Publikationsdatum:
2018
Beschreibung:
We developed a microfluidic device within which we generated a concentration gradient of Wnt3a and cultured neuroblastoma SH‐SY5Y cells. We found that a centriole associated with higher concentration of ODF2 protein was significantly localized to the higher concentration side, with over 2.5 × 10−3 nm·μm−1 of the Wnt3a gradient.
Proper determination of the cell division axis is essential during development. Wnt3a is a known regulator of the cell division axis; however, the sensitivity of cells to Wnt3a signalling and its role in determining the cell division axis have not been measured to date. To address this gap, we took advantage of the asymmetric distribution of outer dense fibre 2 (ODF2/cenexin) proteins on centrosomes in dividing cells. To precisely quantify the sensitivity of cells to Wnt3a signalling, we developed a microfluidic cell culture device, which can produce a quantitative gradient of signalling molecules. We confirmed that mitotic SH‐SY5Y neuroblastoma cells could detect a 2.5 ~ 5 × 10−3 nm·μm−1 Wnt3a concentration gradient and demonstrated that this gradient is sufficient to affect the determination of the pole‐to‐pole axis of cell division during the later stages of mitosis.
Digitale ISSN:
2211-5463
Thema:
Biologie
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