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  • 1
    Call number: IASS 21.94624
    Type of Medium: Monograph available for loan
    Pages: XXIII, 230 S.
    ISBN: 9781853833298 , 1853833290 , 185383324X
    Language: English
    Branch Library: RIFS Library
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  • 2
    ISSN: 1572-8854
    Keywords: X-ray diffraction ; xanthenedione ; heterocyclic fused rings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Several 9-(2-R phenyl)xanthenediones have been synthesized and the x-ray diffraction structure for the 2-methylphenyl derivative (4b) has been determined. This compound crystallizes in the monoclinic system, space group P21/n, with a = 11.729 (3), b = 9.674 (3), c = 14.628 (4) Å, and β = 106.30°. It presents a partially hydrogenated xanthene system in distorted boat conformation for the heterocyclic central ring, and an almost ideal envelope conformation for the outer rings. The aromatic substituent at the ninth position is at 84° in angle with the xanthene system.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Terra nova 9 (1997), S. 0 
    ISSN: 1365-3121
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The Sierra Nevada core, located in the Betic hinterland, features a N-S large-scale open antiform with a central relatively uplifted highly extended domain placed between two less extended domains (in the east and in the west) dipping eastwards and westwards, respectively. The core-bounding detachment system formed during the Serravallian (15–11 Ma) in an episode of ENE-WSW extension. The ESCI-Beticas 2 deep seismic reflection profile, a transect through the core, shows a highly reflective deep crust overlying a subhorizontal Moho, and a fairly transparent upper crust and upper mantle. The lack of Moho relief beneath this area, with differential values for supra-crustal thinning, suggests a mechanism of intracrustal isostatic compensation. Surface geology data together with seismic imaging indicate intracrustal flow and upward doming as a response to footwall unloading accompanying the middle Miocene supracrustal extension. A prominent mid-crustal reflector (MCR) is deemed to represent a decoupling zone between the upper and the deep crust. Subsequent N-S shortening and associated folding occurred in the late Miocene. The interference pattern of this folding over the middle Miocene core produced the current E–W dome-shaped tectonic windows where the deepest complex of the Betic hinterland crops out.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 31 (1999), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A haem peroxidase different from other microbial, plant and animal peroxidases is described. The enzyme is secreted as two isoforms by dikaryotic Pleurotus eryngii in peptone-containing liquid medium. The corresponding gene, which presents 15 introns and encodes a 361-amino-acid protein with a 30-amino-acid signal peptide, was isolated as two alleles corresponding to the two isoforms. The alleles differ in three amino acid residues and in a seven nucleotide deletion affecting a single metal response element in the promoter. When compared with Phanerochaete chrysosporium peroxidases, the new enzyme appears closer to lignin peroxidase (LiP) than to Mn-dependent peroxidase (MnP) isoenzymes (58–60% and 55% identity respectively). The molecular model built using crystal structures of three fungal peroxidases as templates, also showed high structural affinity with LiP (Cα-distance 1.2 Å). However, this peroxidase includes a Mn2+ binding site formed by three acidic residues (E36, E40 and D175) near the haem internal propionate, which accounts for the ability to oxidize Mn2+. Its capability to oxidize aromatic substrates could involve interactions with aromatic residues at the edge of the haem channel. Another possibility is long-range electron transfer, e.g. from W164, which occupies the same position of LiP W171 recently reported as involved in the catalytic cycle of LiP.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The diversity, growth and symbiotic behaviour of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay were analysed. Partial sequencing of the 16S rDNA indicated that these isolates belong to Sinorhizobium meliloti species. IS-fingerprinting analysis revealed a high diversity among the isolates but some of them appear related to inoculant strains currently used in the region. The S. meliloti isolates showed a decreased growth rate with increasing acidity. They were, however, able to nodulate alfalfa at pH 5.6, but showed a delayed nodulation and decreased nodule number typical of S. meliloti strains. The impaired nodulation of S. meliloti at pH 5.6 did not result in a reduction of alfalfa dry matter production or nitrogen content. However, significant differences were observed for the relative symbiotic effectiveness of the strains analysed. LPU63 (Argentina) was the most effective among the isolates and exhibited a high nodulation competitiveness at both neutral and acidic pH. These results suggest that the isolate LPU63 may be a potential efficient inoculant for alfalfa in acid soils.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0827
    Keywords: Key words: Human osteoblastic cells — Skeletal site — Aging.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone loss with aging may be due, at least in part, to inadequate bone formation. Moreover, the process of bone aging is known to follow a different pattern throughout the skeleton. In this study, we examined the cell proliferation rate (area under the cell growth curve, AUC) and the secretion of C-terminal type I procollagen (PICP), alkaline phosphatase (ALP), and osteocalcin (OC) in primary cultures of osteoblastic cells from human trabecular bone. Osteoblastic cells were obtained for 168 donors (100 women and 68 men). Ninety-eight bone samples were obtained from subjects undergoing knee arthroplastia, 52 aged 50–70 years (64 ± 5) and 46 over age 70 (73 ± 2). Another 70 bone samples were obtained from subjects undergoing hip arthroplastia; 51 were 50–70 years old (64 ± 4) and 19 were over 70 (75 ± 5). Osteoblastic cells from the older donors had a lower proliferation rate and OC secretion than those from younger subjects. However, ALP secretion was higher in the former subjects, whereas PICP secretion was unchanged. Osteoblastic cells from hip had a lower proliferation rate than those from knee. PICP secretion was also lower and ALP secretion was higher in the former cells. In age-matched cell cultures, osteoblastic cells from the knee had higher proliferation rate and PICP secretion than osteoblastic cells from the hip. However, ALP secretion was lower in knee osteoblastic cells than those from hip only in the younger group. With aging, ALP secretion was found to increase in knee osteoblactic cells, whereas OC secretion decreased in osteoblastic cell cultures from the hip. Our findings suggest that bone loss with aging may be accounted for, at least in part, by a decreased osteoblastic cell proliferation and an increased osteoblastic maturation. In addition, our data indicate that these changes with aging do not occur similarly at different skeletal sites.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Charcot-Marie-Tooth disease (CMT) and hereditary neuropathy with liability to pressure palsies (HNPP) are two inherited peripheral neuropathies. The most prevalent mutations are a reciprocal 1.5-Mb duplication and 1.5-Mb deletion, respectively, at the CMT1A/HNPP locus on chromosome 17p11.2. Point mutations in the coding region of the myelin genes, peripheral myelin protein 22 (PMP22), myelin protein zero (MPZ) or connexin 32 (Cx32) have been reported in CMT patients, including CMT type 1 (CMT1), CMT type 2 (CMT2) and Déjérine-Sottas neuropathy (DS) patients, and only in the coding region of PMP22 in HNPP families lacking a deletion. We have investigated point and small mutations in the MPZ, PMP22 and Cx32 genes in a series of patients of Spanish ancestry: 47 CMT patients without duplications, and 5 HNPP patients without deletions. We found 15 different mutations in 16 CMT patients (34%). Nine different mutations in ten patients were detected in the Cx32 gene, this being the most frequently involved gene in this series, whereas five mutations involved the MPZ gene and only one the PMP22 gene. Six out of nine nucleotide substitutions in the Cx32 gene involved two codons encoding arginine at positions 164 and 183, suggesting that these two codons may constitute two Cx32 regions prone to mutate in the Spanish population. Analysis of HNPP patients revealed a 5′ splicing mutation in intron 1 of the PMP22 gene in a family with autosomal dominance, which confirms allelic heterogeneity in HNPP. Ectopic mRNA analysis on leukocytes suggests that this mutation might behave as a null allele.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-482X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract CdS polycrystalline films were grown onto glass substrates by chemical bath deposition (CBD) and characterized by spectroellipsometry, X-ray diffraction and transmission electron microscopy. The X-ray diffraction patterns of the samples showed the presence of a CdS cubic phase (β-CdS) and of Cd2SiO4 as interfacial material. Using electron diffraction it was possible to index the films as cubic CdS. From effective dielectric function measurements and from reported optical data for the dielectric function of cubic CdS crystals, grown by vapour phase epitaxy, it was possible to fit the experimental data to an effective medium approximation, and to deduce the film thickness, the void fraction and the field screening.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 100 (1997), S. 15-28 
    ISSN: 1573-6857
    Keywords: antisense RNA ; methylation ; ORF codon usage ; tandem ; Zea genus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Grande1 elements constitute a family of Ty3 retrotransposons present in the Zea genus in more than 1000 copies in Zea diploperennis and maize. The sequences of three Grande1 flanking regions, two from Z. diploperennis and one from maize, reveal transposable elements as insertion targets, suggesting a preferential integration of Grande1 elements into other transposable elements. These retrotransposons are remarkable for their large size of around 14 kb, which is a consequence of a very large 3′ region of more than 7 kb. Atypical entities within this region are two arrays of unrelated tandem repeats with potential stable stem-loop structures. A large portion of the same region is occupied by ORFs, although only ORF23, whose function is unknown, is presumably transcribed in antisense orientation to the reverse transcriptase ORF. Only ORF23 has a codon usage similar to the one tabulated for highly-expressed maize genes. Correspondingly, the transcript of 900 b that hybridizes with ORF23 probes is found in all the maize tissues explored. This is despite the high level of methylation in the DNA of Grande1. Genomic RNA has not been detected in any tissue or situation studied, probably reflecting a non-functional retrotransposon. The origin of ORF23 and the remainder 3′ region might be due to a transduction event.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Two protein bands with laccase activity were found after PAGE of culture liquid or mycelium extract of Pleurotus eryngii, grown on glucose–ammonium tartrate–yeast extract medium with and without inducers. A major and a minor laccase band were observed in the basal medium. The intensity of the major band (laccase I) did not change after the addition of inducers. However, the minor band (laccase II), characterized by higher electrophoretic mobility, was strongly induced by wheat–straw alkalilignin and vanillic and veratric acids. Laccase activity in the basal medium had an optimum pH of 4.5 and was stable from pH 3 to 10 during 24 h at room temperature. This enzyme had wide substrate specificity on hydroquinones, methoxy-substituted monophenols, and aromatic amines. In general, laccase activity was found only with compounds having a redox potential lower than 0.5 mV. The highest activity was obtained with methoxy- and methyl-substituted p-hydroquinones and aromatic diamines. Some activity also occurred with the aliphatic compound 3,5-cyclohexadiene-1,2-diol.
    Type of Medium: Electronic Resource
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