ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
From 12 to 24 h after the opening of daylily flowers (Hemerocallis hybrid cv. Stella d'Oro), the petals begin to degrade and the protein levels of soluble, microsomal- and plastid-enriched fractions decrease by 50%, on a per petal basis. To help determine some of the components for the cell death program in daylily petals, we studied the mechanisms that regulate this loss of protein. Enzyme activities capable of digesting native daylily protein, gelatin, and azocasein markedly increase after flower opening, and their appearance is inhibited by the translation inhibitor, cycloheximide. Protein hydrolysis in vitro is prevented by inhibitors of cysteine, serine and metalloproteinases. Immunoblots using antibodies to ubiquitin pathway enzymes indicate that the ubiquitin system is not senescence specific. However, ion leakage is delayed by two inhibitors of the 26S proteasome. We propose that programmed cell death in daylily petals may involve the increase in activity of at least three classes of proteinases, and discuss the possibility that these proteinases may operate in concert with the ubiquitin pathway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1034/j.1399-3054.1998.1040323.x
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