ISSN:
1432-0983
Keywords:
mtDNA of Saccharomyces cerevisiae
;
rho−restriction mapping
;
Intervening sequence mutations
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A physical map of the COB region in mtDNA of yeast has been established. This region harbours a split gene coding for apocytochrome b. It includes restriction sites of seven endonucleases (EcoRI, HindII, HindIII, HaeIII, HpaII, AluI and BamHI). Various mtDNA sequences of this region, retained in a series of genetically characterized rho− clones have been allocated to this map. The combination of this physical map with a genetic map of the rho− clones revealed that 1) cob− mutational sites spread over 8,400 bp, 2) mutations in sequences coding for apocytochrome b map in five distinct segments which are separated by intervening sequences with minimum lengths from 350 to 1,900 bp.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00419582
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