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  • Artikel  (3)
  • Weitere Quellen  (2)
  • Synthetic Biology and Assembly Cloning  (3)
  • Geol. aspects  (2)
  • Oxford University Press  (3)
  • Elsevier  (2)
  • 2020-2023
  • 2010-2014  (3)
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  • Artikel  (3)
  • Weitere Quellen  (2)
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  • 2020-2023
  • 2010-2014  (3)
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  • 1
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    Unbekannt
    The geomorphology and sedimentology of the July 9th 1956 Aegean Sea tsunami (1999)
    Elsevier
    In:  Marine Geology (Internat. J. of Marine Geol., Geochem. and Geophys.), Amsterdam, Elsevier, vol. 21, no. 1, pp. 83-96, pp. 2486, (ISBN 1-86239-117-3)
    Details
    Publikationsdatum: 1999
    Schlagwort(e): Tsunami(s) ; Geol. aspects
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    BIBLIOGRAFIE SEISMOLOGIE
  • 2
    facet.materialart.
    Unbekannt
    Composition, Deep Structure and Evolution of Continents (1999)
    Elsevier
    In:  200 pp., Elsevier, vol. 24, no. ALEX(01)-FR-77-01, AFTAC Contract F08606-76-C-0025, pp. 329, (ISBN: 0-444-50309-9)
    Details
    Publikationsdatum: 1999
    Schlagwort(e): Plate tectonics ; Textbook of geology ; Textbook of geophysics ; Earth model, also for more shallow analyses ! ; Geol. aspects ; Mineralogy
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    BIBLIOGRAFIE SEISMOLOGIE
  • 3
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    Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases (2012)
    Oxford University Press
    Details
    Publikationsdatum: 2012-04-24
    Beschreibung: Targeted gene addition to mammalian genomes is central to biotechnology, basic research and gene therapy. For example, gene targeting to the ROSA26 locus by homologous recombination in embryonic stem cells is commonly used for mouse transgenesis to achieve ubiquitous and persistent transgene expression. However, conventional methods are not readily adaptable to gene targeting in other cell types. The emerging zinc finger nuclease (ZFN) technology facilitates gene targeting in diverse species and cell types, but an optimal strategy for engineering highly active ZFNs is still unclear. We used a modular assembly approach to build ZFNs that target the ROSA26 locus. ZFN activity was dependent on the number of modules in each zinc finger array. The ZFNs were active in a variety of cell types in a time- and dose-dependent manner. The ZFNs directed gene addition to the ROSA26 locus, which enhanced the level of sustained gene expression, the uniformity of gene expression within clonal cell populations and the reproducibility of gene expression between clones. These ZFNs are a promising resource for cell engineering, mouse transgenesis and pre-clinical gene therapy studies. Furthermore, this characterization of the modular assembly method provides general insights into the implementation of the ZFN technology.
    Schlagwort(e): Synthetic Biology and Assembly Cloning
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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    AKTUELLE ARTIKEL
  • 4
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    'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules (2012)
    Oxford University Press
    Details
    Publikationsdatum: 2012-10-10
    Beschreibung: We developed a highly scalable ‘shotgun’ DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.
    Schlagwort(e): Synthetic Biology and Assembly Cloning
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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    AKTUELLE ARTIKEL
  • 5
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    A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity (2012)
    Oxford University Press
    Details
    Publikationsdatum: 2012-11-04
    Beschreibung: Recent advances have demonstrated the use of RNA-based control devices to program sophisticated cellular functions; however, the efficiency with which these devices can be quantitatively tailored has limited their broader implementation in cellular networks. Here, we developed a high-efficiency, high-throughput and quantitative two-color fluorescence-activated cell sorting-based screening strategy to support the rapid generation of ribozyme-based control devices with user-specified regulatory activities. The high-efficiency of this screening strategy enabled the isolation of a single functional sequence from a library of over 10 6 variants within two sorting cycles. We demonstrated the versatility of our approach by screening large libraries generated from randomizing individual components within the ribozyme device platform to efficiently isolate new device sequences that exhibit increased in vitro cleavage rates up to 10.5-fold and increased in vivo activation ratios up to 2-fold. We also identified a titratable window within which in vitro cleavage rates and in vivo gene-regulatory activities are correlated, supporting the importance of optimizing RNA device activity directly in the cellular environment. Our two-color fluorescence-activated cell sorting-based screen provides a generalizable strategy for quantitatively tailoring genetic control elements for broader integration within biological networks.
    Schlagwort(e): Synthetic Biology and Assembly Cloning
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    AKTUELLE ARTIKEL
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