ISSN:
1573-4919
Keywords:
chloroplast
;
DNA
;
characterization
;
safflower
;
isolation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Summary Safflower (Carthamus tinctorius L.) chloroplasts were isolated and purified with the aid of commercially available nylon mesh, differential centrifugation, and DNase I treatment. These chloroplasts were free of nuclei as determined by light microscopy of aceto orcein stained preparations, and similar to those observed by electron microscopy in spinach and many other higher plants, being bounded by a double membrane layer and characterized by the presence of a lamellar system surrounded by embedding matrix, and stacked membranes or grana lamallae. DNA was isolated and purified from such chloroplasts, and characterized with respect to cesium chloride density gradient isopycnic centrifugaton, denaturation, renaturation kinetics and restriction enzyme analysis. These studies show that safflower chloroplast DNA is similar to many other higher plant DNAs having a density of 1.700 gl cm3(G + C = 40.8%), a Tm of 86°C (G + C = 40.7%) and a molecular complexity and genome size of about 108 daltons.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00221101
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