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  • Articles  (5)
  • Articles: DFG German National Licenses  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Leprosy vaccine (1994)
    [s.l.] : Nature Publishing Group
    Nature 369 (1994), S. 436-436 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR - According to Bloom et al.1 the two leprosy vaccine strains, ICRC and M.w., showed identical bands in the RFLP analysis at only 2 or 3 points. But this is an understatement. The authors used two enzymes (Pstl and BstElT) and two probes, 3.6 kb Mycobacterium leprae 65 kD antigen and 65 kD M. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/369436b0
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  • 2
    Electronic Resource
    Electronic Resource
    Cell Regeneration in Protein Deficiency (1967)
    [s.l.] : Nature Publishing Group
    Nature 216 (1967), S. 499-500 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Twenty-six male albino Wistar rats, 120-150 g, were divided into two equal groups; one group was fed a protein deficient diet containing 1 per cent casein given freely for 5-6 weeks; the other, control group, was fed a diet containing adequate protein (16 per cent casein) for an equal period. Each ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/216499a0
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of enhancing factor gene and its localization in mouse tissues (1991)
    Springer
    Histochemistry and cell biology 96 (1991), S. 367-370 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Enhancing factor (EF), a 14 kDa protein, isolated from mouse small intestines, has been reported from this laboratory. Based on our earlier studies EF has been implicated in cell proliferation. Preliminary immunohistochemical studies have shown EF to be localized in the Paneth cells of small intestines. In this paper we report the tissue distribution of EF using conditions optimized for immunohistochemical staining. In addition, the data are supported by northern blot analysis using a nick translated cDNA probe specific for EF. The results indicate that EF gene is actively transcribed mainly in the intestines. The chief source of synthesis of EF appears to be the Paneth cells located at the base of the crypts of Lieberkühn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271358
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  • 4
    Electronic Resource
    Electronic Resource
    Ontogeny of enhancing factor in mouse intestines and skin (1991)
    Springer
    Histochemistry and cell biology 96 (1991), S. 371-374 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Enhancing Factor (EF) is a 14 kDa protein isolated from mouse small intestines, which enhances the binding of 125I-EGF to A431 cells. This observation as well as our earlier in vitro studies have indicated that EF is a modulator of EGF. In adult mice, localization of EF by immunohistochemistry shows it is present pre-dominantly in the Paneth cells of the small intestines and to a lesser extent in the stomach and colon. This study of the ontogeny of EF shows that the appearance of the protein coincides with the appearance of mature Paneth cells. In new born mouse skin EF is localized in the hair follicles in the first hair cycle from day 2 to day 8. It is however absent in the adult skin. Thus EF is associated with tissues which have a high growth rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271359
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  • 5
    Electronic Resource
    Electronic Resource
    Further studies on the enhancing factor and its possible mechanism of action (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 127 (1986), S. 183-188 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this study the nature of binding of enhancing factor (EF) and its mode of action are examined. EF binds to A431 cells through its own receptor, which is distinct from the receptor for epidermal growth factor (EGF). EF binds to the cell membrane and in turn provides a binding site for EGF. Data analyzed from Scatchard plots show that prior treatment of formalin-fixed A431 cells with EF for 30 minutes results in an increase in the number of binding sites for 125I-EGF. 3H-Thymidine incorporation studies, using the EGF-receptorless cell line NR-6, indicate that neither EF nor EGF alone stimulates the cells to synthesise DNA, but when both are added together the cells show 3H-thymidine incorporation. The role of EF may be to trap EGF and make it available to the cells through its own receptors even in the absence of EGF receptors. EF also induces anchorage-independent growth of normal fibroblasts in soft agar only in the presence of EGF.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041270122
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