ISSN:
1432-072X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Two isoenzymes of alcohol dehydrogenase (adh I and adh II) from Saccharomyces cheresiensis have been differentiated by thermal treatment of the crude extracts. The effect of pH on the stability and the K m for ethanol are different for the two isoenzymes. The proportions in which they are present depend on the carbon source used by the yeast: adh I is the major component in cells grown on glucose, and adh II in those grown on ethanol. Cells grown on glucose plus ethanol show high levels of both isoenzymes, indicating that the synthesis of adh I is subjected to nutritional induction by glucose, and that of adh II by ethanol. The physiological roles of the two isoenzymes are discussed in relation with the nutritional characteristics of S. cheresiensis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00412435
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