Publication Date:
1989-03-31
Description:
An immunohistochemical study was conducted to detect the temporal infection sequence of Autographa californica M nuclear polyhedrosis virus in Trichoplusia ni larvae. Staining patterns indicated that the initial infection occurred in the midgut, simultaneously in columnar epithelial and regenerative cells, but that subsequently this tissue recovered. A major envelope glycoprotein stained in a polar fashion when it was expressed in columnar epithelial cells, but not when expressed in other cells types. Systemic infection was mediated by free virus for some tissues whereas infected hemocytes appeared to spread virus to other tissues by an unknown mechanism. A cell to cell spread within several tissues was detected. These results have important implications for baculoviruses engineered for improving their pesticide potential.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Keddie, B A -- Aponte, G W -- Volkman, L E -- AM 38310-01/AM/NIADDK NIH HHS/ -- New York, N.Y. -- Science. 1989 Mar 31;243(4899):1728-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Entomology, University of California, Berkeley.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2648574" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal
;
Antigens, Viral/analysis
;
Capsid/analysis
;
Epithelium/analysis/microbiology
;
Hemolymph/microbiology
;
Immunoenzyme Techniques
;
Immunohistochemistry
;
Insect Viruses/immunology/isolation & purification/*physiology
;
Larva/microbiology
;
Lepidoptera/*microbiology
;
Membrane Glycoproteins/analysis
;
Tissue Distribution
;
Viral Proteins/analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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