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  • 1
    Electronic Resource
    Electronic Resource
    Unequal recombination between tandemly repeated chloroplast ribosomal operons (1985)
    Springer
    Current genetics 10 (1985), S. 229-238 
    Details
    ISSN: 1432-0983
    Keywords: Chloroplast ; Euglena ; Ribosomal-operon ; Unequal-recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Modifications of the chloroplast genome have been investigated in 35 mutants ofEuglena gracilis which have antibiotic-resistant chloroplasts. Whereas each molecule of wild-type chloroplast DNA has three tandemly repeated ribosomal operons (rrn), endonuclease restriction and electron microscopy studies demonstrate that the number of chloroplastrrn is frequently modified in the mutants: only one operon is remaining in 16 of the 35 mutants; among the 14 streptomycinresistant strains, nine have one operon, one has 2 operons, and another one has 4 operons. Modifications of the number of chloroplastrrn may be explained by unequal recombinations between theserrn, arising either between the operons of the same molecule or between the operons of two molecules. These recombinations might occur during a repair process following the mutagenic treatment. Some loci for antibiotic resistance might be located at the recombination point on therrn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00798753
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  • 2
    Electronic Resource
    Electronic Resource
    Modifications of chloroplast DNA during streptomycin induced mutagenesis in Euglena gracilis (1982)
    Springer
    Current genetics 5 (1982), S. 9-15 
    Details
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Mutagenesis ; Ribosomal cistron ; Amplification ; Euglena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The evolution of chloroplast DNA was analysed during streptomycin induced mutagenesis in Euglena gracilis strain bacillaris and strain Z. In addition to a massive reduction of the cellular level of chloroplast DNA, several structural modifications have been observed in early stages of mutagenesis but they are generally eliminated during the later stages. The ribosomal cistrons are regularly rearranged: two of the three tandemly arranged cistrons occuring in wild type chloroplast DNA decrease while the third one is relatively more conserved and amplified during mutagenesis and in bleached mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445734
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative estimations of chloroplast DNA in bleached mutants of Euglena gracilis (1982)
    Springer
    Current genetics 6 (1982), S. 111-117 
    Details
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Bleached mutants ; Ribosomal cistrons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The level of chloroplast DNA has been estimated in bleached mutants of Euglena by the increase of the renaturation rate of a radioactive chloroplast DNA probe in response to the addition of total mutant DNA. Two classes of bleached mutants differ from each other by their level of chloroplast DNA: - a few bleached mutants contain chloroplast DNA in amounts similar to those of wild type strains; all sequences of the wild type chloroplast DNA seem to be present but in non stoechiometric proportions. - most bleached mutants have about 100 to 1,000 times less chloroplast DNA than wild type cells. In these mutants chloroplast DNA sequences form two frequency classes: - one class has about 5 to 12% of the complexity of the wild type genome; these sequences are reiterated from 30 to 160 times per cell and hybridize with wild type ribosomal cistrons. They are expressed as chloroplast ribosomal RNA in all bleached mutants analyzed so far. - the second class shows at least 40% of the complexity of the wild type genome; these sequences are present in 2 to 7 copies per cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435209
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  • 4
    Electronic Resource
    Electronic Resource
    Comparative studies of chloroplastic and nuclear DNA repair abilities after ultraviolet irradiation of Euglena gracilis (1980)
    Springer
    Molecular genetics and genomics 178 (1980), S. 567-572 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies of nuclear and chloroplastic-DNA repair after ultraviolet irradiation of Euglena gracilis show that photoreactivation is very efficient at both the nuclear and chloroplastic level. Liquid-holding or split-dose experiments and treatment with caffeine reveal, furthermore, that dark-repair is very efficient in nuclear DNA but not in chloroplastic DNA (ctDNA). The possibility of a chloroplastic dark-repair of restricted efficiency is discussed. Determination of chloroplastic DNA content by reassociation kinetics indicates that an important degradation follows UV irradiation during liquid holding in the dark.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337862
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  • 5
    Electronic Resource
    Electronic Resource
    Production of plastidial antibiotic-resistant mutants by ultraviolet irradiation of Euglena gracilis (1982)
    Springer
    Molecular genetics and genomics 185 (1982), S. 184-185 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultraviolet-irradiation of Euglena gracilis drastically increases the proportion of green colonies resistant to chloroplast-inhibiting antibiotics. Some green colonies persist at high UV doses. These results might indicate the occurrence in the chloroplastic DNA of repair processes, of which one could be error-prone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333812
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  • 6
    Electronic Resource
    Electronic Resource
    Somatic segregation and rate of greening after ultraviolet irradiation of Euglena gracilis (1977)
    Springer
    Molecular genetics and genomics 155 (1977), S. 123-129 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. During multiplication of irradiated cells, a segregation may take place between bleached cells, whose progeny is unable to green, and green ones. Some of the green cells give progenies exclusively made of green cells; the progeny of others is partly composed of bleached cells. 2. If one assumes that greening results from the activity of functional units endowed with genetic continuity (Plastidial Segregating Units=PSU), segregation of these units seems to occur according to a model involving random sorting out during the three first divisions. During the following divisions, functional units seem to multiply faster than those impaired by irradiation. 3. The greening rate of colonies issued from irradiated cells seems to be conditioned mostly by the number of functional PSU remaining in the mother cell of the colony.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393150
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  • 7
    Electronic Resource
    Electronic Resource
    Analyses of quantitative trait loci associated with resistance to shape Sclerotinia sclerotiorum in sunflowers (shape Helianthus annuus L.) using molecular markers (1998)
    Springer
    Molecular breeding 4 (1998), S. 215-226 
    Details
    ISSN: 1572-9788
    Keywords: Helianthus annuus ; QTL ; resistance ; RFLP ; Sclerotinia scerotorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Restriction fragment length polymorphism and isoenzyme markers were used to investigate quantitative trait loci involved in sunflower resistance to mycelial extension of Sclerotinia sclerotiorum on leaves and capitula. Seed weight, oil content and flowering data were also evaluated. Four quantitative trait loci were demonstrated for leaf resistance and two for capitulum resistance. One of these zones appears involved in resistance to both types of S. sclerotiorum attack while the others appear specific for resistance of one part of the plant. Two quantitative trait loci were detected for seed weight, three for oil content and three for flowering date. Individual quantitative trait loci explained 9% to 48% of the phenotypic variability, confirming the polygenic basis of the quantitative traits studied. Overall, the quantitative trait loci explain 60% of the genetic variation for leaf resistance and 38% for capitulum resistance to S. sclerotiorum. One linkage group is particularly interesting since it includes quantitative trait loci for all the five quantitative traits measured. Hypotheses for linkage versus pleiotropy and consequences of all the results in resistance breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009694626992
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  • 8
    Electronic Resource
    Electronic Resource
    Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.) (1996)
    Springer
    Euphytica 91 (1996), S. 225-228 
    Details
    ISSN: 1573-5060
    Keywords: bulked segregant analysis ; Helianthus annuus ; linkage ; Plasmopara halstedii ; resistance genes ; RFLP ; sunflower ; downy mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Pl6 locus in the inbred sunflower (Helianthus annuus L.) line HA335 giving resistance to French races of downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni. was localized by molecular techniques. A bulked segregant analysis was made on the F2 progeny from a cross between this line and H52, a downy mildew susceptible line. The resistance gene in HA335 was found to have the same linked RFLP marker loci as those determined for Pl1 (resistance to race 1 in the line RHA266) on linkage group 1 of the consensus RFLP map of the cultivated sunflower. Pl1 and Pl6 thus appear either to be allelic or closely linked. The implications for sunflower breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021074
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