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  • Articles  (6)
  • Other Sources
  • Articles: DFG German National Licenses  (6)
  • Ajmalicine  (2)
  • Herbivory  (2)
  • autophosphorylation  (2)
  • Springer  (6)
  • AGU (American Geophysical Union)
  • Elsevier
  • 2015-2019
  • 2010-2014
  • 1990-1994  (6)
  • 1960-1964
  • Biology  (6)
  • Political Science
  • Physics
  • Architecture, Civil Engineering, Surveying
Collection
  • Articles  (6)
  • Other Sources
Source
  • Articles: DFG German National Licenses  (6)
Keywords
Publisher
  • Springer  (6)
  • AGU (American Geophysical Union)
  • Elsevier
Years
  • 2015-2019
  • 2010-2014
  • 1990-1994  (6)
  • 1960-1964
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Demography of Adenostoma fasciculatum after fires of different intensities in southern California chaparral (1993)
    Springer
    Oecologia 96 (1993), S. 95-101 
    Details
    ISSN: 1432-1939
    Keywords: Fire intensity ; Adenostoma fasciculatum ; Lignotuber ; Resprouting ; Herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the population structure of the lignotuberous resprouter Adenostoma fasciculatum were investigated by experimentally burning at various intensities an old stand in southern California chaparral. Survival after fire, time of resprouting, herbivory, and survival after resprouting were monitored for more than 1 year after the fire. Greater fire intensity increased plant mortality and the size of plants that died as a consequence of the fire. Time from the fire until resprouting increased with increasing fire intensity and was related to plant size: the larger the plants the earlier they resprouted. Post-resprouting mortality also increased with fire intensity and was related to time of resprouting. Herbivory increased with fire intensity and the size of plants affected by it changed with the intensity of the fire. Fire intensity had profound direct and indirect effects on the population structure of Adenostoma fasciculatum. Plant size strongly determined the direct and indirect lethal effects of fire.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318035
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Fire intensity and herbivory effects on postfire resprouting of Adenostoma fasciculatum in southern California chaparral (1991)
    Springer
    Oecologia 85 (1991), S. 429-433 
    Details
    ISSN: 1432-1939
    Keywords: Adenostoma fasciculatum ; California chaparral ; Fire intensity ; Herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Resprouting is the main regeneration mechanism after fire in Mediterranean-type ecosystems. Herbivores play an important role in controlling postfire seedling establishment, but their influence on regeneration by resprouting is less well known. To study the effects of fire intensity on resprouting of Adenostoma fasciculatum in southern California chaparral, and its interaction with herbivory, we conducted an experimental burn at three levels of fire intensity. We found that increasing fire intensity increased plant mortality, reduced the number of resprouts per plant, and delayed the time of resprouting. Herbivory increased with fire intensity, and was related to the time of resprouting. Plants resprouting later in the season and out of synchrony with the main flush were attacked more readily by herbivores. Post-resprouting mortality also increased with fire intensity and was significantly associated with herbivory in the higher fire intensity plots. Fire intensity effects on chaparral regeneration by resprouting may be farreaching through effects on the population structure, resprout production, and growth of Adenostoma fasciculatum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320621
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Different phosphorylation behaviour of regulatory subunit isoforms of type 11 cAMP-dependent protein kinase from bovine heart (1990)
    Springer
    Molecular and cellular biochemistry 96 (1990), S. 25-33 
    Details
    ISSN: 1573-4919
    Keywords: cAMP-dependent protein kinase ; autophosphorylation ; limited proteolysis ; isoforms of regulatory subunits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Two forms of the regulatory subunit of the type II cAMP-dependent protein kinase (RII55 and RII52) were identified from bovine heart by gel electrophoretic behaviour. After autophosphorylation the RII55 isoform migrated more slowly (RII55/57) while the migration of RII52 isoform did not shift. Both isoforms showed different affinity for cAMP. The RII55/57 isoform was eluted from a cAMP-agarose column at 10 mM cAMP at low ionic strenght whereas the RII52 isoform required cAMP, plus 2 M NaCl. Partial proteolysis, using trypsin or formic acid, of autophosphorylated regulatory subunit isoforms resulted in different cleavage pattern as determined by peptide mapping. However, the V8125I-peptides patterns of both isoforms are quite similar. Incubation of partially purified holoenzyme with 10 nM [γ-32P]ATP (low ATP concentration) yielded a single band of Mr = 57,000 which corresponds to the RII55/57 isoform. The incubation, however, at 20 µM [γ-32P]ATP yielded two phosphobands corresponding to both RII55/57 and RII52 isoforms. The phosphorylation of RII52 took place with a lower efficiency and was more sensitive to the cAMP than the corresponding phosphorylation of the RII55/57.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228450
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  • 4
    Electronic Resource
    Electronic Resource
    Dipyridamole stimulates types II cAMP-dependent protein kinase in vitro (1992)
    Springer
    Molecular and cellular biochemistry 109 (1992), S. 9-15 
    Details
    ISSN: 1573-4919
    Keywords: cAMP-dependent protein kinase ; dipyridamole ; lipid metabolism ; autophosphorylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Dipyridamole activates in vitro type II CAMP-dependent protein kinase. This agent stimulates the autophosphorylation of the regulatory subunit in the presence of CAMP but not so in the absence of the cyclic nucleotide. The activation was also observed with exogenous substrates such as casein, histone 2A and MAP 2. This stimulation did not seem to be related to the cAMP binding to the R II subunit of the enzyme. Competition binding experiments showed that dipyridamole does not compete with adenosine for the A1 receptor. The results suggest that the reported regulatory properties of dipyridamole on lipid metabolism (González-Nicolás et al. Int J Biochem 21: 883–888, 1989) might be mediated through a direct action — an activation — on the catalytic subunit of a cAMP-dependent protein kinase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230868
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  • 5
    Electronic Resource
    Electronic Resource
    Ajmalicine production by cell cultures of Catharanthus roseus: from shake flask to bioreactor (1994)
    Springer
    Plant cell, tissue and organ culture 38 (1994), S. 85-91 
    Details
    ISSN: 1573-5044
    Keywords: Ajmalicine ; bioreactor ; Catharanthus roseus ; growth model ; scale-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The productivity of a cell culture for the production of a secondary metabolite is defined by three factors: specific growth rate, specific product formation rate, and biomass concentration during production. The effect of scaling-up from shake flask to bioreactor on growth and production and the effect of increasing the biomass concentration were investigated for the production of ajmalicine by Catharanthus roseus cell suspensions. Growth of biomass was not affected by the type of culture vessel. Growth, carbohydrate storage, glucose and oxygen consumption, and the carbon dioxide production could be predicted rather well by a structured model with the internal phosphate and the external glucose concentration as the controlling factors. The production of ajmalicine on production medium in a shake flask was not reproduced in a bioreactor. The production could be restored by creating a gas regime in the bioreactor comparable to that in a shake flask. Increasing the biomass concentration both in a shake flask and in a stirred fermenter decreased the ajmalicine production rate. This effect could be removed partly by controlling the oxygen concentration in the more dense culture at 85% air saturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033865
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  • 6
    Electronic Resource
    Electronic Resource
    Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures (1994)
    Springer
    Plant cell, tissue and organ culture 38 (1994), S. 273-279 
    Details
    ISSN: 1573-5044
    Keywords: Ajmalicine ; alkaloids ; catharanthine ; Catharanthus roseus ; hairy roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two year old, transformed root cultures of Catharanthus roseus accumulate ajmalicine and catharanthine (0.57 and 0.36 mg g-1 DW, or 7.0 and 3.0 mg l-1, respectively). Changes in the concentration of the medium components, as well as the addition of hydrolytic enzymes and biotic elicitors, were used as strategies to increase these alkaloid yields. Regarding the components of the medium, the results obtained, when sucrose was raised from 3 to 4.5%, are noteworthy. The nitrogen source induced differential responses in the individual alkaloid yields. No net change in the alkaloid content was observed either with changes in the concentration of vitamins or macro-and micronutrients. Though the root culture only shows a limited response to elicitors, Aspergillus treatment and the use of macerozyme increased the accumulation of ajmalicine selectively, while the addition of methyl jasmonate increased the yield of both alkaloids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033887
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