ISSN:
1432-2242
Keywords:
Molecular markers Beet cyst nematode resistance
;
Hs1 Heterodera schachtii Schm
;
Satellite DNA Monosomic fragment additions Beta
;
Pulsed-field gel electrophoresis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 pat-1 locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 pro-1 locus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00225381
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