ISSN:
1476-4687
Source:
Nature Archives 1869 - 2009
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Notes:
[Auszug] The bacteria were grown in Difco nutrient agar for 24 h at 29 C mechanically collected from the agar surface and washed twice by centrifugation in 1/15 M phosphate buffer, pH 6-5. They were then sonicated in a 10 kc Raytheon apparatus for 20 min, centrifuged at 12,000 r.p.m. and the supernatant was ...
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/218567a0
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