ALBERT

Notes: Zusammenfassung. Es wurde die DNS- und RNS-Biosynthese von Neurospora crassa Wildstamm 3a6A unter Einfluss unterschiedlicher Konzentrationen von 2,4-D, Amitrol, Atrazin, Chlorpropham und Chlorflurenol untersucht. Die Variation der Herbizide erfolgte von 10−3 bis 10−6 M. Der in beliifteten Kulturen gezüchtete Pilz ist gegenuber Chlorpropham am empfindlichsten. Bei 10−3 M ist die Toxizitätsgrenze überschritten und das Wachstum vollständig gehemmt. 2,4-D hat in einer Konzentration von 10−3 M den grössten Einfluss auf die Nucleinsäure-Biosynthese. Die DNS-Werte erhöhen sich gegenüber der Kontrolle aiif 140%. Unter Einfluss von Atrazin (10−3 M) scheint besonders die Protein-Synthese stimuliert zu werden. Die Zunahme beträgt hier fast 140%. Allgemein haben die untersuchten 5 Herbizide einen relativ geringen Einfluss auf die Nucleinsäure-Biosynthese. Ein grösserer Effect ist nur bei der höchsten Herbizid-Konzentration von 10−3 M ZU erkennen. Der Pilz ist gegenüber diesen Herbiziden wenig empfindlich. Effets des herbicides 2,4-D, aminotriazole, atrazine, chlorprophame et chlorflurénol sur la biosynthése de l'acide nucléique chez un ascomycéte; Neurospora crassaRésumé. La biosynthése de l'ADN et de l'ARN fut déterminée chez la race sauvage 3a6A de Neurospara crassa, cultivé sur des milieux contenant du 2, 4-D, de l'aminotriazole, de l'atrazine, du chlorprophame et du chlorflurenol à des concentrations de 10−3 a 10−6 M. En culture aéréc, JV. crassa fut plus sensible au chlorprophame et à 10−3 M. sa croissance fut complétement inhibée. L'effet le ptus marqué sur la biosynthésc de I'acide nucléique fut observé avec lc 2,4-D à 10−3 M. concentration qui porta la teneur en ADN à 140% de celle du téemoin. Avec l'atrazine à 10−3 M. la synthése des proteines en particulier sembla stimulée jusqu’à atteindre environ 140% du témoin. En général, les cinq herbicides etudids n'eurent qu'un effet relativement faibie sur la biosynthése de l'acide nucléique; seule la concentration la plus forte, 10−3 M. cut un effet appreciable. N. crassa ne se montra pas tres sensible à ces herbicides. Effects of the herbicides 2,4-D, aminotriazole, atrazine, chlorpropham and chlorflurenol on nucleic-acid biosynthesis in the ascomycete Neurospora crassaSummary. Biosynthesis of DNA and RNA was determined in Neurospora crassa wild race 3a6A grown in cultures containing 2,4-D, aminotriazole, atrazine, chlorpropham and chlorflurenol in concentrations of 10−3-10−6 M. In aerated culture, N. crassa was most sensitive to chlorpropham and at 10−3 M growth was completely inhibited. The greatest effect on nucleic-acid biosynthesis was given by 2,4-D at 10−3 M which increased the DNA value to 140% of the control. With atrazine at 10−3 M. protein synthesis in particular appeared to be stimulated, to almost 140% of the control. In general, the five herbicides studied had a relatively slight effect on nucleic-acid biosynthesis; only with the highest concentration of 10−3 M was the effect appreciable. N. crassa was not very susceptible to these herbicides.

Notes: : This paper describes the techniques of factor and discriminant analyses to isolate and quantify the statistical differences between firms located on flood plains and those located off flood plains. The research effort described consists of three segments: data collection, isolation of potential classification variables, and the determination of the appropriate discriminant functions to classify a given fm as either on or off the flood plain. Significant classification functions are developed for both manufacturing and commercial establishments, whose arguments include dollar sales volume, total shipping cost, total employee cost, dollar valuations on the building and inventories, all on an annual basis, and the square footage of the site.

Notes: The fine structure of the dividing nucleus in the intracellular amastigote forms of Trypanosoma cruzi from tissue cultures has been described. In the first phase of division the nucleus shows a homogenous structure owing to the dispersion of its chromatin and nucleolar material. Microtubules similar to those of a mitotic spindle in metozoan cells then appear, running from one pole to the other. They disappear when the division of the nucleus is complete and the chromatin and the nucleolar material reorganize into their former positions.

Notes: [Auszug] Antigen T was demonstrated by immunofluorescence on fixed cells as previously described5-6. Two positive antisera were used. The counts were done on 103 cells for three independent experiments. The parental SEWA cells and the A9/ SEWA hybrid were both strongly positive. The parental A9 strain was ...

Notes: [Auszug] Angiotensin II is a polypeptide hormone which has, among many pharmacological actions, the basic property to contract smooth muscles2. The availability of a 3H-angiotensin II having a high specific activity and possessing the full biological activity of the native hormone3 allowed us to demonstrate ...