ISSN:
1744-313X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Monolayers of mouse peritoneal macrophages actively incorporated 14C-labelled uridine, an RNA precursor. This uptake did not seem to be due to in vitro activation or stimulation of the cells but rather to their high RNA metabolic activity. 14C-uridine uptake by the unstimulated adherent cells was a characteristic property of macrophages, because it was not reduced when the cells were (i) pretreated with trypsin (to detach remaining lymphocytes), (ii) precultured for several days (to allow PMN to die off) and (iii) pretreated with anti-mouse immunoglobulin or anti-θ serum plus complement.Pretreatment of macrophage monolayers with antibody plus complement, followed by a measurement of their 14C-uridine uptake could be used to test for anti-macrophage activity, for instance in certain anti-B cell or anti-T cell sera, or to detect cell surface antigens on macrophages. With this technique we demonstrated the presence of H-2 and Ia alloantigens on peritoneal macrophages. The presence of Ia antigens on macrophages may be of particular biological significance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1744-313X.1975.tb00548.x
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