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1

Digitale Medien

Effects of mycorrhizal infection, soil phosphorus availability and fruit production on the male function in two cultivars of Lycopersicon esculentum (2001)

Poulton, J. L. ; Koide, R. T. ; Stephenson, A. G.

Oxford, UK : Blackwell Science Ltd

Plant, cell & environment 24 (2001), S. 0

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ISSN: 1365-3040

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: The effects of mycorrhizal infection, soil P availability and fruit production on the male function of reproduction were examined in two cultivars of tomato (Lycopersicon esculentum Mill.). Tomato plants were grown in a greenhouse under three treatment combinations: non-mycorrhizal, low P (NMPO); non-mycorrhizal, high P (NMP3); and mycorrhizal, low P (MPO). In addition, all treatment combinations were grown both with and without fruit. Fruit production decreased final leaf biomass, flower production and in vitro pollen tube growth rates, often reducing the beneficial effects of increased P uptake. Thus, fruit production diverted resources from subsequent vegetative growth, flower production and pollen development. As the growing season progressed, mean pollen production per flower and in vitro germination and tube growth decreased. Mycorrhizal infection and high soil P conditions increased final leaf biomass, flower production, mean pollen production per flower (in one cultivar) and in vitro pollen tube growth rates. Thus, mycorrhizal infection and high soil P conditions increased pollen quantity and quality, thereby enhancing fitness through the male function. Similar trends in these treatments suggested that mycorrhizal effects on the male function were largely the result of improved P acquisition.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1046/j.0016-8025.2001.00735.x

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2

Digitale Medien

Resistance to quinclorac and ALS-inhibitor herbicides in Galium spurium is conferred by two distinct genes (2004)

Van Eerd, L L ; McLean, M D ; Stephenson, G R ; [weitere]

Oxford, UK : Blackwell Science Ltd

Weed research 44 (2004), S. 0

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ISSN: 1365-3180

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft

Notizen: Classical Mendelian experiments were conducted to determine the genetics and inheritance of quinclorac and acetolactate synthase (ALS)-inhibitor resistance in a biotype of Galium spurium. Plants were screened with the formulated product of either quinclorac or the ALS-inhibitor, thifensulfuron, at the field dose of 125 or 6 g active ingredient (a.i.) ha−1 respectively. Segregation in the F2 generation indicated that quinclorac resistance was a single, recessive nuclear trait, based on a 1 : 3 segregation ratio [resistant : susceptible (R : S)]. Resistance to ALS inhibitors was due to a single, dominant nuclear trait, segregating in the F2 generation in a 3 : 1 ratio (R : S). The genetic models were confirmed by herbicide screens of F1 and backcrosses between the F1 and the S parent. F2 plants that survived quinclorac treatment set seed and the resulting F3 progeny were screened with either herbicide. Quinclorac-treated F3 plants segregated in a 1 : 0 ratio (R : S), hence F2 progenitors were homozygous for quinclorac resistance. In contrast, F3 progeny segregated into three ratios: 1 : 0, 3 : 1 and 0 : 1 (R : S) in response to ALS-inhibitor treatment. This segregation pattern indicates that their F2 parents were either homozygous or heterozygous for ALS-inhibitor resistance. Therefore, there were clearly two distinct resistance mechanisms encoded by two genes that were not tightly linked as demonstrated by segregation patterns of the F3.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1365-3180.2004.00408.x

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3

Digitale Medien

Interaction surface of the Spo0A response regulator with the Spo0E phosphatase (2002)

Stephenson, Sophie J. ; Perego, Marta

Oxford, UK : Blackwell Science Ltd

Molecular microbiology 44 (2002), S. 0

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ISSN: 1365-2958

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie , Medizin

Notizen: Spo0A~P is the essential response regulator and transcription factor for sporulation initiation in Bacillus subtilis. The phosphorylation level of Spo0A in the cell is determined by the sensor kinase activity of the phosphorelay, donating phosphoryl groups, and the antagonistic effects of dephosphorylation mediated by the Rap and Spo0E families of phosphatases. In this study, spo0A mutations were generated that encoded proteins less sensitive to the activity of Spo0E than the wild-type protein. The Spo0A substitutions N12K, P60S, L62P and F88L are surface exposed and localize to the same face of the molecule as the active site and in its close proximity on the β1–α1, β3–α3 and β4–α4 loops. The corresponding surface in the Spo0F response regulator was shown previously to be involved in the interaction with the RapB phosphatase, as well as the KinA histidine kinase and the Spo0B phosphotransferase. Thus, residues occupying the same position (N12:Q12, F88:Y84) and the same loops in Spo0A or Spo0F are involved in the interaction with the structurally unrelated Spo0E and RapB phosphatases, respectively, in addition to kinases and phosphotransferase. The specificity in phosphatase target recognition must be the result of side-chain variability within the response regulators and the interactions they promote. The residues involved in Spo0E interaction are identical in all Spo0A orthologues from spore-forming Bacilli encoding Spo0E phosphatases.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1046/j.1365-2958.2002.02974.x

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4

Unbekannt

When Disaster Threatens ... How to Head Off a Catastrophe in Public Relations (1983)

Stephenson, D. R.

Saranac Lake, N.Y., etc. : Periodicals Archive Online (PAO)

Management Review. 72:11 (1983:Nov.) 51

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ISSN: 0025-1895

Thema: Wirtschaftswissenschaften

URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:7504-1983-072-11-000007

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5

Digitale Medien

Darstellung und Eigenschaften neuer perhalogenierter 1,3-Dithietan-S-oxide (1983)

Eschwey, Manfred ; Sundermeyer, Wolfgang ; Stephenson, David S.

Weinheim : Wiley-Blackwell

Berichte der deutschen chemischen Gesellschaft 116 (1983), S. 1623-1630

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ISSN: 0009-2940

Schlagwort(e): Chemistry ; Inorganic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Beschreibung / Inhaltsverzeichnis: Synthesis and Properties of New Perhalogenated 1,3-Dithietane S-OxidesNew perchlorinated and perfluorinated 1,3-dithietanes (4, 6, 7, 12, 14, 15), which are partially oxidized at the sulfur atoms, are described. Pyrolysis of the 1,3-dioxides 7 and 15 leads to the sulfoxides 8 and 16, X2C = S = O (X = Cl, F). respectively. Two new oxidizing agents are used: trifluoromethane- and nonafluorobutanepersulfonic acid.

Notizen: Die neuen perchlorierten und perfluorierten, am Schwefel partiell oxidierten 1,3-Dithietane 4, 6, 7, 12, 14 und 15 werden beschrieben. Pyrolysen der 1,3-Dioxide 7 und 15 führen zu den Sulfoxiden 8 bzw. 16, X2C = S = O (X = Cl, F). Zwei neue Oxidationsmittel werden angewendet: Trifluormethan- und Nonafluorbutanpersulfonsäure.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/cber.19831160437

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6

Digitale Medien

Proline excretion by Escherichia coli K12A preliminary report of this research was made at the Fourth International Symposium on the Genetics of Industrial Microoganisms in Kyoto, Japan, June, 1982. (1984)

Rancourt, Derrick E. ; Stephenson, James T. ; Vickell, Gregg A. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 26 (1984), S. 74-80

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Proline excretion from proline overproducing strains of E. coli K12 has been studied as a model chemical production system. We have isolated proline overproducing mutants of E. coli and have shown that uncontrolled synthesis is not sufficient to cause excretion of this amino acid. An episomal mutation causing proline over production has been introduced into a series of otherwise isogenic strains that bear well defined, chromosomal lesions affecting the active uptake and catabolism of L-proline. A syntropism test reveals that L-proline is excreted by overproducing strains only if transport and/or catabolism are impaired. Dansyl derivatization and chromatographic analysis of culture supernatants shows that proline is the only amino acid excreted. Batch cultures of an excreting strain in an amino acid production medium yield culture supernatants containing 1 g proline/L, whereas no proline is detectable in supernatants derived from cultures of an overproducing strain with normal transport and catabolic activities. These data reveal that genetic lesions eliminating active uptake can be used to specifically enhance metabolite excretion.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260260114

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7

Digitale Medien

Proline excretion by Escherichia coli K12 (1984)

Rancourt, D. E. ; Stephenson, J. T. ; Vickell, G. A. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 26 (1984), S. 1140-1140

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260260924

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8

Digitale Medien

Monoclonal antibodies to feline sarcoma virus gag and fes gene translational products (1983)

Veronese, Fulvia ; Kelloff, Gary J. ; Reynolds, Fred H. ; [weitere]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 21 (1983), S. 9-18

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ISSN: 0730-2312

Schlagwort(e): feline sarcoma virus proteins ; v-fes ; v-fms ; tyrosine ; monoclonal antibodies ; tyrosine phosphorylation ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: A series of hybridomas have been isolated which produce monoclonal antibodies directed against polyprotein gene products of the Gardner, Snyder-Theilen, and McDonough strains of FeSV. Within these are representatives of several immunoglobulin classes including IgG1, IgG2a, IgG2b, IgG2c, and IgM. Antibody produced by one hybridoma recognizes immunologic determinants localized within an FeLV gag gene structural component (p15) common to polyproteins encoded by all three FeSV isolates whereas antibody produced by a second is specific for p30 determinants unique to P170gag-fms. Additional hybridomas secrete antibody directed against v-fes-encoded determinants common to the Gardner and Snyder-Theilen FeSV-encoded polyproteins. GA P110gag-fes and ST P85gag-fes immunoprecipitated by antibody directed against p15 exhibit tyrosine-specific protein kinase activity but lack such activity when precipitated by antibody specific for their acquired sequence (v-fes) components.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcb.240210103

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9

Digitale Medien

The human c-abl oncogene in the philadelphia translocation (1984)

Groffen, John ; Stephenson, John R. ; Heisterkamp, Nora ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 121 (1984), S. 179-191

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041210421

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10

Digitale Medien

Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)

Garner, D. L. ; Johnson, L. A. ; Lake, S. ; [weitere]

New York, NY : Wiley-Blackwell

Gamete Research 10 (1984), S. 339-351

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ISSN: 0148-7280

Schlagwort(e): spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrd.1120100402

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