ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Various types of cells from the testes of mice and hamsters were separated according to differences in sedimentation velocity by centrifugal elutriation, a counterflow centrifugation technique. Approximately 3 × 108 cells, prepared from six mouse testes or from one hamster testis, were separated into 11 fractions in less than two hours as compared to the 4-5 hours required for sedimentation at unit gravity (“Staput”). Fractions enriched in elongated spermatids and spermatozoa (100%), stages 1-8 spermatids (69%) and pachytene spermatocytes (58%) were obtained from mouse testis dispersions. Similarly enriched fractions were obtained from hamster cells. A single fraction enriched in stages 1-8 spermatids (mouse) was prepared in less than 30 minutes. As many as 2 × 109 cells were separated in a single procedure. Spermatogenic cells exhibited no evidence of structural damage with trypan blue and phase microscopy, and recovery was essentially 100%. Centrifugal elutriation had no effect on sperm motility or on the plating efficiency of CHO cells.
Additional Material:
4 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040860119
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