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1

Digitale Medien

Production of indole alkaloids by surface immobilized C. roseus cells (1990)

Archambault, J. ; Volesky, B. ; Kurz, W. G. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 35 (1990), S. 660-667

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A technique was developed to surface immobilize plant cells and was scaled up in laboratory size bioreactors. This technique was shown not to hinder the biosynthetic potential of Catharanthus roseus immobilized cells and to induce a partial release (300 μg/L) of serpentine into the culture medium contrary to suspension cultured cells. The release pattern seemed to follow the biosynthesis trends of the product. This release mechanism could be stimulated by a factor of 10 within 2 h by increasing the pH of the culture from 5.0 to 5.5.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260350703

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2

Digitale Medien

Surface immobilization of plant cells (1989)

Archambault, J. ; Volesky, B. ; Kurz, W. G. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 33 (1989), S. 293-299

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A novel technique has been developed to immobilize plant cells. The cells are deposited on a surface of manmade fibrous material that provides for strong binding of the plant tissue biomass growing in the submerged culture. The immobilized plant cells remain fully viable. Relatively uniform biomass loadings of up to 20 mg d.w. plant cells/cm2 support material have been attained. All plant cells from the inoculum suspension became attached within the first 24-48 h depending on the support matrix configuration and hydraulic culture conditions. The advantages and scale-up potential of this technique are discussed and compared to other culturing modes.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260330307

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3

Digitale Medien

Development of bioreactors for the culture of surface immobilized plant cells (1990)

Archambault, J. ; Volesky, B. ; Kurz, W. G. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 35 (1990), S. 702-711

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: The scaleup of the technique of plant cell surface immobilization was performed successfully in specifically designed laboratory size bioreactors. The immobilizing matrix was formed into a vertically wound spiral providing for a high immobilizing area-to-volume ratio (0.8-1.2 cm-1). A modified airlift and a mechanically stirred vessel delivered a best bioreactor performance characterized by low biomass frothing and highly efficient plant cell attachment and retention (≥96%). The growth of Catharanthus roseus cells investigated in these bioreactors was found not to be mass transfer limited. It required mild mixing and aeration levels (kLa ∼ 10-15 h-1). The biomass formation pattern of surface immobilized plant cells generally exhibited a linear growth phase followed by a stationary phase characterized by the presence of residual carbohydrates in the medium, contrary to suspension cultures. This behavior was found to depend on the plant cell type and/or line cultured, as well as on the inoculum age. The space restriction and unidirectional growth of the SIPC biofilm combined with the limited availability of essential intracellular nutrients rapidly accumulated from the medium by the stationary phase inoculated plant cells all likely contributed to the culture behavior.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260350707

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4

Digitale Medien

Somatostatinergic nerves in the cervical spinal cord of the monkey (1979)

Burnweit, C. ; Forssmann, W. G.

Springer

Cell & tissue research 200 (1979), S. 83-90

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ISSN: 1432-0878

Schlagwort(e): Spinal cord ; Tupaia ; Somatostatin ; Immunohistochemistry

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Somatostatinergic nerves in the spinal cord of the monkey were investigated utilizing immunohistochemistry with various antibodies against synthetic somatostatin. In contrast to earlier investigations, it is shown that somatostatinergic nerve endings occur in most of the areas of the grey matter of the spinal cord. The somatostatinergic axons are, however, characteristically distributed in three main regions: (1) Densely-packed endings are seen in lamina II of the substantia gelatinosa, forming a crescent-shaped pattern in the columna dorsalis. Somatostatin immunoreactivity is also seen in lamina I and in the Lissauer tract. (2) A fine network of fibers is observed around the central canal; the endings are concentrated on special cell bodies. Some single perikarya are also stained in this region. (3) A loose network of single fibers is found ending on perikarya of the columna lateralis or ventralis. The perikarya of the nerve axons, with the exception of those terminating in the columna dorsalis, have as yet not been identified. In order to better understand the somatostatinergic system of the spinal cord, these newly-detected somatostatinergic nerves must be studied and their exact pathways analyzed.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00236889

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5

Digitale Medien

Insulin-, glucagon-, and somatostatin-immunoreactive endocrine cells in the equine pancreas (1976)

Helmstaedter, V. ; Feurle, G. E. ; Forssmann, W. G.

Springer

Cell & tissue research 172 (1976), S. 447-454

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ISSN: 1432-0878

Schlagwort(e): Pancreas ; Horse ; Immunohistochemistry ; Insulin ; Glucagon ; Somatostatin

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Equine pancreas was investigated with immunohistochemical methods to study the distribution of endocrine cells immunoreactive to antiinsulin, anti-glucagon, and anti-somatostatin. A-cells demonstrable by antiglucagon are located in the center of Langerhans islets and frequently in the duct epithelium. Few A-cells are seen associated to acini. Anti-insulin reactive B-cells form a large zone around the center of the Langerhans islets in which some B-cells lie between exocrine cells and others, although few, are located in the duct epithelium. D-cells stained with anti-somatostatin serum form a discontinuous outermost zone around the Langerhans islets. In some islets the D-cells are also observed among the B-cells or between the border of A- and B-cells. Single D-cells are seen in the duct epithelium or between acinar cells. In younger horses, endocrine cells are more frequently associated in bulges of the duct system. The histotopographic relation between these endocrine cell types is discussed with respect to its functional significance.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00220331

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6

Digitale Medien

Met-enkephalin-like immunoreactivity in Merkel cells (1979)

Hartschuh, W. ; Weihe, E. ; Büchler, M. ; [weitere]

Springer

Cell & tissue research 201 (1979), S. 343-348

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ISSN: 1432-0878

Schlagwort(e): Merkel cell ; Sinus hair follicle ; Rat skin ; Enkephalin ; Immunohistochemistry

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The Merkel cells from sinus hair follicles of rats were investigated by immunohistochemistry using different antisera against neuropeptides and gastroenteropancreatic (GEP)-hormones. For the first time it has been demonstrated that Merkel cells exhibit an immunoreactivity towards metenkephalin (methionine-enkephalin). The met-enkephalin immunoreactivity was restricted to Merkel cells and was not found in associated nerve axons or terminals. Denervation of Merkel cells did not affect the met-enkephalin immunoreactivity. Antisera against leu-enkephalin (leucine-enkephalin) and other polypeptides did not produce an immunoreaction. The demonstration of met-enkephalin-like immunoreactivity supports the concept that the Merkel cell is a member of the paraneuronal system and a potential neuroreceptor cell.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00236994

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7

Digitale Medien

Substance P-immunoreactive neurons in the brainstem of the cat related to cardiovascular centers (1985)

Triepel, J. ; Weindl, A. ; Kiemle, I. ; [weitere]

Springer

Cell & tissue research 241 (1985), S. 31-41

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ISSN: 1432-0878

Schlagwort(e): Substance P ; Cardiovascular system ; Central nervous system ; Immunohistochemistry ; Mapping ; Cat

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The distribution of substance P-immunoreactivity (SP-IR) in the brainstem and spinal cord of normal and colchicine-pretreated cats was analysed using the peroxidase-antiperoxidase (PAP) technique. Numerous SP-IR fibers are present in the nucleus solitarius, nucleus dorsalis nervi vagi and nucleus spinalis nervi trigemini, various parts of the formatio reticularis, substantia grisea centralis mesencephali, locus coeruleus and nucleus parabrachialis. SP-IR perikarya occur in the substantiae gelatinosa and intermedia of the spinal cord, the nucleus spinalis nervi trigemini-pars caudalis, the nucleus dorsalis nervi vagi, and the nucleus solitarius, as well as in the adjacent formatio reticularis and the medullary nuclei of the raphe. In addition, SP-IR cell bodies are located in the nuclei raphe magnus and incertus, ventral and dorsal to the nucleus tegmentalis dorsalis (Gudden), nucleus raphe dorsalis, substantia grisea centralis mensencephali, locus coeruleus, nucleus parabrachialis and colliculus superior. The results indicate that SP-IR neurons may be involved in the regulation of cardiovascular functions both at the central and peripheral level. A peripheral afferent portion seems to terminate in the nucleus solitarius and an efferent part is postulated to originate from the nucleus dorsalis nervi vagi and from the area of the nuclei retroambiguus, ambiguus and retrofacialis.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00214623

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8

Digitale Medien

The auricular myocardiocytes of the heart constitute an endocrine organ characterization of a porcine cardiac peptide hormone, cardiodilatin-126 (1984)

Forssmann, W. G. ; Birr, C. ; Carlquist, M. ; [weitere]

Springer

Cell & tissue research 238 (1984), S. 425-430

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ISSN: 1432-0878

Schlagwort(e): Heart, atrium ; Myoendocrine cells ; Cardiodilatin ; Peptide hormone ; Immunohistochemistry ; Pig

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary A peptide hormone was extracted from the porcine right atrium following a bioassay for differential vaso-relaxant effects on smooth muscle strips from aorta and renal and inferior mesenteric arteries. The isolation procedure included several steps of gel-permeation and ion-exchange chromatography, and high performance liquid chromatography. During the isolation procedure, other peptides of smaller molecular weight were also found, which, in relation to cardiodilatin-126 (CDD-126), are shorter at their N-terminal. Among these, CDD-88 has also been isolated and characterizied, and has been established as a prominent member of the cardiac hormone family. The N-terminal and C-terminal segments of the 126 amino acid-containing molecule were synthesized and used to raise region-specific antibodies. The natural peptide was then localized within myoendocrine cells of the right atrium where specific atrial granules are located. Renal effects of cardiodilation were studied in conscious dogs and showed strong diuretic and natriuretic activities. According to our functional studies, cardiodilatin-126 and cardiodilatin-88 possess qualities of a significant hormone family regarding the regulation of extracellular fluid volume and blood pressure.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00219856

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9

Digitale Medien

Maintaining perpetual synchrony in HeLa S3 culture (1974)

Thilly, W. G. ; Nowak, T. S. ; Wogan, G. N.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 16 (1974), S. 149-156

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: HeLa S3 cells in suspension culture are resynchronized in each successive generation by exposure to 0.25mM thymidine. The resynchronization approach appears to be superior to single- and double-block techniques because the period of effective inhibition of DNA synthesis is significantly shortened. Gram quantities of highly synchronous viable cells have been produced on a daily basis.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260160111

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10

Digitale Medien

Maintenance of perpetual synchrony in HeLa suspension cultures: Question of unbalanced growth (1975)

Thilly, W. G. ; Arkin, D. I. ; Nowak, T. S. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 17 (1975), S. 703-712

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Unbalanced growth has been studied in HeLa cell cultures maintained in perpetual division synchrony by periodic inhibition of DNA synthesis. Observation of eight independent cellular parameters indicates that once synchrony has been established the degree of unbalanced growth in later cycles is not as great as is observed with the conventional “double-block” technique. This significant diminution of unbalanced growth appears to be related to the significantly shorter periods of inhibition of DNA synthesis used in the resynchronization process.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260170507

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