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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 4 (1957), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. The endogenous development of the life cycle of Eimeria alabamensis Christensen, 1941, occurs in the nucleus of the intestinal cells of cattle. Calves were killed at various intervals after inoculation with infective oöcysts to study the endogenous cycle. Excysted sporozoites were found in the contents or scrapings from the walls of rumen, omasum, small intestine, cecum, and colon. They were found in the cytoplasm of intestinal epithelium at 2 days. Schizonts were found in the nuclei beginning at 2 days, but the number was low by the 8th day. Merozoite numbers usually ranged between 16 and 32. Some host nuclei contained as many as 48 or more, but these appeared to be the result of more than one schizont merging in the same host nucleus. Merozoites were slender, spindle-shaped bodies while still in the schizont walls, but were short with bluntly rounded tips when found in intracellular spaces and crypts. Gametocytes were found as early as the 4th day. Most of the stages of gametogenesis were limited to the lowest third of the small intestine, but in heavy infections some were also found in the cecum and upper colon. Microgametocytes were multinucleate and were more densely stained than the uninucleate macrogametocytes. The ratio of macrogametocytes to microgametocytes in 100 gametes was 78: 22. Oöcysis with “shells” were found in sections of the lower 20 feet of the ileum on the 6th day, which coincided with the shortest prepatent period reported previously. As many as three schizonts or microgametocytes or four or five macrogametocytes or oöcysts could be found in the same host nucleus. The variations in shape of the oöcysts appeared to be dependent on the number of oöcysts crowded into each nucleus.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 6 (1959), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Differences in temperature-tolerance, generation time, nutritional requirements, osmoresistance, pigment production, and sensitivity to high hydrogen or hydroxyl ion concentration have been observed among varieties, and among mating types within varieties of Tetrahymena pyriformis. The usefulness of these differences to taxonomists and geneticists is discussed.The nutrition of a temperature-tolerant strain, mating type II, variety 1, was given special attention and a chemicallydefined medium devised to support rapid growth at 35d̀ C. At 40d̀, growth in this medium failed, but was good in a crude medium. Temperature factors are suggested. Permeability of this ciliate, as inferred by utilization of nucleotides, was high.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 6 (1959), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. In cattle fed a high-starch diet, species of Entodinium and Diplodinium ingested associated ruminal bacteria. Stained preparations of diluted rumen contents showed Entodinium caudatum, E. minimum, E. dubardi, (syn. E. simplex), E. longinucleatum, E. bursa, E. nanellum, E. exiguum, and E. vorax contained gram-positive diplococci. Starch grains with adherent gram-positive diplococci were observed within Entodinium spp. Diplodinium ecaudatum forma ecaudatum, D. ecaudatum forma caudatum, D. neglectum and an unidentified species of Diplodinium also ingested ruminal diplococci. Bacteria were isolated from mixed species of Entodinium by washing and culturing the protozoa in a starch feed-extract agar medium. The strains isolated from the ciliates were gram-positive diplococci, 0.8 times 1–1.5 μm, which attached themselves to starch granules and were able to digest the starch. Conclusive evidence of bacterial ingestion by the oligotrichs was obtained by providing the bacterial cultures to Entodinium species (E. dubardi and E. minimum) which had been starved 24 hr. Gram-stained preparations showed the ciliates readily ingested the bacteria. The amylolytic cocci utilized by Entodinium spp. were identified as Streptococcus bovis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 83 (1959), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 80 (1959), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 65 (1957), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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