ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Unagitated cultures of Acanthamoeba in the logarithmic growth phase have been observed to consist of over 99% mononucleates and less than 1% multinucleates. A small increase in the relative numbers of multinucleates occurs as the culture age and conditions for growth become limiting. A large increase in the proportion of multinucleates occurs during logarithmic growth, if the cultures are agitated by shaking or by the bubbling from a stream of air. After an initial increase and until the end of logarithmic growth, the numbers of multinucleates in the agitated cultures are maintained at a constant fraction of the total population. Proportions of multinucleates as high as 25% are observed during this period. A second increase in the relative numbers occurs as conditions for growth become limiting. Under these conditions, the multinucleates may exceed 50% of the total population. The factors inducing multinuclearity as well as the kinetics of the mononucleate and multinucleate accumulation curves are discussed.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040700108
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