ALBERT

Beschreibung: Hyperpolarization-activated, cyclic nucleotide-gated cation (HCN) channels are critical regulators of neuronal excitability, but less is known about their possible roles in synaptic plasticity and memory circuits. Here, we characterized the HCN gene organization, channel properties, distribution, and involvement in associative and nonassociative forms of learning in Aplysia californica. Aplysia has only one HCN gene, which codes for a channel that has many similarities to the mammalian HCN channel. The cloned acHCN gene was expressed in Xenopus oocytes, which displayed a hyperpolarization-induced inward current that was enhanced by cGMP as well as cAMP. Similarly to its homologs in other animals, acHCN is permeable to K+ and Na+ ions, and is selectively blocked by Cs+ and ZD7288. We found that acHCN is predominantly expressed in inter- and motor neurons, including LFS siphon motor neurons, and therefore tested whether HCN channels are involved in simple forms of learning of the siphon-withdrawal reflex in a semiintact preparation. ZD7288 (100 μM) significantly reduced an associative form of learning (classical conditioning) but had no effect on two nonassociative forms of learning (intermediate-term sensitization and unpaired training) or baseline responses. The HCN current is enhanced by nitric oxide (NO), which may explain the postsynaptic role of NO during conditioning. HCN current in turn enhances the NMDA-like current in the motor neurons, suggesting that HCN channels contribute to conditioning through this pathway.

Beschreibung: Hyperpolarization-activated, cyclic nucleotide-gated cation (HCN) channels are critical regulators of neuronal excitability, but less is known about their possible roles in synaptic plasticity and memory circuits. Here, we characterized the HCN gene organization, channel properties, distribution, and involvement in associative and nonassociative forms of learning in Aplysia californica. Aplysia has...

Beschreibung: We report on a genome-wide scan for introgression between the house mouse (Mus musculus domesticus) and the Algerian mouse (Mus spretus), using samples from the ranges of sympatry and allopatry in Africa and Europe. Our analysis reveals wide variability in introgression signatures along the genomes, as well as across the...

Beschreibung: Current drought monitoring and early warning systems use different indicators for monitoring drought conditions and apply different indicator thresholds and rules for assigning drought intensity classes or issue warnings or alerts. Nevertheless, there is little knowledge on the meaning of different hydro-meteorologic indicators for impact occurrence on the ground. To date, there have been very few attempts to systematically characterize the indicator–impact relationship owing to sparse and patchy data on drought impacts. The newly established European Drought Impact report Inventory (EDII) offers the possibility to investigate this linkage. The aim of this study was to explore the link between hydro-meteorologic indicators and drought impacts for the case study area Germany and thus to test the potential of qualitative impact data for evaluating the performance of drought indicators. As drought indicators two climatological drought indices – the Standardized Precipitation Index (SPI) and the Standardized Precipitation Evapotranspiration Index (SPEI) – as well as streamflow and groundwater level percentiles were selected. Linkage was assessed though data visualization, extraction of indicator values concurrent with impact onset, and correlation analysis between monthly time series of indicator and impact data at the federal state level, and between spatial patterns for selected drought events. The analysis clearly revealed a significant moderate to strong correlation for some states and drought events allowing for an intercomparison of the performance of different drought indicators. Important findings were strongest correlation for intermediate accumulation periods of SPI and SPEI, a slightly better performance of SPEI versus SPI, and a similar performance of streamflow percentiles to SPI in many cases. Apart from these commonalities, the analysis also exposed differences among federal states and drought events, suggesting that the linkage is time variant and region specific to some degree. Concerning "thresholds" for drought impact onset, i.e. indicator values concurrent with past impact onsets, we found that no single "best" threshold value can be identified but impacts occur within a range of indicator values. Nevertheless, the median of the threshold distributions showed differences between northern/northeastern versus southern/southwestern federal states, and among drought events. While the findings strongly depend on data and may change with a growing number of EDII entries in the future, this study clearly demonstrates the feasibility of evaluating hydro-meteorologic variables with text-based impact reports and highlights the value of impact reporting as a tool for monitoring drought conditions.