-2′-(phthalimido)äthyl-hydrogenphosphat (3) wurden dargestellt, charakterisiert und mit 2,2′-Azoisobutyronitril polymerisiert. Die Eigenschaften der erhaltenen Polymere werden beschrieben.
    Notes: Benzyl 2-(methacryloyloxy)ethyl 2′-(phthalimido)ethyl phosphate (1) and 2-(methacryloyloxy)ethyl 2′-(phthalimido)ethyl hydrogen phosphate (3) were synthesized, characterized, and polymerized with 2,2′-azoisobutyronitrile. The properties of the resultant polymers are described.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1976.021770501
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 136 (1988), S. 421-430 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We compared proliferation and survival of various syngeneic transformed cell lines under conditions of depletion of 15 amino acids in Dulbecco-Eagle's medium. We used a normal fibroblast line 3Y1 and 22 transformed sublines of 3Y1 which had been induced by one of seven transforming agents-simian virus 40, mouse polyomavirus, adenovirus type 12, E1A gene of adenovirus type 12, cDNA of Harvey murine sarcoma virus, Rous sarcoma virus, or N-methyl-N′-nitro-N-nitrosoguanidine. Unlike other untransformed cells examined (mouse BALB/c-3T3 line, mouse NlH-3T3 line, and primary Fischer rat embryo fibroblasts), 3Y1 ceased to proliferate and accumulated in a viable state with a G1-phase DNA content under 14 singular deprivations of amino acid. None of the transformed 3Y1 lines completely arrested in the G1 phase of the cell cycle and each showed different levels of survival, depending on each transforming agent. As for transformed 3Y1 cells induced by a given virus or a given transforming gene, any one of the three sublines shared the same trend with respect to proliferation and survival. Transformed derivatives induced by N-methyl-N′-nitro-N-nitrosoguanidine showed almost the same trend in proliferation, but the patterns of survival were not uniform. Our observations suggest that the unique responses of 3Y1 to amino acid depletion are differently modified by different transforming agents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041360305
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation and visualization of Met-72-positive, metastatic variants present in B16 melanoma tumor masses (1988)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 36 (1988), S. 311-322 
    Details
    ISSN: 0730-2312
    Keywords: B16 melanoma ; metastatic variants ; met 72/83 antigen ; immunohistochemistry ; localization in situ ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Metastatic variants of the B16 melanoma displaying high experimental metastatic potential have been shown to express high levels of a 72,000-dalton glycoprotein (Met-72) on their cell surface (Kimura AK, Xiang J: J Nat Can Inst 76:1247-1253, 1986). Monoclonal antibodies (MoAb) directed against the Met-72 determinant have been used in this study as immunohistochemical reagents on preparations of fresh B16 melanoma tumors and their metastases. These immunohistochemical analyses have utilized frozen sections, impression smears, and cytospin preparations of fresh tumors harvested at various time points during tumor growth, to view the presence and location of Met-72-positive metastatic variants within tumor masses. Biotinylated anti-Met-72 MoAbs were reacted with freshly dissociated tumor cells from a B16 melanoma ovarian metastasis. These cells were then reacted with fluorescein isothiocyanate (FITC)-streptavidin and analyzed by flow cytometry. A discrete population of positively staining cells was detected and isolated by cell sorting techniques. Met-72-positivc cells were then cloned and reanalyzed after several weeks of in vitro expansion and found to have high experimental metastatic potential to ovaries. Frozen sections of subcutaneous tumors and their metastases were analyzed by immunoperoxidase techniques. A consistent finding in these studies has been that the few tumor cells which showed high intensity of Met-72 staining were positioned perivascularly and at the invading front of B16 melanoma tumors.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240360311
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  • 8
    Electronic Resource
    Electronic Resource
    Application of the higher order finite-element method to one-dimensional Schrödinger equation (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 9 (1988), S. 827-835 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The accuracy and applicability of the finite-element method of the higher order interpolation functions to the one-dimensional Schrödinger equation were examined. When the fifth-order Lagrange and Hermite interpolation functions were used as the basis functions, practically exact solutions were obtained for all eigenvalues of several model potential energy functions. It was demonstrated that the appropriate analytical integration over the potential energy function within each element is important in the matrix element evaluation. The accuracy of the method was examined for the potential functions with a double minimum, which has a large classically forbidden region. The method was also applied to evaluate the Franck-Condon factors of the transitions between the 1 1Σg+ and 2 1Σu+ states of Na2; the latter state having a double minimum in its potential energy function.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540090805
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  • 9
    Electronic Resource
    Electronic Resource
    Preparation and formation mechanism of poly(oxycarbonyl-1,4-phenylene) whiskers from 4-acetoxybenzoic acid (1988)
    Basel : Wiley-Blackwell
    Die Makromolekulare Chemie, Rapid Communications 9 (1988), S. 687-691 
    Details
    ISSN: 0173-2803
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1988.030091003
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  • 10
    Electronic Resource
    Electronic Resource
    Kinetic mechanism on Thermal Degradation of a Nitrate Ester Propellant (1988)
    Weinheim : Wiley-Blackwell
    Propellants, Explosives, Pyrotechnics 13 (1988), S. 8-12 
    Details
    ISSN: 0721-3115
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The thermal degradation of a double-base propellant has been studied to elucidate the rate-determining steps and the kinetic mechanism in a wide range of temperatures (60°C-200°C) by using modified Taliani test, thermogravimetry (TG), and temperature-varied Abel (TVA) test. The results indicate that the degradation process consists of two major reactions, homolysis and autocatalysis, depending on temperature and total pressure due to evolved gases. The activation energy for the homolysis was obtained to be 35-37 kcal/mol from Taliani and TVA tests, which fall in the range of the bond dissociation energy of the weakest bonds RO-NO2. The activation energy for the autocatalysis was determined to be 46-49 kcal/mol from Taliani and TG methods. Those values observed for the two key reactions are totally opposite to reported values in the earlier literature. The temperature dependence of the reaction rates obtained in this study implies that the homolysis is the rate-determining step in the lower temperature range, the autocatalysis in the higher temperature range.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prep.19880130103
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