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  • Articles  (3)
  • Articles: DFG German National Licenses  (3)
  • Numerical Methods and Modeling  (2)
  • Keywords: keratinase; gene cloning; gene expression; Bacilli  (1)
  • 1995-1999  (3)
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  • Articles  (3)
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  • Articles: DFG German National Licenses  (3)
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  • 1995-1999  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the Bacillus licheniformis PWD-1 keratinase gene in B. subtilis (1997)
    Springer
    Journal of industrial microbiology and biotechnology 19 (1997), S. 134-138 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: keratinase; gene cloning; gene expression; Bacilli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kerA gene which encodes the enzyme keratinase was isolated from the feather-degrading bacterium Bacillus licheniformis PWD-1. The entire gene, including pre-, pro- and mature protein regions, was cloned with Pker, its own promoter, P43, the vegetative growth promoter, or the combination of P43-Pker into plasmid pUB18. Transformation of the protease-deficient strain B. subtilis DB104 with these plasmids generated transformant strains FDB-3, FDB-108 and FDB-29 respectively. All transformants expressed active keratinase in both feather and LB media, in contrast to PWD-1, in which kerA was repressed when grown in LB medium. With P43-Pker upstream of kerA, FDB-29 displayed the highest activity in feather medium. Production of keratinase in PWD-1 and transformants was further characterized when glucose or casamino acids were supplemented into the feather medium. These studies help understand the regulation of kerA expression and, in the long run, can help strain development and medium conditioning for the production of this industrially important keratinase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900440
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of Jordan chains by extended matrices (1998)
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 14 (1998), S. 879-893 
    Details
    ISSN: 1069-8299
    Keywords: derogatory eigenproblems ; Jordan blocks ; Jordan chains ; Segre characteristic ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The major obstacle to determination of the Jordan chains for a highly degenerated eigenproblem is that the triangular combinations of the principal vectors in a Jordan chain are also principal vectors and the linear combinations of the eigenvectors of all Jordan blocks associated with the same eigenvalue are also eigenvectors. These indeterminate constants will hide the Jordan block structure and make the analysis very difficult. We propose an extended matrix method to find the Jordan chains and eliminate the indeterminate constants so that the Jordan block structure can be computed sequentially. An example with the Segre characteristic [(321)11] is given. © 1998 John Wiley & Sons, Ltd.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 3
    Electronic Resource
    Electronic Resource
    Finite element solution for the continuum traffic equilibrium problems (1998)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 43 (1998), S. 1253-1273 
    Details
    ISSN: 0029-5981
    Keywords: finite element method ; continuum modelling ; traffic equilibrium ; user-optimal pattern ; fixed demand ; variable demand ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper, we consider a city with a highly compact Central Business District (CBD), and the commuters’ destinations from the CBD are dispersed over the whole city. The street network is approximated as a continuum and commuters’ movements in the city are measured by the flow intensity, and the local travel cost depends on the location and the traffic flow intensity. We extend the continuum user equilibrium problem to deal with the case of variable demand, in which the traffic demand from any destination in the city to the CBD is assumed to be a function of both the destination location and the total travel cost to the CBD. An equivalent mathematical model is formulated and proved to satisfy the user equilibrium conditions, which is then solved by a finite element solution algorithm. Numerical examples are given to illustrate the effectiveness of the proposed method. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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