Publication Date:
2016-07-02
Description:
The present research was undertaken to study the probiotic characteristics of Pichia kudriavzevii isolated from frozen idli batter. PCR amplification with 18S rRNA primers confirmed Pichia kudriavzevii , a xylose utilizing probiotic strain. It was resistant to physiological concentrations of bile salts, pepsin and pancreatic enzyme. It also showed efficient auto-aggregation as well as co-aggregation ability with 4 commercial probiotic yeasts and exhibited good hydrophobicity in xylene and toluene. The strain inhibited the growth of 13 enteropathogens and showed commensal relationship with 4 commercial probiotic yeast and bacteria. Moreover, it was resistant to 30 antibiotics with different modes of action. The yeast exhibited thermo-tolerance up to 95 °C for 2 h. The cell-free supernatants were also found to be heat-stable indicating the presence of thermo-stable secondary metabolites. Hence, it could be exploited as starter culture, co-culture or probiotic in the preparation of fermented products or incorporated in heat-able foods as well.
Print ISSN:
0749-503X
Electronic ISSN:
1097-0061
Topics:
Biology
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