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Low temperature hydrogenation of iron nanoparticles on graphene (2014)

Keisuke Takahashi; Yongming Wang; Shotaro Chiba; Yuki Nakagawa; Shigehito Isobe; Somei Ohnuki

Springer Nature

In: Scientific Reports

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Publication Date: 2014-04-09

Description: Hydrogenation of iron nanoparticles was performed both computationally and experimentally where previously chemically-bonded iron hydride is considered to be unachievable under ordinary conditions. Density functional theory (DFT) calculations predict that hydrogenated iron nanoparticles are stabilized on a single-layer graphene/Cu substrate. Experimentally, iron nanoparticles were deposited onto a graphene/Cu substrate by vacuum deposition. Hydrogenation was done at 1atm of hydrogen gas and under liquid nitrogen. Mass spectrometry peak confirmed the hydrogen release from hydrogenated iron nanoparticles while a scanning transmission electron microscopy is used in order to link a geometrical shape of iron hydride nanoparticles between experimental and theoretical treatments. The hydrogenated iron nanoparticles were successfully synthesized where hydrogenated iron nanoparticles are stable under ordinary conditions. Scientific Reports 4 doi: 10.1038/srep04598

Electronic ISSN: 2045-2322

Topics: Natural Sciences in General

Published by Springer Nature

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Efficient and cost effective production of active-form human PKB using silkworm larvae (2014)

Ryoko Maesaki; Ryosuke Satoh; Masato Taoka; Teppei Kanaba; Tsunaki Asano; Chiharu Fujita; Toshinobu Fujiwara; Yutaka Ito; Toshiaki Isobe; Toshio Hakoshima; Katsumi Maenaka; Masaki Mishima

Springer Nature

In: Scientific Reports

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Publication Date: 2014-08-16

Description: Protein kinase B (PKB) also known as Akt is involved in many signal transduction pathways. As alterations of the PKB pathway are found in a number of human malignancies, PKB is considered an important drug target for cancer therapy. However, production of sufficient amounts of active PKB for biochemical and structural studies is very costly because of the necessity of using a higher organism expression system to obtain phosphorylated PKB. Here, we report efficient production of active PKBα using the BmNPV bacmid expression system with silkworm larvae. Following direct injection of bacmid DNA, recombinant PKBα protein was highly expressed in the fat bodies of larvae, and could be purified using a GST-tag and then cleaved. A final yield of approximately 1 mg PKBα/20 larvae was recorded. Kinase assays showed that the recombinant PKBα possessed high phosphorylation activity. We further confirmed phosphorylation on the activation loop by mass spectrometric analysis. Our results indicate that the silkworm expression system is of value for preparation of active-form PKBα with phosphorylation on the activation loop. This efficient production of the active protein will facilitate further biochemical and structural studies and stimulate subsequent drug development. Scientific Reports 4 doi: 10.1038/srep06016

Electronic ISSN: 2045-2322

Topics: Natural Sciences in General

Published by Springer Nature

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Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy (2014)

Masamitsu Oshima; Kaoru Inoue; Kei Nakajima; Tetsuhiko Tachikawa; Hiromichi Yamazaki; Tomohide Isobe; Ayaka Sugawara; Miho Ogawa; Chie Tanaka; Masahiro Saito; Shohei Kasugai; Teruko Takano-Yamamoto; Takashi Inoue; Katsunari Tezuka; Takuo Kuboki; Akira Yamaguchi; Takashi Tsuji

Springer Nature

In: Scientific Reports

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Publication Date: 2014-08-14

Description: Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy. Scientific Reports 4 doi: 10.1038/srep06044

Electronic ISSN: 2045-2322

Topics: Natural Sciences in General

Published by Springer Nature

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