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Digitale Medien

Nongenomic regulation of extracellular matrix events by vitamin D metabolites (1994)

Boyan, Barbara D. ; Dean, D. D. ; Sylvia, V. L. ; [weitere]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 56 (1994), S. 331-339

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ISSN: 0730-2312

Schlagwort(e): 1,25-(OH)2D3 ; 24,25-(OH)2D3 ; matrix vesicles ; nongenomic regulation ; extracellular matrix ; alkaline phosphatase ; phospholipase A2 ; Protein kinase C ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Vitamin D metabolites appear to regulate chondrocytes and osteoblasts via a combination of genomic and nongenomic mechanisms. Specificity of the nongenomic response to either 1,25-(OH)2D3 or 24, 25-(OH)2D3 may be conferred by the chemical composition of the target membrane and its fluid mosaic structure, by the presence of specific membrane receptors, or by the interaction with classic Vitamin D receptors. Nongenomic effects have been shown to include changes in membrane fluidity, fatty acid acylation and reacylation, arachidonic acid metabolism and prostaglandin production, calcium ion flux, and protein kinaase C activity. Chondrocytes metabolize 25-(OH)D3 to 1,25-(OH)2D3 and 24,25-(OH)2D3; production of these metabolites is regulated by both growth factors and hormones and is dependent on the state of cell maturation. 1,25-(OH)2D3 and 24,25-(OH)2D3 may interact directly with extracellular matix vesicles to regulate their function in the matrix, including protease activity, resulting in matrix modefication and calcification. Isolated matrix vesicles, produced by growth zone chondrocytes, can activate latent transforming growth factor-β when incubated with exogenous 1,25-(OH)2D3. These observations suggest that nongenomic regulation of martix vesicle structure and function may be a mechanism by which mesenchymal cells, like osteoblasts and chndrocytes, may modulate events in the extracellular matrix at sites distant from the cell surace.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcb.240560309

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2

Digitale Medien

Torsional properties of healed canine diaphyseal defects grafted with a fibrillar collagen and hydroxyapatite/tricalcium phosphate composite (1994)

Zardiackas, Lyle D. ; Teasdall, Robert D. ; Black, R. John ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Applied Biomaterials 5 (1994), S. 277-283

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ISSN: 1045-4861

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin , Technik allgemein

Notizen: The need for alternatives to autogenous bone grafts is widely recognized. This study compared the torsional strength of canine femora 1 year after grafting with one of three forms of a collagen/hydroxyapatite/tricalcium phosphate bone grafting material (COLLAGRAFT15), autogenous bone, or no graft. The groups were compared to each other and to the unoperated contralateral femora. Results of torsional testing were evaluated for torsional strength, torsional displacement, total energy to fracture and White fracture mode. Data analysis showed lower torsional strength of the operated vs. unoperated femora with the exception of morsellized COLLAGRAFT15 material, which had higher strength. However, the only difference in the operated groups was that the morsellized COLLAGRAFT15 had greater strength than several groups including the autogenous bone group. There was no difference found in angular displacement between any of the groups. However, there was a difference in the energy to fracture in both strip forms of the COLLAGRAFT15. The final conclusion is that in this model, grafting with COLLAGRAFT15 provided torsional properties at one year postoperatively at least equivalent to autogenous bone. © 1994 John Wiley & Sons, Inc.

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jab.770050402

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3

Digitale Medien

Metal ion concentrations in retrieved polyethylene total hip inserts and implications for artifactually high readings in tissue (1994)

Meldrum, Russell D. ; Bloebaum, Roy D. ; Dorr, Lawrence D.

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 28 (1994), S. 405-405

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ISSN: 0021-9304

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin , Technik allgemein

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jbm.820280316

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4

Digitale Medien

Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)

Garner, D. L. ; Johnson, L. A. ; Lake, S. ; [weitere]

New York, NY : Wiley-Blackwell

Gamete Research 10 (1984), S. 339-351

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ISSN: 0148-7280

Schlagwort(e): spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrd.1120100402

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5

Digitale Medien

Identification and quantification of Cu contamination on GaAs surfacesPaper presented at QSA 7, Guildford, September 1992. (1994)

Sykes, D. E. ; Chew, A. ; Hall, D. D. ; [weitere]

Chichester [u.a.] : Wiley-Blackwell

Surface and Interface Analysis 21 (1994), S. 51-55

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ISSN: 0142-2421

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Physik

Notizen: Secondary ion mass spectrometry has been used to identify the presence of Cu contamination on undoped semiinsulating GaAs wafer surfaces following polishing under certain conditions. The presence of a contaminating species on the wafer surface is indicated by the influence of pre-etch treatments on electrical measurements performed on heat-treated wafers, with and without Si implants. The identification of Cu as a contaminant is confirmed by photoluminescence measurements. With the appropriate wafer preparation process, the level of Cu contamination is below the SIMS detection limit of 〈1 × 1016 atoms cm-3.In the SIMS analysis of the surface-contaminated wafers, the Cu depth profile shape was dependent on the primary beam bombardment energy, suggesting a chemical segregation of Cu out of the altered layer, a redistribution that is known to occur in Si.To quantify the surface concentration of Cu, GaAs wafers were deliberately contaminated with Cu, heat treated and analysed with glow discharge mass spectrometry and SIMS to provide a cross-calibration.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/sia.740210108

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6

Digitale Medien

Zickzackförmige Kanäle in der Struktur von Sebacinsäuredinitril/HarnstoffDiese Arbeit wurde vom Petroleum Research Fund, verwaltet von der American Chemical Society (Nr. 24396-AC4), vom Natural Sciences and Engineering and Research Council of Canada und von der Alfred P. Sloan Foundation gefördert. Dr. John C. Huffman und Dr. Natsuko Cyr danken wir für ihre Hilfe.,In memoriam Margaret C. Etter (1994)

Hollingsworth, Mark D. ; Santarsiero, Bernard D. ; Harris, Kenneth D. M.

Weinheim : Wiley-Blackwell

Zeitschrift für die chemische Industrie 106 (1994), S. 698-701

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ISSN: 0044-8249

Schlagwort(e): Chemistry ; General Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/ange.19941060621

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7

Digitale Medien

Increase in copy number of an integrated vector during continuous culture of Hansenula polymorpha expressing functional human haemoglobin (1994)

Gilbert, S. C. ; van Urk, H. ; Greenfield, A. J. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Yeast 10 (1994), S. 1569-1580

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ISSN: 0749-503X

Schlagwort(e): Hansenula ; haemoglobin ; integration ; continuous culture ; Life and Medical Sciences ; Genetics

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: Recombinant human haemoglobin A (rHbA) was produced by a leucine-requiring strain of Hansenula polymorpha which had been transformed with an integration vector containing the Saccharomyces cerevisiae LEU2 gene and cDNAs for the expression of α and β globin each driven by the H. polymorpha MOX promoter. After 40 generations in a chemostat it was found that the integrated vector had become amplified in the host strain. In some cases this led to an increase in LEU2 gene dosage, but a loss of globin expression cassettes. In other cases the globin gene dosage also increased. These changes coincided with an increase in rHbA production in the culture, which was reversed when the dilution rate was increased. Isolates from a chemostat culture producing elevated levels of rHbA were grown in fed-batch fermentations, resulting in higher productivities than when inoculated with the parent strain. The rHbA produced was purified and characterized. Oxygen binding studies and electrospray mass spectrometry showed that the rHbA had been processed and assembled correctly, and behaved as a fully functional co-operative tetramer.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/yea.320101206

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8

Digitale Medien

Zigzag Channels in the Structure of Sebaconitrile/UreaWe thank the donors of the Petroleum Research Fund, administered by the American Chemical Society (#24396-AC4), the Natural Sciences and Engineering and Research Council of Canada and the Alfred P. Sloan Foundation for financial support and Drs. John C. Huffman and Natsuko Cyr for their help with this work.Dedicted to the memory of Professor Margaret C. Etter (1994)

Hollingsworth, Mark D. ; Santarsiero, Bernard D. ; Harris, Kenneth D. M.

Weinheim : Wiley-Blackwell

Angewandte Chemie International Edition in English 33 (1994), S. 649-652

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ISSN: 0570-0833

Schlagwort(e): Chemistry ; General Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/anie.199406491

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9

Digitale Medien

Kinetics of formation of fibrin oligomers. III. Ligation kinetics concurrent with and subsequent to oligomer assembly (1984)

Bale, Marsha D. ; Janmey, Paul A. ; Ferry, John D. ; [weitere]

New York : Wiley-Blackwell

Biopolymers 23 (1984), S. 127-138

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ISSN: 0006-3525

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Human fibrinogen was treated with thrombin in the presence of fibrinoligase (Factor XIIIa) and calcium ion at pH 8.5, ionic strength 0.45, and the ensuing polymerization was interrupted at various time intervals (t) both before and after the clotting time (tc) by solubilization with a solution of sodium dodecyl sulfate and urea. Aliquots of the solubilized protein were subjected to gel electrophoresis on polyacrylamide gels after disulfide reduction by dithiothreitol and on agarose gels without reduction. The degree of γ-γ ligation was determined from the former. The latter provided the size distribution of ligated end-to-end sequences produced by splitting the ligated staggered overlapped oligomers down the middle, for degrees of polymerization, x, from 1 to 10. Addition of fibrinoligase (in which the activating thrombin had been inhibited by p-nitrophenyl-p′-guanidinobenzoate, NPGB) to Kabi fibrinogen showed the presence of small amounts of ligatable oligomers. Addition of fibrinoligase to a polymerizing mixture in which the action of thrombin had been stopped before clotting by NPGB produced the same distribution of ligated end-to-end sequences that was obtained when fibrinoligase was originally present, at least for reaction times up to 0.7 of the clotting time. The kinetics of γ-γ ligation by fibrinoligase acting on a polymerized mixture stabilized by NPGB were followed. The reaction was first order in the concentration of ligatable γ-γ junctions and the initial velocity was proportional to the enzyme concentration. The time evolution of size distribution of ligated end-to-end sequences agreed with a theory based on random ligation of ligatable junctions.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bip.360230110

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10

Digitale Medien

Triazine-based polymers, 2. Synthesis and characterization of polyethers (1994)

Braun, D. ; Most, D. ; Ziser, T.

Weinheim : Wiley-Blackwell

Angewandte Makromolekulare Chemie 221 (1994), S. 187-205

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ISSN: 0003-3146

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie , Physik

Beschreibung / Inhaltsverzeichnis: Polyether auf Triazinbasis können mittels phasentransferkatalysierter Polykondensation bei Raumtemperatur und Reaktionszeiten von 1 bis 3 h hergestellt werden. Von mehr als 20 Polyethern wurden Löslichkeiten, Glasübergangstemperaturen, thermogravimetrische Daten, Reißdehnungen, Reißfestigkeiten, Elastizitätsmoduli und Schlagzähigkeiten bestimmt. Von den Polymeren aus 2,4-Dichlor-6-diethylamino-s-triazin und Bisphenol A wurde zusätzlich der Kristallisationsgrad mittels Röntgenweitwinkelstreuung ermittelt. Das Inkrement des 1,3,5-Triazintriylrings wurde bestimmt, so daß die Glastemperaturen einiger Polyether nach van Krevelen berechnet werden konnten.

Notizen: Triazine-based polyethers can be synthesized by phase transfer catalysed interfacial polycondensation at room temperature and reaction times of about 1 to 3 h. In this way, more than 20 polyethers were synthesized and solubilities, glass transition temperatures, thermogravimetric data, elongations at break, tensil strengths, elasticity moduli and impact strengths were determined. In addition, the degree of crystallinity was investigated for the polymer obtained from of 2,4-dichloro-6-diethylamino-s-triazine and bisphenol A by wide angle X-ray diffraction. The increment of the 1,3,5-triazinetriyl ring was determined, which made it possible to calculate the glass transition temperatures of some polyethers according to van Krevelen.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/apmc.1994.052210116

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