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  • 11
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    The Unpublished Data from Roosevelt's Commission on Country Life (1976)
    Chicago : Periodicals Archive Online (PAO)
    Agricultural History. 50:4 (1976:Oct.) 583 
    Details
    ISSN: 0002-1482
    Topics: History , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:3013-1976-050-04-000002
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  • 12
    Electronic Resource
    Electronic Resource
    Genetics of the cell surface: Assignment of genes coding for external membrane proteins to human chromosomes 10 and 14 (1979)
    Springer
    Somatic cell and molecular genetics 5 (1979), S. 281-301 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using somatic cell hybridization gene mapping methodology, genes coding for human cell-surface proteins have been assigned to specific chromosomes. Lactoperoxidase-catalyzed iodination was employed to label external membrane proteins in cell hybrids between mouse and human cultured cells. Mouse and human external membrane proteins were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis. After electrophoresis, external membrane proteins were identified by autoradiography. An external membrane protein of 130,000 molecular weight (EMP-130) segregated concordantly with glutamic oxaloacetic transaminases (GOTs, EC 2.6.1.1), an enzyme marker encoded on chromosome 10. External membrane proteins of 195,000 and 175,000 molecular weight (EMP-195 and EMP-175) segregated concordantly with nucleoside phosphorylase (NP, EC 2.4.2.1), an enzyme marker encoded on chromosome 14. Limited proteolysis of the 195,000 and 175,000 molecular weight proteins suggests that these two proteins are modified forms of each other and are encoded by the same locus. These findings demonstrate the mapping of human genes coding for external proteins EMP-130 and EMP-195 to chromosomes 10 and 14, respectively. Chromosome analyses of cell hybrids supported these assignments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538843
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  • 13
    Electronic Resource
    Electronic Resource
    Polyethylene glycol-induced mammalian cell hybridization: Effect of polyethylene glycol molecular weight and concentration (1976)
    Springer
    Somatic cell and molecular genetics 2 (1976), S. 271-280 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400–6000 was found to occur in the concentration range of 50–55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538965
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  • 14
    Electronic Resource
    Electronic Resource
    Bioautographic visualization of aminoacylase-1: Assignment of the structural geneACY-1 to chromosome 3 in man (1979)
    Springer
    Somatic cell and molecular genetics 5 (1979), S. 11-21 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A bioautographic assay was developed for the visualization of aminoacylase-1 (N -acylamino acid aminohydrolase, ACY-1; EC 3.5.1.14) after zone electrophoresis. Bioautography and species differences in electrophoretic mobility of ACY-1 made it possible to investigate the chromosome assignment of the gene for human ACY-1 using human—mouse somatic cell hybrids. Human ACY-1 segregated concordantly with β-galactosidase-A (βGAL A;EC 3.2.1.23) but showed discordant segregation with 32 other markers representing 23 linkage groups. The β GALA gene has been previously assigned to chromosome 3. From this evidence and confirming chromosome analyses, ACY-1has been assigned to chromosome 3. A genetic polymorphism in the electrophoretic mobility of ACY was observed in mouse strains, demonstrating that this enzyme can be mapped in genetic crosses of Mus musculus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538782
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  • 15
    Electronic Resource
    Electronic Resource
    GM1-gangliosidosis: Chromosome 3 assignment of theβ-galactosidase-A gene (β GAL A ) (1979)
    Springer
    Somatic cell and molecular genetics 5 (1979), S. 147-158 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The structural gene (βGALA) coding for lysosomal β-galactosidase- A (EC 3.2.1.23) has been assigned to human chromosome 3 using man-mouse somatic cell hybrids. Human β-galactosidase-A was identified in cell hybrids with a species-specific antiserum to human liver β-galactosidase-A. The antiserum precipitates β-galactosidase-A from human tissues, cultured cells, and cell hybrids, and recognizes cross-reacting material from a patient with GM1 gangliosidosis. We have analyzed 90 primary man-mouse hybrids derived from 12 separate fusion experiments utilizing cells from 9 individuals. Enzyme segregation analysis excluded all chromosomes for βGALA assignment except chromosome 3. Concordant segregation of chromosomes and enzymes in 16 cell hybrids demonstrated assignment of βGALA to chromosome 3; all other chromosomes were excluded. The evidence suggests that GM1 gangliosidosis is a consequence of mutation at this βGALA locus on chromosome 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01539157
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  • 16
    Electronic Resource
    Electronic Resource
    Complementation of genetic disease: A velocity sedimentation procedure for the enrichment of heterokaryons (1979)
    Springer
    Somatic cell and molecular genetics 5 (1979), S. 1013-1029 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Methodology is described to enrich for heterokaryons after mammalian cell fusion. A heterogeneous cell mixture can be separated on a Sta-Put apparatus into fractions of uniform size cells by sedimentation through a 1% bovine serum albumin-5% Ficoll gradient. Unfused RAG and LM/TK− cells, differing by 10% in diameter, have been sorted by size; following fusion, larger and faster sedimenting cells were shown to be hybrids. This methodology can be utilized in genetic complementation studies of human genetic diseases where selection procedures for proliferating hybrids do not exist. When fibroblasts from individuals with Tay-Sachs disease [deficient in hexosaminidase A (HEX A−)] and Sandhoff-Jatzkewitz disease (HEX A− and HEX B−) are fused, HEX A is generated, demonstrating complementation of two different mutations. After Sta-Put fractionation, the HEX A complementation product was associated with the faster sedimenting multinuclear cells and not with the mononuclear parental cells. This methodology will facilitate detection of genetic differences in fibroblasts from related inherited disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01542657
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  • 17
    Electronic Resource
    Electronic Resource
    An X-ray microanalysis survey of the concentration of elements in the cytoplasm of different mammalian cell types (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 101 (1979), S. 493-501 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Electron probe energy dispersive X-ray microanalysis was performed on freeze-dried tissue sections. The dry weight concentration of elements (mmole/kg dry weight) was measured in the cytoplasm of several cell types from adult mice and rats. This comparative investigation showed: (1) That the energy dispersive X-ray spectrum of element concentration from the cytoplasm of a specific cell type allows one to distinguish this specific cell type from other cell types with considerable accuracy. (2) That there is a relationship between the concentration of the various elements and the ultrastructural features of the cytoplasmic regions being analyzed. For example, areas rich in ribosomes are also rich in P, K and Mg. (3) These data support the idea that K is directly involved in the control of protein synthesis. The catalog of element concentrations in the cytoplasm of 13 cell types from both mice and rats should be of value to others who seek to answer various questions about these cell types.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041010315
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  • 18
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Temperaturabhängigkeit der 31P-chemischen Verschiebung in Trialkoxyphosphazen-N-phosphoryldialkylesternProfessor Hans-Albert Lehmann zum 60. Geburtstage am 1. Mai 1979 gewidmet (1979)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 451 (1979), S. 5-11 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Temperature Dependence Investigations of 31P Chemical Shifts of Trialkoxyphosphazene N-phosphoryldialkylestersThe 31P NMR spectra of a series of trialkoxyphosphazene-N-phosphoryldialkylesters, (RO)3P=N—P(O)(OR′)2, where R = R′ = Et; R = Et, R′ = Bu; R = Bu, R′ = Et; R = R′ = Bu; and R = R = R′ = Hex respectively, and of trichlorophosphazene-N-phosphoryldichloride were investigated at various temperatures. The temperature dependence of chemical shift of phosphorus belonging to the phosphazene group is multiplicately larger than that of phosphorus belonging to the phosphoryl group. This fact also could be confirmed for other simple phosphazene and phosphoryl compounds. As discussed this different temperature dependence of 31P chemical shift is not a consequence of the medium but of the molecular structure.
    Notes: Für eine Reihe von Trialkoxyphosphazen-N-phosphoryldialkylestern, (PO)3P=N—P(O)(OR′)2, wobei R = R′ = Et; R = Et, R′ = Bu; R = Bu, R′ = Et; R = R′ = Bu bzw. R = R′ = Hex waren, sowie für Trichlorphosphazen-N-phosphoryldichlorid wurden jeweils 31P-NMR-Spektren bei verschiedenen Temperaturen aufgenommen. Es zeigte sich, daß die Temperaturabhängigkeit der chemischen Verschiebung für den Phosphor der Phosphazen-Gruppierung um ein mehrfaches größer ist als die für den Phosphor der Phosphoryl-Gruppierung. Dies konnte auch allgemein für andere einfache Phosphazen- und Phosphoryl-Verbindungen bestätigt werden. Es wird diskutiert. daß diese unterschiedliche Temperaturabhängigkeit der 31P-chemischen Verschiebung nicht auf Medieneinflüsse, sondern auf die molekulare Struktur zurückzuführen ist.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19794510102
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  • 19
    Electronic Resource
    Electronic Resource
    NMR-spektroskopische Untersuchungen an Mercaptophosphazenen. II. NMR-spektroskopische Untersuchungen an 15N-markierten Mercaptophosphazenen (1979)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 448 (1979), S. 107-114 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: N.M.R. Spectroscopic Investigations of Thiophosphazenes. II. N.M.R. Spectroscopic Investigations of 15N Labelled ThiophosphazenesCompletely 15N labelled compounds of the type 15N3P3Cl6-n(SR)n, R = Et or Ph; n = 0, 2, 4, or 6, and of the type 15N4P4Cl8-n(SR)n, R = Et; n = 0, 4, or 8, were prepared and investigated by means of both 31P n.m.r. spectroscopy and 15N n.m.r. spectroscopy respectively. The coupling constants 2JPP, in some cases only found by simulating the spectra, and the coupling constants 1JPN are given. The values of these coupling constants and their relation are discussed. The general tendency is visible, that with increasing coupling constant 1JPN the coupling constant 2JPP decreases. With increasing grade of substitution n the 15N chemical shifts are changed to higher fields.
    Notes: Vollständig 15N-markierte Verbindungen des Typs 15N3P3Cl6-n(SR)n, R = Et, Ph; n = 0, 2, 4, 6, und des Typs 15N4P4Cl8-n(SR)n, R = Et; n = 0, 4, 8, wurden synthetisiert und mit Hilfe der 31P-NMR-Spektroskopie und der 15N-NMR-Spektroskopie untersucht. Die Kopplungskonstanten 2Jpp, die in einigen Fällen nur auf dem Wege der Spektrensimulation gefunden werden, sowie die Kopplungskonstanten 1JPN werden angegeben und ihre jeweilige Änderung und ihr Verhältnis zueinander diskutiert. Es wird die allgemeine Tendenz sichtbar, daß bei zunehmender Kopplungskonstante 1JPN die Kopplungskonstante 2JPP abnimmt. Mit zunehmendem Substitutions-grad n ändern sich die 15N-chemischen Verschiebungen nach höherem Feld.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19794480112
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  • 20
    Electronic Resource
    Electronic Resource
    NMR-spektroskopische Untersuchungen an Mercaptophosphazenen. I. Verlauf der Reaktion von Natriumethylmercaptid mit Hexachlorocyclotriphosphazatrien bzw. Octachlorocyclotetraphosphazatetraen (1979)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 448 (1979), S. 100-106 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: N.M.R. Spectroscopic Investigations of Thiophosphazenes. I. Reactions of Sodium Ethylthiolate with Hexachlorocyclotriphosphazatriene and OctachlorocyclotetraphosphazatetraeneBy reactions of hexachlorocyclotriphosphazatriene with an excess of sodium ethylthiolate as a suspension in ether all of the possible geminal derivatives N3P3Cl6-n(SEt)n; n = 1-6, are formed in different quantities. Products from reactions of octachlorocyclotetraphosphazatetraene with sodium ethylthiolate, N4P4Cl8-n(SEt)n, likewise contain all of the geminally substituted derivatives up to n = 8 with the exception of the derivatives with n = 1 and n = 7. Compounds with n = 3, 4, or 5 exist in two geminal isomers respectively. Nongeminal ethylthiochlorophosphazenes were not found by any reaction. The formed compounds were isolated by chromatographic methods and studied by 31P n.m.r. spectroscopy.
    Notes: Bei Umsetzungen von Hexachlorocyclotriphosphazatrien mit einem Überschuß an Natriumethylmercaptid in etherischer Suspension entstehen in unterschiedlichen Mengen alle denkbaren geminalen Substitutionsprodukte N3P3Cl6-n(SEt)n; n = 1-6. Im Reaktionsprodukt des Octachlorocyclotetraphosphazatetraens mit Natriumethylmercaptid, N4P4Cl8-n(SEt)n, lassen sich außer den Verbindungen mit n = 1 und n = 7 ebenfalls alle geminalen Substitutionsprodukte biz zu n = 8 nachweisen, wobei sich für die Verbindungen mit n = 3, 4, 5 jeweils beide geminalen Isomeren bilden. Nichtgeminale Mercaptochlorophosphazene waren bei keiner Umsetzung nachweisbar. Die entstandenen Verbindungen wurden säulenchromatographisch isoliert und 31P-NMR-spektroskopisch untersucht.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19794480111
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