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  • Articles  (7)
  • Articles: DFG German National Licenses  (7)
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  • Articles  (7)
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  • 1
    Electronic Resource
    Electronic Resource
    High resolution G-banded chromosomes of the mouse (1987)
    Springer
    Chromosoma 95 (1987), S. 350-358 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract High resolution G-banded mouse chromosomes were prepared using an actinomycin D and acridine orange pretreatment protocol, resulting in late prophase mouse chromosomes which reveal over twice the number of bands as compared with mid metaphase. These elongated chromosomes, described here in detail and used to construct a precise schematic representation of the late prophase banding patterns, should be generally useful in high resolution mouse chromosome analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293182
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  • 2
    Electronic Resource
    Electronic Resource
    Variation in genomic alu repeat density as a basis for rapid construction of low resolution physical maps of human chromosomes (1992)
    Springer
    Chromosoma 101 (1992), S. 349-357 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human DNA restriction fragments containing high numbers of Alu repeat sequences can be preferentially detected in the presence of other human DNA restriction fragments in DNA from human:rodent somatic cell hybrids when the DNA is fragmented with enzymes that cleave mammalian DNA infrequently. This ability to lower the observed human DNA complexity allowed us to develop an approach to order rapidly somatic hybrid cell lines retaining overlapping human genomic domains. The ordering process also generates a relative physical map of the human fragments detected with Alu probe DNA. This process can generate physical mapping information for human genomic domains as large as an entire chromosome (100,000 kb). The strategy is demonstrated by ordering Alu-detected NotI fragments in a panel of mouse:human hybrid cells that span the entire long arm of human chromosome 17.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00346014
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  • 3
    Electronic Resource
    Electronic Resource
    Subunit structure of chromosomes in mitotic nuclei of Physarum polycephalum (1976)
    Springer
    Chromosoma 57 (1976), S. 95-102 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have investigated the subunit structure of mitotic chromosomes of the acellular slime mould Physarum polycephalum, using the nuclease susceptibility of isolated mitotic nuclei as a probe. A characteristic pattern of DNA digestion products is obtained, containing approximately integral multiples of a basic 140 base pair DNA segment that resembles very closely the pattern in G2 phase nuclei of Physarum and of calf lymphocyte nuclei. These results demonstrate that during the process of chromosome condensation there is no alteration at the primary level of chromatin structure that is responsible for the characteristic DNA digestion pattern.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292952
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  • 4
    Electronic Resource
    Electronic Resource
    Evidence for a four micron replication unit in CHO cells (1976)
    Springer
    Chromosoma 57 (1976), S. 341-350 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract CHO cells in culture were synchronized by mitotic selection, allowed to reattach to plastic flasks, and reach S phase in the presence of fluorodeoxyuridine at concentrations known to completely block the synthesis of thymidylate. The cells were released from the block with 3H-thymidine for pulses of 4, 8, 12, 24 and 40 min and DNA fiber autoradiographs prepared. An analysis of the spacing between origins of replication indicates that sites are available at intervals of about 4 μm along most of the DNA. Chain growth proceeds at about 1,000 nucleotides per minute and some of the closely situated sites become continuous, labeled segments after 8–12 min. However, unlabeled segments are still present between the replicated segments after 40 min. The data may be interpreted as evidence for regularly spaced initiation sites which are available in CHO cells, even though only one in 10–15 of these may be utilized for initiation each cycle under normal growth conditions in these cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332159
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  • 5
    Electronic Resource
    Electronic Resource
    Structure of human chromosomes visualized at the electron microscopic level (1981)
    Springer
    Chromosoma 82 (1981), S. 55-64 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A newly developed technique allows cytological (light microscope level) chromosome preparations to be examined at the electron microscopic level. Ultrathin (50 nm) sections of highly condensed Hela cell metaphase chromosomes show the characteristic mitotic chromosome morphology. In addition a fibrous network (presumably chromosome fibers) can be seen within them. Fibers appear to be gathered at foci along each chromatid. Treatment of chromosomes with trypsin in a trypsin/G-banding procedure reduces the amount of staining material at the electron microscopic level and results in more prominent foci. Thicker (100 nm) sections of less condensed chromosomes prepared from human lymphocytes display a banding pattern similar to G-banding, even without pretreatment with proteases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285749
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  • 6
    Electronic Resource
    Electronic Resource
    Evidence for chromosomal replicons as units of sister chromatid exchanges (1989)
    Springer
    Chromosoma 98 (1989), S. 69-76 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Chromosomal replicons have been described as the cytological counterpart of DNA replicon clusters and have previously been studied in vitro using premature chromosome condensation-sister chromatid differentiation (PCC-SCD) techniques. Chromosomal replicons are visualized as small SCD segments in S-phase cells, and measurement of these segments can provide estimates of relative chromosomal replicon size corresponding to DNA replicon clusters functioning coordinately in S-phase. Current hypotheses of sister chromatid exchange (SCE) formation postulate that sites of SCE induction are associated with active replicons or replicon clusters. We have applied the PCC-SCD technique to in vivo studies of mouse bone marrow cells that have been treated with cyclophosphamide (CP) for two cell cycles. We have been able to visualize chromosomal replicons, as well as SCEs which have been induced in vivo by CP treatment, simultaneously in the same cells. Chromosomal replicons visualized as small SCD segments were measured in PCC cells classified at early or late S-phase based on SCD segment size prevalence. Early S-phase (E/S) PCC cells contained 90% of the SCD segments measured clustered in a segment size range of 0.1 to 0.8 μm with a peak value around 0.3 to 0.6 μm regardless of CP treatment. As the cells progressed through S-phase, late S-phase (L/S) PCC cells were characterized by the appearance of larger SCD segments and even whole SCD chromosomes in addition to small SCD segments. A concentration of units around 0.4 to 1.0 μm was found for L/S SCD segment size distributions regardless of CP treatment with an apparent bimodal profile. Our in vivo data support the existence of a subunit organization of chromosomal replication with a basic functional unit being 0.3 to 0.6 μm in size. In addition, we have found that this chromosomal unit of replication or “chromosomal replicon” does not seem to be functionally perturbed by the mutagen CP. We also found that small SCD segments of 0.4 to 0.7 μm in length were involved in the formation of an SCE, suggesting that both spontaneous and CP-induced SCEs occur between chromosomal replicons. These findings provide direct cytogenetic evidence to support a replicon cluster/chromosomal replicon model for SCE formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293337
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  • 7
    Electronic Resource
    Electronic Resource
    Localization of the mouse lissencephaly-1 gene to mouse chromosome 11B3, in close proximity to D11Mit65 (1995)
    Springer
    Somatic cell and molecular genetics 21 (1995), S. 345-349 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Lissencephaly is a human brain malformation manifested by a smooth cerebral surface and severe mental retardation. Some of the patients have been shown to have deletions in chromosome 17p13.3, and recently, LIS-1 has been proposed to be the disease-associated gene. We have now mapped the mouse homolog of LIS-1 to mouse chromosome 11B3 by using fluorescence in situ hybridization to metaphase chromosomes. The analysis of yeast artificial chromosome clones placed Lis-1 in close proximity to the microsatellite marker D11Mit65.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02257469
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