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  • Cell & Developmental Biology
  • EARTH RESOURCES AND REMOTE SENSING
  • 1985-1989
  • 1965-1969  (14)
  • 1960-1964
  • 1950-1954
  • 1966  (14)
Collection
Keywords
Publisher
Years
  • 1985-1989
  • 1965-1969  (14)
  • 1960-1964
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    Histochemical observations on mucin secretion by subsurface epithelial structures in the canine ovary (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 120 (1966), S. 347-358 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Histochemical methods for mucins were applied to the ovaries of 23 dogs.Solid and hollow groups and cords of epithelial cells (subsurface epithelial structures, SES) in the outer part of the cortex regularly showed evidence of mucin secretion. Intracytoplasmic, sialic acid-containing, acid mucin secretion droplets were seen in solid and hollow SES, and secretion was present in both closed lumina and those opening onto the surface.Intracytoplasmic droplets in the cells of SES were distinctive, and similar droplets were not found in the cells of any other ovarian epithelial component. The secretion of SES was not shown to possess distinctive histochemical features.Mucin production was also observed in follicles, corpora lutea and rete tubules.The significance of ingrowth from the ovarian surface epithelium in adult life, and of secretory activity by the cells of SES, are discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051200403
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  • 2
    Electronic Resource
    Electronic Resource
    Development of the gonads in Channa punctatus bloch (osteichthyes: Channidae) (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 119 (1966), S. 467-475 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The history of the germ cells is traced from the time of hatching. The germ cells are larger in size and have faintly staining cytoplasm, clear cell outline and a distinct nucleus. They migrate by ameboid movement to reach the genital ridge and aggregate to lie against the gonadal epithelium prior to the formation of gonads. The germ cells are distributed along the gonad primordia.The period of sex differentiation occurs between the 5.4 mm to 12 mm stage. The testis formation is recognized by the presence of germ cell nests and the sperm duct cord. The formation of the ovary is noted by the enlargement of the germ cells of uniform size and the development of the ovarian cavity.The ovaries are described in four stages ranging from 21 mm to 135 mm fish. At 21 mm stage the ovarian cavity is continuous but is obliterated at 35 mm stage due to the projection of the ovigerous lamellae. The common opening for both the ovaries develops at 35 mm stage. The testes are described in four stages ranging from 23 mm to 135 mm fish. They differentiate more slowly and the first maturation division is seen at 90 mm stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051190406
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  • 3
    Electronic Resource
    Electronic Resource
    Fine structure and origin of the tunic of Perophora viridisSupported by grants HD-00143, 2TI-GM406, and HD-30097 from the United States Public Health Service. (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 120 (1966), S. 267-280 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fine structure of the tunic of a typical ascidian was investigated because of the cellulose-like polysaccharide known to occur in its substance. The glycoprotein mantle does contain filaments very much like plant cellulose in morphology. Tunicin filaments are 35-50 Å in diameter, often beaded, and of indeterminate length. Histochemical evidence that they are composed of cellulose is given here and past chemical and physical studies on the unusual ascidian polysaccharide are reviewed. Moreover, we present here for the first time direct autoradiographic evidence that epidermal cells are involved in the synthesis and secretion of tunicin. Tritiated glucose is immediately incorporated into the Golgi zone of epidermal cells and labeled product appears in the tunic at later intervals. The fine structure of the epidermal cell is described in detail. Unlike the rather moribund appearing vanadocyte that wanders through the tunic, the epidermal cell has well-developed cytoplasmic organelles and a large vesicular nucleus. The granular endoplasmic reticulum is abundant and the Golgi complex is highly developed. It seems likely that the lamellae and vesicles of the Golgi complex are involved in the production of the tunic sugar and that tunic proteins of as yet unknown nature are produced by the ergastoplasm. Further investigation of the ascidian mantle should be of interest because of the possibility that cellulose is a more general component of glycoprotein surface coats in animals than has heretofore been recognized.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051200304
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  • 4
    Electronic Resource
    Electronic Resource
    Reversal of primary embryonic induction by cold, and the “fixation” of induction in cells activated by lithium chloride (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 118 (1966), S. 1-9 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of low temperature on embryonic induction were investigated. Presumptive epidermis cells of Rana pipiens were either pretreated with cold (4°C) and then treated with lithium chloride at the same temperature, or activated by lithium chloride at 22°C, then rapidly chilled to 4° and kept at this temperature for a period of time. Cultures were made from the treated cells.It was found that induction at low temperature could occur, although it did not proceed beyond determining neuralization. When, on the other hand, the cells were first induced with LiCl to the point at which they should have been determined to differentiate into melanophores, but were then rapidly chilled and kept in the cold for some time, the effects of induction were suppressed and the cells differentiated into ciliated epithelium. A 15-60 minute lag in time between induction and chilling (during which the cells were kept in the culture medium at room temperature) allowed neural induction to be gradually “fixed,” and a 120-180 minute lag was sufficient for melanphore induction to become insensitive to the subsequent treatment with cold.It is speculated from these results that induction occurs very slowly at low temperature and that the inductive stimulus activates a sequence of reactions, one or several of which are strongly repressed by the cold. In this case, when no products result from these reactions, the sequence is assumed to stop and possible feed-back mechanisms switch the whole system to its initial condition.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051180102
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  • 5
    Electronic Resource
    Electronic Resource
    A new intranuclear inclusion body in the frog renal adenocarcinomaThis investigation was supported in part by a research grant (CA 07048) from the National Institutes of Health, Public Health Service, Bethesda, Maryland. (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 118 (1966), S. 581-587 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology and possible functional significance of a new intranuclear inclusion body associated with the frog renal adenocarcinoma is described. The inclusion consists of particles, 40 to 50 mμ in diameter, arranged in an orderly pattern suggestive of a crystalline array. Individual particles eventually appear to be involved in the production of “doughnut” type virions characteristically associated with this malignancy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051180410
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  • 6
    Electronic Resource
    Electronic Resource
    The terpene-producing glands of a phasmid insect cell morphology and histochemistry (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 119 (1966), S. 143-159 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The defensive glands of Anisomorpha buprestoides produce the terpene toxicant anisomorphal. Each gland consists of a cuticular secretion reservoir surrounded by the secretory epithelium and the musculature which serves to compress the gland and expel the secretion. Two types of cells make up the secretory epithelium: a squamous layer next to the cuticular reservoir and a layer of larger secretory cells responsible for production of the toxicant. The microvilli-laden plasma membrane of each secretory cell is invaginated to form a central cavity. It appears that secretory products pass into the central cavity and then flow out to the gland reservoir via an efferent cuticular ductule contained within the squamous epithelial cell.Histochemical techniques demonstrate lipid reserves, carboxylic esterases, a variety of phosphatases, and an alcohol dehydrogenase, within the secretory cells. It is suggested that the lipid reserves are precursors of the terpenoid toxicant, that a mevalonic kinase has been histochemically demonstrated by the phosphatase test, and that an unusual alcohol dehydrogenase is active in the final steps of toxicant synthesis. The histochemical evidence is consistent with the hypothesis that anisomorphal is produced via the mevalonic acid pathway.
    Additional Material: 28 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051190204
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  • 7
    Electronic Resource
    Electronic Resource
    Acid-base, fluid and electrolyte metabolism in the elasmobranchThis work was supported in part by a grant (HE-05059) from the National Institutes of Health, and in part by a grant from the National Science Foundation.. III. oxygen, CO2, bicarbonate and lactate exchange across the gill (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 93-100 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Methods have been developed which permit measurements of cardiac output and sampling of mixed venous and arterial blood in the elasmobrach, Squalus acanthias. These methods have been utilized to characterize some of the parameters involved in gill gas exchange. A total of 17 animals have been studied with the following results. Cardiac output: 1.49±0.57 1/kg/hr.; V̇o2: 38.3±22.1 ml/kg/hr.; V̇co2: 32.1±19.0 ml/kg/hr.; RER: 0.92±0.17; PaO2: 104±27 mmHg; Pv̄o2: 18±8.8 mmHg; PaCO2: 2.9±0.75 mmHg; Pv̄co2: 4.6±1.1 mmHg; CaO2: 3.8±0.75 vol. %; Cv̄o2: 1.26±0.59 vol. %; pHa: 7.52±0.10 units; Pv̄: 7.42±0.11 units; [HCO3-]a: 3.92± 1.14 meq/1; [HCO3-]v̄: 4.80±1.15 meq/1; [dissolved CO2]a: 0.17± 0.04 mM/1; [dissolved CO2]v̄: 0.24±0.04 mM/1; [lactate-]a: 13.47±7.17 meq/1; [Lactate-]v̄: 13.01±8.18 meq/1. Some of the mechanisms involved in gill gas exchange have been characterized.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040670111
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  • 8
    Electronic Resource
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    The release of amino acids from nerve during stimulationSupported by grant NB03971-03 from the USPHS. (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 141-147 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Frog sciatic nerves were incubated for 24 hours in either glycine, aspartic acid, glutamic acid, lysine, leucine, γ-aminobutyric acid, glutamine, or pentanedioic acid (all labeled with C14), and the rates of release of these compounds were monitored under resting conditions and during stimulation. Upon stimulation, the rate or release of glutamic acid increased an average of 200% above the resting rate. This extra release is highly specific with regard to molecular size and structure, since of the compounds tested only glutamic acid gave significant increases in rates of release during stimulation. Ouabain (0.1 mM) had no effect on the rate of release; however, sodium azide (0.2 mM or 1.0 mM) completely eliminated the extra release during excitation, indicating that the increased permeability to glutamic acid is energy-dependent. Competition experiments show that the extra release of glutamic acid can be eliminated with 10 mM concentrations of non-isotopic choline. The hypothesis is advanced that glutamic acid is actively extruded by a highly specific carrier mechanism.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040670116
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of hematoporphyrin and light on human plateletsThis investigation was supported by a research grant he 09102-02 from the national heart institute, bethesda, maryland.Presented in part at the meeting of the federation of american societies for experimental biology in april, 1964 and abstracted in fed. proc., 24: 519, 1965.. I. Morphologic, functional, and biochemical changes (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 271-279 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphologic, functional, and biochemical changes produced by hematoporphyrin and light in human platelets have been characterized. by phase microscopy the cells appeared swollen and resembled signet rings; by electron microscopy they showed considerable loss of cytoplasm and their contour was smoother than normal. irradiated platelets were not aggregable by thrombin and calcium chloride, although they contained clottable protein, and were incapable of supporting clot retraction. a linear relationship was demonstrated between the per cent depletion of serotonin from irradiated platelets and the log dose of hematoporphyrin. the depletion of serotonin from these platelets was related lineraly to the log of time of exposure to light during the initial six minutes of exposure; but thereafter continued at a constant rate. the temperature of incubation influenced directly the rate of depletion of serotonin from irradiated platelets but did not influence the movement of serotonin into these platelets. atp was diminished considerably in irradiated platelets. these changes are attributable to damage to the membrane of the platelet by hematoporphyrin and light.These studies provide additional information about the blood platelet in terms of its response to photodynamic action.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040670207
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  • 10
    Electronic Resource
    Electronic Resource
    The effect of hematoporphyrin and light on human plateletsThis investigation was supported by a research grant he 09102-02 from the national heart institute, bethesda, maryland.Presented in part at the meeting of the federation of american societies for experimental biology in april, 1965 and abstracted in fed. proc., 24: 519, 1965.. II. Uptake of hematoporphyrin (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 281-284 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A linear relationship was demonstrated between the reciprocals of the concentration of free hematoporphyrin and the moles of hematoporphyrin taken up by the platelet in the dark. radiated platelets took up more hematoporphyrin than did controls; this increase in uptake was accounted for by the movement of the dye across the damaged membrane of the cell. platelets irradiated at 4°c remained impermeable to hematoporphyrin until warmed to 37°c. during the initial three to four minutes of exposure to light at 37°c, there was no additional uptake of hematoporphyrin by platelets in comparison to controls. between six to ten minutes irradiation, the uptake of hematoporphyrin increased linearly with the log time of irradiation. thereafter, no further uptake occurred. a further increase in uptake of dye was demonstrated by both control and irradiated platelets at a reduced ph. this study enables a correlation to be made between the effects of hematoporphyrin on the platelet and the uptake of this agent by the platelet.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040670208
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