ISSN:
0449-296X
Keywords:
Physics
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Column systems for gel permeation chromatography are usually calibrated by eluting a series of sharp polymer fractions of known molecular weights and by correlating their elution volumes with molecular size or weight. A different method for calibration is proposed in which only one polymer sample, with a broad, well-characterized molecular weight distribution, is used. The cumulative distribution and the integrated, normalized GPC chromatogram are successively superimposed, and molecular weights and corresponding elution volumes are correlated. It is found that calibration curves obtained in this manner show a definite curvature. A possible explanation and correction for this behavior is given, based on the concentration dependence of elution volumes.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/pol.1967.150050619
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