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Depolarization of alfalfa root hair membrane potential by Rhizobium meliloti Nod factors (1992)

D. W. Ehrhardt ; E. M. Atkinson ; S. R. Long

American Association for the Advancement of Science (AAAS)

In: Science. 256(5059): 998-1000.

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Publication Date: 1992-05-15

Description: Although much is known about the bacterial genetics of early nodulation, little is known about the plant cell response. Alfalfa root hair cells were impaled with intracellular microelectrodes to measure a membrane potential depolarizing activity in Rhizobium meliloti cell-free filtrates, a plant response dependent on the bacterial nodulation genes. The depolarization was desensitized by repeated exposure to factors and was not observed in a representative nonlegume. A purified extracellular Nod factor, NodRm-IV(S), caused membrane potential depolarization at nanomolar concentrations. This rapid single-cell assay provides a tool for dissecting the mechanisms of host cell response in early nodulation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ehrhardt, D W -- Atkinson, E M -- Long, S R -- New York, N.Y. -- Science. 1992 May 15;256(5059):998-1000.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Stanford University, Stanford CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10744524" target="_blank"〉PubMed〈/a〉

Keywords: Lipopolysaccharides/*pharmacology ; Medicago sativa/cytology/*physiology ; Membrane Potentials/drug effects ; Plant Roots/cytology/drug effects/*physiology ; Sinorhizobium meliloti/physiology

Print ISSN: 0036-8075

Electronic ISSN: 1095-9203

Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics

Published by American Association for the Advancement of Science (AAAS)

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Malaria vaccines: multiple targets (1994)

R. S. Nussenzweig ; C. A. Long

American Association for the Advancement of Science (AAAS)

In: Science. 265(5177): 1381-3.

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Publication Date: 1994-09-02

Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nussenzweig, R S -- Long, C A -- AI21089/AI/NIAID NIH HHS/ -- AI35703-01/AI/NIAID NIH HHS/ -- AI37542-01/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1994 Sep 2;265(5177):1381-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medical and Molecular Parasitology, New York University Medical Center, NY 10010.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8073276" target="_blank"〉PubMed〈/a〉

Keywords: Animals ; Anopheles/parasitology ; Antigens, Protozoan/immunology ; Humans ; Insect Vectors/parasitology ; Malaria/*prevention & control/transmission ; *Malaria Vaccines/immunology ; Malaria, Falciparum/prevention & control/transmission ; Plasmodium/growth & development/immunology ; Plasmodium falciparum/growth & development/immunology ; T-Lymphocytes, Cytotoxic/immunology

Print ISSN: 0036-8075

Electronic ISSN: 1095-9203

Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics

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Natural selection and the origin of jingwei, a chimeric processed functional gene in Drosophila (1993)

M. Long ; C. H. Langley

American Association for the Advancement of Science (AAAS)

In: Science. 260(5104): 91-5.

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Publication Date: 1993-04-02

Description: The origin of new genes includes both the initial molecular events and subsequent population dynamics. A processed Drosophila alcohol dehydrogenase (Adh) gene, previously thought to be a pseudogene, provided an opportunity to examine the two phases of the origin of a new gene. The sequence of the processed Adh messenger RNA became part of a new functional gene by capturing several upstream exons and introns of an unrelated gene. This novel chimeric gene, jingwei, differs from its parent Adh gene in both its pattern of expression and rate of molecular evolution. Natural selection participated in the origin and subsequent evolution of this gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Long, M -- Langley, C H -- New York, N.Y. -- Science. 1993 Apr 2;260(5104):91-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section of Genetics, University of California, Davis 95616.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7682012" target="_blank"〉PubMed〈/a〉

Keywords: Alcohol Dehydrogenase/*genetics ; Animals ; Base Composition ; Base Sequence ; Biological Evolution ; Blotting, Northern ; *Chimera ; Drosophila/*genetics ; Introns ; Molecular Sequence Data ; Polymerase Chain Reaction ; RNA/analysis/chemistry ; RNA, Messenger/analysis/chemistry ; *Selection, Genetic ; Species Specificity

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Electronic ISSN: 1095-9203

Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics

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Naturally occurring variation in bristle number and DNA polymorphisms at the scabrous locus of Drosophila melanogaster (1994)

C. Lai ; R. F. Lyman ; A. D. Long ; [et al.]

American Association for the Advancement of Science (AAAS)

In: Science. 266(5191): 1697-702.

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Publication Date: 1994-12-09

Description: The association between quantitative genetic variation in bristle number and molecular variation at a candidate neurogenic locus, scabrous, was examined in Drosophila melanogaster. Approximately 32 percent of the genetic variation in abdominal bristle number (21 percent for sternopleural bristle number) among 47 second chromosomes from a natural population was correlated with DNA sequence polymorphisms at this locus. Several polymorphic sites associated with large phenotypic effects occurred at intermediate frequency. Quantitative genetic variation in natural populations caused by alleles that have large effects at a few loci and that segregate at intermediate frequencies conflicts with the classical infinitesimal model of the genetic basis of quantitative variation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lai, C -- Lyman, R F -- Long, A D -- Langley, C H -- Mackay, T F -- GM45146/GM/NIGMS NIH HHS/ -- GM45344/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1994 Dec 9;266(5191):1697-702.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Population Biology, University of California at Davis 95616.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7992053" target="_blank"〉PubMed〈/a〉

Keywords: Alleles ; Animals ; Base Sequence ; DNA/genetics ; *Drosophila Proteins ; Drosophila melanogaster/anatomy & histology/*genetics ; Female ; *Genes, Insect ; *Genetic Variation ; *Glycoproteins ; Haplotypes ; Linkage Disequilibrium ; Male ; Molecular Sequence Data ; Phenotype ; *Polymorphism, Genetic ; Proteins/*genetics ; Restriction Mapping ; Sense Organs/anatomy & histology

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Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics

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5

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Women in biomedicine: encouragement (1994)

S. R. Long ; V. Zakian

American Association for the Advancement of Science (AAAS)

In: Science. 263(5152): 1357-8.

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Publication Date: 1994-03-11

Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Long, S R -- Zakian, V -- New York, N.Y. -- Science. 1994 Mar 11;263(5152):1357-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8128213" target="_blank"〉PubMed〈/a〉

Keywords: Female ; Humans ; *Research ; *Women, Working

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Electronic ISSN: 1095-9203

Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics

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