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  • Articles  (2)
  • Data
  • Articles: DFG German National Licenses  (2)
  • 5-Bromo-4-chloro-3-indolyl-N  (1)
  • Caribbean pine  (1)
  • 1995-1999  (2)
  • 1965-1969
  • 1945-1949
Collection
  • Articles  (2)
  • Data
Source
  • Articles: DFG German National Licenses  (2)
Publisher
Years
  • 1995-1999  (2)
  • 1965-1969
  • 1945-1949
Year
  • 1
    ISSN: 1432-1890
    Keywords: Key words Ectomycorrhizae ; Chitinolytic activities ; Localization ; 4-Methylumbelliferyl substrates [4-MU-(GlcNAc)1 ; 4] ; 5-Bromo-4-chloro-3-indolyl-N ; acetyl-β-d-glucosaminide (X-GlcNAc)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Localization of chitinolytic activities in Fagus sylvatica (beech) mycorrhizas was examined using a range of fluorogenic 4-methylumbelliferyl [4-MU-(GlcNAc)1–4] substrates in order to distinguish between exochitinase, endochitinase and β-N–acetylglucosaminidase activities. The validity of the technique was confirmed using onion epidermis cells. In the beech mycorrhiza, endochitinase activity was not detectable above background fluorescence. Exochitinase activity was detected in the fungal sheath and the Hartig net. β-N–Acetylglucosaminidase activity was also mainly associated with the fungal sheath and Hartig net. Individual fungal hyphae extending from these structures also showed substantial β-N–acetylglucosaminidase activity. The cortical cell walls of the host in the Hartig net region also fluoresced brightly. The localization of β-N–acetylglucosaminidase activity was confirmed using a chromogenic histochemical reagent, 5-bromo-4-chloro-3-indolyl-N–acetyl-β-d-glucosaminide (X-GlcNAc).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5095
    Keywords: slash pine ; Caribbean pine ; freeze hardiness ; general hybrid combining ability ; Pinus elliottii ; Pinus caribaea var. bahamensis ; var. hondurensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Six pine taxa were compared for freeze hardiness following 2 whole plant freeze tests carried out in early and late winter 1993–94. Pure species included slash pine (Pinus elliottii var. elliottii Engelman) (PEE), Caribbean pine (Pinus caribaea Morelet) var. bahamensis (PCB), and var. hondurensis (PCH). The same six slash pine parents served as the female parents of the pure slash pine taxon and were control pollinated to create the F1 hybrids of slash pine × Caribbean pine var. bahamensis (F1B), the F1 of slash pine × Caribbean pine var. hondurensis (F1H), and the backcross of slash pine × (slash pine × var. hondurensis (B1H). Four traits related to adaptation (bud set, foliage/stem damage, survival) were compared. Significant differences existing among the three species at all temperatures for both freeze tests. The 3 hybrid taxa were intermediate to parental taxa for all measured traits and only at extreme temperatures was there an indication of hybrid vigor. However, inspection of foliage damage curves to estimate temperatures at which 50 percent damage occurs indicated that the hybrids deviated from the mid parent value toward the more freeze susceptible parent. Discriminant functions which included foliage damage and survival traits provided the best segregation of taxa groupings and the combining of canonical variable 1 for these discriminant functions placed hybrids intermediate to parental taxa. Parent × taxa interaction was significant, suggesting that within these taxa, family to family variation occurred, but different maternal parents were associated with the freeze hardly families in different taxa.
    Type of Medium: Electronic Resource
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