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  • 1
    Electronic Resource
    Electronic Resource
    Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 11 (1994), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The cspA is a gene of Escherichia coli, whose expression is specifically induced at low temperatures to a level of 13% of total protein synthesis. The CspA protein consisting of 70 amino add residues has high sequence similarity with eukaryotic Y-box DNA-binding proteins. We found two independent clones from the Kohara miniset phage collection, which hybridized with a DNA fragment containing cspA. DNA sequencing of these clones confirmed that the two genes are highly homologous to cspA. One designated cspB is mapped at 35 min on the E. coli chromosome and encodes a 71-residue protein with 79% identity to CspA, while the other, cspC, is mapped at 40 min and encodes a 69-residue protein with 70% identity. In addition, a DNA sequence upstream of the clpA gene at 19 mm published elsewhere contains an open reading frame for a 74-residue protein with 45% identity to CspA. All csp genes were fused in the coding regions with the lacZ gene, and the expression of β-galactosidase was examined for these hybrid genes upon cold shock. A similar cold-shock induction to cspA was observed for cspB but not cspC and cspD. These results Indicate that E. coli has a family of the cspA gene, some of which are induced by cold shock.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1994.tb00361.x
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of the enzyme activity of an extracellular metalloprotease (VMC) from Vibrio mimicus and its C-terminal deletions (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 223 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To investigate the enzymatic properties of Vibrio mimicus metalloprotease, the mature metalloprotease gene (vmc) was overexpressed in Escherichia coli and the recombinant protein (rVMC61) was purified by metal affinity chromatography. rVMC61 showed maximum activity at about 37°C, pH 8. The purified rVMC61 was very specific toward collagen substrates, such as gelatin, type I, II, and III collagens and synthetic peptides (Cbz-GPLGP and Cbz-GPGGPA). But it did not show degrading activity toward other biological proteins including lysozyme, lactoferrin and bovine serum albumin. rVMC61 also showed cytotoxicity against CHSE-214 fish cells. To examine the role of the C-terminal region of rVMC61, the 3′ end of the metalloprotease gene (vmc) was digested serially with exonuclease III. The truncated vmc derivatives encoding 57–42 kDa of the protease were isolated and overexpressed in E. coli. The collagenase activities of truncated proteins were investigated using gelatin as substrate. Deletion of 100 amino acids from the C-terminus resulted in loss of gelatin degrading activity. However, deletion of 67 amino acids from the C-terminus did not affect its gelatin degrading activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00401-4
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  • 3
    Electronic Resource
    Electronic Resource
    Microstructures and Growth Characteristics of Self-Assembled InAs/GaAs Quantum Dots Investigated by Transmission Electron Microscopy (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 26-28 (Oct. 2007), p. 1207-1210 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The microstructure and strain characteristics of self-assembled InAs/GaAs quantum dots(QDs) were studied by using transmission electron microscopy. Compressive strain was induced touncapped QDs from GaAs substrate and the misfit strain largely increased after the deposition ofGaAs cap layer. Tensile strain outside QD was extended along the vertical growth direction; up to 15nm above the wetting layer. Vertically nonaligned and aligned stacked QDs were grown by adjustingthe thickness of GaAs spacer layers. The QDs with a lens-shaped morphology were formed in theearly stage of growth, and their apex was flattened by the out-diffusion of In atoms upon GaAscapping. However, aligned QDs maintained their lens-shaped structure with round apex after capping.It is believed that their apex did not flatten because the chemical potential gradient of In was relativelylow due to the adjacent InAs QD layers
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.26-28.1207.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    A review of data mining techniques (2001)
    Bingley : Emerald
    Industrial management & data systems 101 (2001), S. 41-46 
    Details
    ISSN: 0263-5577
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science , Economics
    Notes: Terabytes of data are generated everyday in many organizations. To extract hidden predictive information from large volumes of data, data mining (DM) techniques are needed. Organizations are starting to realize the importance of data mining in their strategic planning and successful application of DM techniques can be an enormous payoff for the organizations. This paper discusses the requirements and challenges of DM, and describes major DM techniques such as statistics, artificial intelligence, decision tree approach, genetic algorithm, and visualization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/02635570110365989
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  • 5
    Electronic Resource
    Electronic Resource
    Algalytic activity of α-mannosidase on harmful marine microalgae (2000)
    Springer
    Journal of applied phycology 12 (2000), S. 191-193 
    Details
    ISSN: 1573-5176
    Keywords: algalytic activity ; α-mannosidase ; Cochlodinium polykrikoides ; dinoflagellate ; red tide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract α-D mannose is present on the surface of thetoxic dinoflagellate Cochlodinium polykrikoides.When exposed to the enzyme α-mannosidase, livecells of C. polykrikoides lost motility, roundedup, swelled gradually, and then lysed. The enzymedemonstrated a wide spectrum of lytic activity towardthe harmful algal species of the family Gymnodiniales,Alexandrium tamarense and Eutreptiellagymnastica, but showed less effect on useful feedalgae such as Chaetoceros calcitrans, Dunaliella salina, Isochrysis galbana, and Navicula pelliculosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008124313633
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  • 6
    Electronic Resource
    Electronic Resource
    The cps gene cluster of Salmonella strain LT2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 173-180 
    Details
    ISSN: 1617-4623
    Keywords: GC ratio ; cps ; rfb ; Phosphomannomutase ; Mannose-1-phosphate guanyltransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report the presence in Salmonella enterica strain LT2 (serovar thyphimurium) of duplicate genes for two steps in the synthesis of GDP-mannose. The previously known genes, rfbK (phosphomannomutase) and rfbM (mannose-l-phosphate guanyltransferase), are part of the gene cluster for the O antigen. The two new genes, cpsB and cpsG, respectively, are thought to be part of the gene cluster for the M antigen capsular polysaccharide, present in many Enterobacteriaceae. The two genes have been sequenced and have a GC content of 0.61, suggesting an origin outside of Salmonella. Comparison of the inferred protein sequences for cpsB and rfbM shows 57% identity of amino acids whereas for cpsG and rfbK there is only 19% identity. It is suggested that the greater divergence between cpsG and rfbK may be due to a period of accelerated evolution, perhaps precipitated by transfer of the genes from another species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259668
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  • 7
    Electronic Resource
    Electronic Resource
    A Novel Technique for the Effective Production of Short Peptide Analogs from Concatameric Short Peptide Multimers (2000)
    Springer
    Molecules and cells 10 (2000), S. 236-240 
    Details
    ISSN: 0219-1032
    Keywords: Cleavable Linker Peptide ; Concatameric Peptide Multimers ; Peptide Analogs ; Tandemly Repeated DNA Cassette
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We designed a basic unit of the modified chicken gonadotropin releasing hormone II (cGnRH-II) peptide containing a trypsin cleavable linker peptide at both ends of the original peptide. We made a synthetic DNA coding for the modified cGnRH-II peptide with asymmetric and complementary cohesive ends of linker nucleotides. A tandemly repeated DNA cassette for the expression of concatameric short peptide multimers was constructed by ligating the basic units. The expressed peptide multimers were purified and subject to amino-terminal sequence analysis, which displayed the amino acid sequences expected from the designed nucleotides of the expression cassette. The monomeric cGnRH-II peptide analogs were generated after trypsin digestion. The present results showed that the technique developed for the production of the concatameric peptide multimers with cleavable linker peptides can be generally applicable to the production of short peptide analogs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s10059-000-0236-9
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