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  • Cell & Developmental Biology  (144)
  • Chemical Engineering  (91)
  • Wiley-Blackwell  (235)
  • 1985-1989  (233)
  • 1925-1929  (2)
  • 1989  (233)
  • 1928  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Optical fiber chemical sensors utilizing dye-doped silicone polymer claddings (1989)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 29 (1989), S. 1215-1218 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Transparent dimethyl siloxane network polymers with refractive indices near 1.40 may be applied to fused silica fibers (n = 1.458) as they are drawn to produce plastic-clad silica (PCS) optical fibers. The evanescent tail of the light energy propagating in the core of such fibers extends into the silicone cladding, where it interacts with chemical species present in the polymer. If the silicone is doped with a dye, the absorption spectrum or fluorescence spectrum of the dye is reflected in the transmission spectrum of the fiber. Further, if the dye changes its absorption spectrum or fluorescence spectrum as a result of diffusion of a chemical species into the silicone, the change is detectable in the fiber output. The polymer material properties which determine the performance of these sensors are described, along with examples of sensors for ammonia and oxygen which utilize either color changing or fluorescent dyes.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760291715
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of sulfated glycoconjugates on capacitation and the acrosome reaction of bovine and hamster spermatozoa (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 24 (1989), S. 403-413 
    Details
    ISSN: 0148-7280
    Keywords: heparin ; fertilization ; dextran sulfate ; fucose sulfate glycoconjugate ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of sulfated glycoconjugates on the preparation of mammalian sperm for fertilization were investigated. The three sulfated glycoconjugates tested were heparin, dextran sulfate, and the fucose sulfate glycoconjugate (FSG) from the sea urchin egg jelly coat. In vivo, FSG induces the acrosome reaction in sea urchin sperm. Bovine sperm were found to be capacitated by heparin and FSG as judged both by ability of lysophosphatidylcholine (LC) to induce an acrosome reaction and by ability to fertilize bovine oocytes in vitro. The mechanism by which heparin or FSG capacitated bovine sperm appeared similar, since glucose inhibited capacitation by both glycoconjugates. In contrast to effects on bovine sperm, heparin and FSG induced the acrosome reaction in capacitated hamster sperm. When hamster sperm were incubated under noncapacitating conditions, heparin had no effect on capacitation or the acrosome reaction. Three molecular weights (MW) of dextran sulfate (5,000, 8,000, 500,000) were found to capacitate bovine sperm as judged by the ability of LC to induce an acrosome reaction. Whereas bovine sperm incubated with 5,000 or 8,000 M W dextran sulfate fertilized more bovine oocytes than control sperm (P 〈0.05), sperm treated with 500,000 M W dextran sulfate failed to penetrate oocytes. The high-MW dextran sulfate appeared to interact with the zona pellucida and/or sperm to prevent sperm binding. Results suggest that sulfated glycoconjugates may prepare sperm for fertilization across a wide range of species.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120240407
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  • 3
    Electronic Resource
    Electronic Resource
    Ontogeny of gut morphology in the white shrimp Penaeus setiferus (Decapoda, Penaeidae) (1989)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 201 (1989), S. 253-272 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ontogeny of the gut in Penaeus setiferus was investigated by reconstruction of serial sections examined by light microscopy. Development of the gut into the adult form is protracted over several weeks beyond metamorphosis in steps that may be directly related to the unique postlarval life history of Penaeus. The gastric mill is lacking in larval stages of P. setiferus. In protozoeal stages Z1-Z3, the pyloric ampullae are blind sacs that do not communicate with the midgut. The gland filter first appears in mysis stage M2. The gastric mill in early postlarval (PL) stages consists of poorly chitinized lobes with flexible setae. By PL21 the ossicles of the gastric mill are rigid and setae are replaced by spine-like denticles, but even by PL35 the gastric mill is neither as massive nor heavily chitinized as in adults. During the mysis stages and early PL stages, the hepatopancreas communicates freely with both the foregut and the midgut trunk. By PL35 the hepatopancreatic ducts are essentially isolated from the remainder of the midgut by foregut ossicles.The midgut in Z1 consists of two pairs of simple caeca and the midgut trunk. During larval growth, each of the lateral midgut caeca develops into a number of lobes. After metamorphosis these lobes begin to ramify into small-diameter tubules, and by PL35 have completely ramified into the hepatopancreas of adults. From M1 to PL4, the anterior midgut caeca decrease in absolute size and become a single anterior diverticulum. The posterior midgut diverticulum first appears in PL21 as a simple sac and thereafter increases in size and complexity.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052010305
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  • 4
    Electronic Resource
    Electronic Resource
    Interacting genes identify interacting proteins involved in microtubule function in Drosophila (1989)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 14 (1989), S. 128-135 
    Details
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970140122
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  • 5
    Electronic Resource
    Electronic Resource
    Fluid mixing with a pipeline tee: Theory and experiment (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 406-414 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The pipeline mixing of two fluid streams by turbulent jet injection normal to the pipeline has been studied theoretically and experimentally. A simple scaling law for the second moment of the tracer concentration within the pipeline is proposed for the first fifteen pipe diameters down-stream from the injection point. The similarity solution is derived by assuming that the tracer diffuses in a weak compound jet moving parallel to the pipeline axis. The theoretical results are correlated with all of the available experimental measurements. The results indicate that the second moment of the tracer concentration decreases with increasing jet momentum and distance from the injection point such that M = 0.25. (lm/D)-2 (x/D)-4/3.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690350308
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  • 6
    Electronic Resource
    Electronic Resource
    Laser photometric probe for concentration measurements in liquid dispersions (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 507-510 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690350319
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  • 7
    Electronic Resource
    Electronic Resource
    Laser capillary spectrophotometry for drop-size concentration measurements (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 1073-1084 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A laser capillary spectrophotometric technique (LCS), based on the method of Verhoff et al. (1969, 1977), is developed for the rapid and accurate determination of the bivariate drop size-concentration distribution for reactive liquid-liquid dispersions. The method employs a precision bore capillary, through which a sample is drawn from the mixing vessel by a vacuum pump. An appropriate light source is used to distinguish between the slugs of dispersed phase and continuous phase as the sample passes by two detection points in the capillary. The optical device is designed to measure drop size by the difference of light refraction between the two phases and drop concentration by the light absorbance of the organic phase in the drop. The new technique can automatically measure drop-size and drop-concentration distributions of thousands of drops within a few minutes. This technique can provide important information on the state of a reactive dispersion in situ and can provide information on the influence of dispersed-phase mixing effects on mass transfer and/or reactions in liquid-liquid dispersions. Further, the LCS technique provides the capability for on-line monitoring and control of units processing liquid dispersions.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690350703
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  • 8
    Electronic Resource
    Electronic Resource
    Modulation of TGF-β type 1 receptor: Flow cytometric detection with biotinylated TGF-β (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 141 (1989), S. 170-180 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Transforming growth factor β- type 1 (TGF-β) was reacted with NHS-biotin to yield a derivative of TGF-β1 which was biotinylated on lysine residues. The biotinylated form of TGF-β1 was separated from the unreacted material by reverse phase chromatography. In three separate bioassays, the derivatized peptide was as active as the starting material. The use of FITC-avidin in conjunction with flow cytometry demonstrated that the binding of biotinylated TGF-β to its receptor is saturable, competable, and specific. A 100-fold molar excess of unde-rivatized TGF-β1 gave 85% inhibition of binding of the biotinylated peptide to the mink lung cell line CCL-64, while TGF-β2 showed no inhibition of binding, nor did insulin, calcitonin, or TGF-α. Both CCL-64 cells and human umbilical vein endothelial cells showed a density-dependent down-regulation of receptor expression in culture. Several factors were examined that might mediate this effect. The down-regulation was shown not to be due to the secretion of an active form of TGF-β1. The extracellular matrix from high-density cells did not decrease expression of the receptor. Fibronectin, collagen, and gelatin were also unable to signal changes in receptor expression, even though in other systems such matrix components can regulate the responsiveness of cells to TGF-β1. Lastly, staining simultaneously for DNA content and TGF-β1receptor expression showed that there was no correlation between cell cycle and receptor levels.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041410125
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  • 9
    Electronic Resource
    Electronic Resource
    Coordination of keratinocyte programming in human SCC-13 squamous carcinoma and normal epidermal cells (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 138 (1989), S. 208-214 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Exploiting the sensitivity of neoplastic keratinocytes to physiological effectors, this work analyzes the degree of coordination among differentiation markers in the established human epidermal squamous carcinoma cell line SCC-13 in comparison to normal human epidermal cells. This analysis showed that overall keratin content was modulated substantially and in parallel with particulate transglutaminase activity in response to variation of calcium, retinoic acid, and hydrocortisone concentrations in the medium. The changes in keratin expression were evident primarily in the striking stimulation by hydrocortisone or calcium and the virtual suppression by retinoic acid of species in the 56-58 kd region, which have not previously been reported subject to such physiological modulation. In contrast, involucrin levels were coordinated only to a limited degree with particulate transglutaminase activity and keratin content. The very low involucrin levels observed in low calcium medium were increased 5- to 10-fold in high calcium medium. However, they were also increased 5- to 30-fold in low calcium medium by retinoic acid, a clear example of uncoupling. Activities of the tissue transglutaminase were altered considerably by the various culture conditions but were not obviously coordinated to keratinocyte markers. In normal epidermal cells, the suppressive effect of retinoic acid was much more evident with particulate transglutaminase than involucrin levels. While calcium had a large stimulatory effect on both markers, hydrocortisone had little or no influence. These results emphasize the potential importance of quantitative analysis of differentiation markers for resolving the contribution of physiological elements in coordination of cellular programming.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041380128
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  • 10
    Electronic Resource
    Electronic Resource
    Regulation of cerebroside sulfotransferase activity in cultured oligodendrocytes: Effect of growth factors and insulin (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 141 (1989), S. 667-674 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cerebroside sulfotransferase (EC 2.8.2.11, CST) specific activity has been determined in oligodendrocyte (OL)-enriched glial cell cultures from newborn rat brain grown in serum supplemented medium. This activity is detectable at 5 days in vitro (DIV) and reaches its maximum value at 12 DIV. This period corresponds to that of oligodendrocyte precursor proliferation in these cultures. The activity decreases thereafter and remains nearly constant after 24 DIV. The developmental curve of CST activity is parallel in pure oligodendrocyte subcultures but twice higher than in primary cultures. These data confirm that CST is highly enriched in OL. Basic fibroblast growth factor (bFGF) (15 ng/ml) and platelet derived growth factor (PDGF) (0.75 U/ml) both enhance CST activity by 90% and 72%, respectively. This increase is in the same range than that of DNA content in treated cultures, whereas protein increase is smaller (50% and 22%, respectively). In contrast, transforming growth factor β1 (TGFβ1, 0.5 and 5 ng/ml) does not significantly enhance CST activity nor DNA content of OL cultures. Insulin at high concentrations (5 μg/ml) also enhances CST activity but has no effect at physiological concentrations (20 ng/ml). These results show that CST activity can be controlled by growth factors. They suggest that CST activity is more closely related to OL and OL precursor proliferation than to myelination itself since its maximal activity preceeds myelination in vitro.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041410327
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