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  • Cell & Developmental Biology
  • 1980-1984  (33)
  • 1975-1979
  • 1970-1974  (17)
  • 1960-1964
  • 1950-1954
  • 1980  (33)
  • 1971  (17)
Collection
Keywords
Publisher
Years
  • 1980-1984  (33)
  • 1975-1979
  • 1970-1974  (17)
  • 1960-1964
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    Meeting report (1980)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1980), S. 159-162 
    Details
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970010112
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  • 2
    Electronic Resource
    Electronic Resource
    Physarum myosin light chain binds calcium (1980)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1980), S. 63-71 
    Details
    ISSN: 0886-1544
    Keywords: Physarum polycephalum ; myosin light chains ; polyacrylamide gel electrophoresis ; calcium ; cytoplasmic streaming ; actomyosin ATPase regulation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Myosin from the slime mold Physarum polycephalum contains three sizes of polypeptides: a heavy chain and two light chains, LC-1 and LC-2. Using a simple qualitative test for calcium binding by comparing electrophoretic migration of the polypeptides in sodium dodecy1 sulfate (SDS) acrylamide gels in the presence and absence of calcium, we have found that Physarum myosin light chain LC-2 migrates with an apparent molecular weight of 16,900 daltons in the presence of the metal ion chelator ethylene glycol bis (B-aminoethyl ether) N,N′-tetraacetic acid (EGTA). However, if calcium chloride is added to the sample prior to electrophoresis, the apparent molecular weight decreases to 16,100. Lanthanide and cadmium ions, but not magnesium, can substitute for calcium. Because the ionic radii of Ca2+, La3+, and Cd2+ are almost identical, we conclude that Physarum myosin LC-2 possesses a very size-specific binding site for calcium. Physarum myosin LC-1 and the heavy chain give no evidence for binding calcium by this test. Since cytoplasmic streaming in the plasmodium of Physarum requires calcium, our evidence indicates that the calcium-binding property of Physarum myosin LC-2 may be important in regulating the production of force by actomyosin in the ectoplasm. Unexpectedly, the myosin light chain in Physarum capable of binding calcium, LC-2, is the essential light chain, while LC-1 is a member of the regulatory class of myosin light chains [V. T. Nachmias, personal communication]. Until now, essential myosin light chains have not been shown to have high affinity divalent cation binding sites. This means a new version of the myosin-based model for actomyosin regulation by calcium may be required to explain cytoplasmic movement in Physarum, and perhaps in other motile systems involving cytoplasmic myosins as well.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970010106
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  • 3
    Electronic Resource
    Electronic Resource
    RNA metabolism in thoracic duct lymphocytes from thymectomized ratsSupported by grant IC-18A from the American Cancer Society, grant AI-08642 from the National Institute of Allergy and Infectious Diseases. (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 78 (1971), S. 465-467 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040780316
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  • 4
    Electronic Resource
    Electronic Resource
    Vital DNA staining and cell sorting by flow microfluorometry (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 102 (1980), S. 175-181 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4′6-diamidino-2-pheylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is a suitable vital stain for a varietyof cell types. Hoechst 33258 and DAPI, however, are quantitative vital stains for CHO cells only. Cloning efficiency is unaffected by the sorting procedure, and these stains are not mutagenic at concentrations suitable for vital staining. Potential applications of this procedure to cell biology are discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041020208
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of lymphoid leukemia colony-forming cells in abelson virus infected mice: Differences in inbred strains (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 104 (1980), S. 153-162 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: BALB/c or DBA/2 mice were infected with Abelson murine leukemia virus (A-MuLV), pseudotype Molony murine leukemia virus (M-MuLV). Infection of these mice with 104 focus-forming units of A-MuLV (M-MuLV) induced overt leukemia, detectable grossly or microscopically in 90% of the mice at 20-38 days. However, these methods did not detect leukemia at 17 days or before. Bone marrow cells from A-MuLV-infected leukemic or preleukemic mice were placed in tissue culture in a soft agarose gel. Cells from leukemic or preleukemic BALB/c mice grew to form colonies of 103 cells or more, composed of lymphoblasts, whereas marrow cells from normal uninfected mice did not. Cells from these colonies grew to form ascitic tumors after intraperitoneal inoculation into pristane-primed BALB/c recipient. Colony-forming leukemia cells could be detected in the marrow of A-MuLV-infected mice as early as 8 days after virus incoluation. The number of colony-forming leukemia cells increased as a function of time after virus inoculation.Colony-forming leukemia cells require other cells in order to replicate in tissue culture. Normal bone marrow cells, untreated or after treatment with mitomycin-C, provide this “helper” function. Only in the presence of untreated or mitomycin-C treated helper cells was the number of colonies approximately proportional to the number of leukemia cells plated.Marrow cells from leukemic BALB/c mice form more colonies than those from leukemic DBA/2 mice. The number of colonies formed per 103 microscopically identifiable leukemia cells plated was determined to be 2-3 for leukemic BALB/c mice and 0.3 for DBA/2 mice. Cocultivation of leukemic DBA/2 marrow cells with mitomycin-C treated normal BALB/c cells did not increase the number of colonies formed by the DBA/2 leukemic cells. Thus, the decreased ability of DBA/2 leukemia cells to form colonies appears to be a property of the leukemia cell population.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041040204
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of ouabain on growth regulation by serum components in Balb/C-3T3 cells: Inhibition of entry into s phase by decreased protein synthesis (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 105 (1980), S. 439-448 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of inhibition of the cell membrane Na+-K+ pump on the Balb/c-3T3 cell growth cycle was studied. Inhibition of the Na+-K+ pump resulted in a dose-dependent reduction of intracellular K+ concentration ({K+}i). However, inhibition of protein synthesis in G0/G1 and of subsequent entry into S phase occurred only after {K+}i fell below a critical threshold (50-60 mmoles/liter). Thus, when the {K+}i falls below a critical threshold, protein synthesis is inhibited, preventing cells from entering the S phase.The platelet-derived growth factor (PDGF) induces cells to become “competent” to traverse the cell cycle; the platelet-poor plasma component of serum allows competent cells to progress through G0/G1 and enter S phase. Inhibition of the Na+-K+ pump did not prevent the induction of competence by PDGF, but it did reversibly inhibit plasma-mediated events in early G0/G1. Similarly, cycloheximide inhibited plasma-mediated events but did not prevent PDGF-induced competence. Thus, protein synthesis may not be required for induction of competence; alternatively, the induction of the competent state may occur in these cells after removal of PDGF and protein synthesis inhibitor. Protein synthesis is required for subsequent plasma-mediated events in G0/G1.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041050308
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  • 7
    Electronic Resource
    Electronic Resource
    The fine structure of the pigment epithelium and photoreceptor cells of the newt, Triturus viridescens dorsalis (Rafinesque)This work was supported by grants from the Medical Research Council of Canada, the E. A. Baker Foundation for the Prevention of Blindness and Fight For Sight, Inc. (1971)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 135 (1971), S. 389-431 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology of the retinal pigment epithelium and photoreceptor cells has been studied in the common newt Triturus viridescens dorsalis by light, conventional transmission and scanning electron microscopy.The pigment epithelium is formed by a single layer of low rectangular cells, separated by a multilayered membrane (Bruch's membrane) from the vessels of the choriocapillaris. The scleral border of the pigment epithelium is highly infolded and each epithelial cell contains smooth endoplasmic reticulum, myeloid bodies, mitochondria, lysosomes, phagosomes and an oval nucleus. Inner, pigment laden, epithelial processes surround the photoreceptor outer and inner segments.The three retinal photoreceptor types, rods, single cones and double cones, differ in both external and internal appearance. The newt, rod, outer segments appear denser than the cones in both light and electron micrographs, due to a greater number of rod lamellae per unit distance of outer segment and to the presence of electron dense intralamellar bands. The rod outer segments possess deep incisures in the lamellae while the cone lamellae lack incisures. Both rod and cone outer segments are supported by a peripheral array of dendritic processes containing longitudinal filaments which originate in the inner segment. The inner segment mitochondria, forming the rod ellipsoid, arelong and narrow while those in the cone are spherical to oval in shape. The inner segments of all three receptor cell types also contain a glycogen-filled paraboloid and a myoid region, just outside the nucleus, rich in both rough and smooth endoplasmic reticulum. The elongate, cylindrical nuclei differ in density. The rod nuclei are denser than those of the cones, contain clumped chromatin and usually extend further vitreally. Similarly, the cytoplasm of the rod synaptic terminal is denser than its cone counterpart and contains synaptic vesicles almost twice as large as those of the cones. Photoreceptor synapses in rods and cones are established by both superficial and invaginated contacts with bipolar or horizontal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051350402
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  • 8
    Electronic Resource
    Electronic Resource
    A light and electron microscopic study of the heart of a crayfish, Procambarus clarkii (Giraud). II. Fine structureSupported by N.I.H. grant 5T1-GM-793 and by research grants HE-6100 and HE-2490 from the United States Public Health Service. (1971)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 133 (1971), S. 353-373 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The adventitia of the crayfish heart is composed of cells that are separated from each other by an intercellular space about 280 Å wide. Desmosomes are present on apposing surfaces of adjacent cells. A basal lamina underlies the adventitia and consists of a dense, amorphous substance that contains numerous fine filaments.The myocardial cells are striated and an external lamina 0.1 μ thick is present on the surface of the plasma membrane. The nuclei and most of the cytoplasm, glycogen and mitochondria are located at the cell periphery. The myofibrils are composed of thick and thin filaments and confined to the core of the cell. A T system and a well-developed SR are present. Elements of these organelles form dyads at levels that correspond to the H bands, and triads at levels that correspond to the Z bands of the peripheral myofibrils. The relationship of the T tubules to the myofibrils is discussed.Locus cells exhibit a unique pattern of intracellular myofibrillar branching. They branch from a region which has a structure similar to the Z band material. The myofibrils radiate outwardly in various directions and form numerous cellular branches which form intercalated discs with adjacent myocardial cells. These discs are more complex than those observed in poikilothermic vertebrates but are simpler than those in mammals.An endocardium is lacking in the crayfish heart but interstitial cells are present in close association with the myocardial cells and neural elements. Terminal nerve processes deeply embedded in the myocardial cells are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051330308
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  • 9
    Electronic Resource
    Electronic Resource
    Structure and sensory apparatus of oral remnants of the nasopalatine canals in the fin whale (Balaenoptera physalus L.)This investigation was supported in part by a research contract with the Fisheries Research Board of Canada. (1971)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 134 (1971), S. 271-279 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Light microscopy of serially sectioned nasopalatine duct remnants in ventral rostral integument of four adult (2 ♂, 2 ♀) fin whales reveals: (1) a common structure in all, (2) blindly ending nasopalatine pits 4 to 9 mm deep, (3) solid epithelial duct remnants 12 to 15 mm long, (4) lack of chemoreceptor endings, and (5) an abundance of presumed mechanoreceptors, mostly of the Pacinian category on the adoral sides, but also including some thinly encapsulated and perivascular ones that extend into the abundant connective tissue papillae of the duct remnants. Comparative and evolutionary relations of these structures are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051340303
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  • 10
    Electronic Resource
    Electronic Resource
    Quantitative histology of changes with age in rat bone cortexSupported in part by the National Institutes of Health Training grant GM 445. (1971)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 133 (1971), S. 241-251 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Changes with age in bone cortex of the rat were investigated by establishing histological parameters which could be quantitated to estimate age at death. Decalcified cross sections of mandible, femur and tibia were prepared from rats two to 120 days old, and measurements were made of: (1) number of osteons, (2) average number of lamellae per osteon, (3) average Haversian canal diameter, and (4) number of non-Haversian (primary) canals. Multiple regression techniques were used to estimate age at death from several combinations of these variables.With age, the number of osteons per unit area of bone and the number of lamellae per osteon increased, but Haversian canal diameter and the number of primary canals decreased. Multiple regression analyses indicated that age at death could be estimated to ± 3 days of the true value in 95% of the cases. Nomographs based on histological measurements of each bone were prepared which can provide accurate estimates of age between two and 120 days in the Sprague-Dawley female rat. It was concluded that microstructure of bone cortex can not only be quantitated to provide accurate estimates of age but it may also constitute a sensitive measure of the metabolic state of the organism. The techniques utilized should prove useful in anthropology as well in studies of bone aging.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051330208
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