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  • Azotobacter vinelandii  (2)
  • DNA  (2)
  • Springer  (4)
  • American Institute of Physics
  • Wiley
  • 1985-1989
  • 1970-1974  (4)
  • 1965-1969
  • 1935-1939
  • 1974  (4)
  • 1937
Collection
Keywords
Publisher
  • Springer  (4)
  • American Institute of Physics
  • Wiley
Years
  • 1985-1989
  • 1970-1974  (4)
  • 1965-1969
  • 1935-1939
Year
  • 1
    Electronic Resource
    Electronic Resource
    Genome size and the proportion of repeated nucleotide sequence DNA in plants (1974)
    Springer
    Biochemical genetics 12 (1974), S. 257-269 
    Details
    ISSN: 1573-4927
    Keywords: DNA ; repeated sequences ; plant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The reannealing kinetics of denatured DNA fragments from 23 species of higher plants have been studied, using hydroxylapatite chromatography to distinguish reannealed from single-stranded DNA. The 2C nuclear DNA contents of the species varied between 1.7 and 98 pg. The proportions of DNA in species with a nuclear DNA mass above 5 pg that reannealed with the kinetics of sequences present in more than 100 copies were high (69–92% with a mean of 80±2.0%). For species with less than 4 pg of DNA, the mean proportion of repeated-sequence DNA was 62±2.9%. It is concluded that most of the variation in nuclear DNA mass in higher plant chromosomes can be accounted for by variation in repeated-sequence DNA. The consequences of altering the adapted DNA content of a species by the addition of families of repeated sequences are discussed in relation to the proportion of repeated-sequence DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00485947
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of nitrogen source on growth and nitrogenase activity inAzotobacter vinelandii (1974)
    Springer
    Archives of microbiology 96 (1974), S. 135-144 
    Details
    ISSN: 1432-072X
    Keywords: Azotobacter vinelandii ; Nitrogenase Activity ; Acetylene Reduction Test ; Growth ofAzotobacter ; Regulation of Nitrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and nitrogenase activity were studied in cultures ofAzotobacter vinelandii growing with dinitrogen, ammonium sulfate, aspartic acid or yeast extract. Nitrogenase activity was measured by means of the C2H2 reduction test. In the presence of ammonium sulfate nitrogenase is completely repressed. After exhaustion of ammonia its activity is restored following a diauxic lag period of 30 min. With aspartic acid nitrogenase activity is partially repressed, and growth yield is higher than in the culture growing with N2 only. This is due to simultaneous use of dinitrogen and aspartate. Fluctuations of nitrogenase activity occurring during exponential growth and the mechanism of their regulation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00590170
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative relations for the repression of nitrogenase synthesis in Azotobacter vinelandii by ammonia (1974)
    Springer
    Archives of microbiology 101 (1974), S. 153-159 
    Details
    ISSN: 1432-072X
    Keywords: Azotobacter vinelandii ; Nitrogenase ; Repression ; Ammonia Determination ; Oxygen Effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described which allows the quantitative determination of small ammonia concentrations in the culture of nitrogen-fixing microorganisms. With this method the ammonia concentration range was estimated in which repression of nitrogenase synthesis in Azotobacter vinelandii occurs. Both in batch and continuous cultures there was no repression below 10 μM, whereas nitrogenase synthesis stopped completely if the ammonia concentration in the medium exceeded 25 μM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455935
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  • 4
    Electronic Resource
    Electronic Resource
    Kinetics of reassociation of DNA from the thymus and ascites hepatoma of rats (1974)
    Springer
    Bulletin of experimental biology and medicine 78 (1974), S. 1311-1312 
    Details
    ISSN: 1573-8221
    Keywords: normal cells ; tumor cells ; DNA ; molecular hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The kinetics of renaturation of DNA from the rat thymus and ascites hepatoma was studied. Three zones were distinguished on the curve, corresponding to fast, intermediate, and slow rates of DNA reassociation. The course of the reassociation curves of DNA from tumor and normal tissues did not diverge in any of these zones. Consequently, no difference could be detected between the DNA's of normal and neoplastic cells on the basis of their reassociation kinetics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00804369
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