ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Parameter estimation studies have been conducted employing mathematical models developed previously by the investigators and experimental data collected by the last author. A batch fermentation process in which Candida lipolytica were cultured on n-hexadecane dissolved in dewaxed gas oil was employed to obtain the experimental data. The kinetic data from a number of batch experiments conducted at different initial substrate concentrations and different dispersed phase volume fractions were analyzed assuming that, the basic model parameters (maximum specific growth rate, saturation constant, substrate phase equilibrium constant, adsorption constant, desorption constant, etc.) did not change from experiment to experiment. The Gauss-Newton method with modification by Greenstadt, Eisenpress, Bard, and Carroll was used to minimize the conventional sum of squares criterion on the IBM 300/50 computer. The individual confidence intervals were obtained for each individual parameter. Tin- models were compared employing the F-test for equality of variances and an analysis of residuals. For the two best models, the estimated parameter values were compared with available experimental information. The results showed good agreement between the experimental data and the values predicted by the mathematical models. The results presented in this work did suggest that growth on small segregated drops may be more important than continuous phase growth on dissolved substrate.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260140403
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