ALBERT

Description: Our growing awareness of the microbial world’s importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earth’s microbial diversity.

Description: Highlights • The upper headwall region of Sahara Slide is mapped for the first time. • The upper headwall region comprises multiple slope failures. • Slope failure occurred on pronounced glide planes at different stratigraphic levels. • Failure is young (~ 2 ka) contradicting the hypotheses of a relatively stable continental margin at present. • This young age requires a reassessment of slope instability and associated risks off NW Africa. Abstract The Sahara Slide Complex in Northwest Africa is a giant submarine landslide with an estimated run-out length of ~ 900 km. We present newly acquired high-resolution multibeam bathymetry, sidescan sonar, and sub-bottom profiler data to investigate the seafloor morphology, sediment dynamics and the timing of formation of the upper headwall area of the Sahara Slide Complex. The data reveal a ~ 35 km-wide upper headwall opening towards the northwest with multiple slide scarps, glide planes, plateaus, lobes, slide blocks and slide debris. The slide scarps in the study area are formed by retrogressive failure events, which resulted in two types of mass movements, translational sliding and spreading. Three different glide planes (GP I, II, and III) can be distinguished approximately 100 m, 50 m and 20 m below the seafloor. These glide planes are widespread and suggest failure along pronounced, continuous weak layers. Our new data suggest an age of only about 2 ka for the failure of the upper headwall area, a date much younger than derived for the landslide deposits on the lower reaches of the Sahara Slide Complex, which are dated at 50–60 ka. The young age of the failure contradicts the postulate of a stable slope off Northwest Africa during times of relative stable sea-level highstands. Such an observation suggests that submarine-landslide risk along the continental margin of Northwest Africa should be reassessed based on a robust dating of proximal and distal slope failures.

Description: We present a conceptual model of fluid circulation in a ridge flank hydrothermal system, the Mariana Mounds. The model is based on chemical data from pore waters extracted from piston cores and from push cores collected by deep-sea research vessel Alvin in small, meter-sized mounds situated on a local topographic high. These mounds are located within a region of heat flow exceeding that calculated from a conductive model and are zones of strong pore water upflow. We have interpreted the chemical data with time-dependent transport-reaction models to estimate pore water velocities. In the mounds themselves pore water velocities reach several meters per year to kilometers per year. Within about 100 m from these zones of focused upflow velocities decrease to several centimeters per year up to tens of centimeters per year. A larger area of low heat flow surrounds these heat flow and topographic highs, with upwelling pore water velocities less than 2 cm/yr. In some nearby cores, downwelling of bottom seawater is evident but at speeds less than 2 cm/yr. Downwelling through the sediments appears to be a minor source of seawater recharge to the basaltic basement. We conclude that the principal source of seawater recharge to basement is where basement outcrops exist, most likely a scarp about 2–4 km to the east and southeast of the study area.

Description: Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To date, 16S rRNA gene sequencing studies have largely utilised different extraction and amplification methodologies to target the microbial communities of a limited number of sponge species, severely limiting comparative analyses of sponge microbial diversity and structure. Here, we provide an extensive and standardised dataset that will facilitate sponge microbiome comparisons across large spatial, temporal and environmental scales. Samples from marine sponges (n = 3569 specimens), seawater (n = 370), marine sediments (n = 65) and other environments (n = 29) were collected from different locations across the globe. This dataset incorporates at least 269 different sponge species, including several yet unidentified taxa. The V4 region of the 16S rRNA gene was amplified and sequenced from extracted DNA using standardised procedures. Raw sequences (total of 1.1 billion sequences) were processed and clustered with a) a standard protocol using QIIME closed-reference picking resulting in 39,543 Operational Taxonomic Units (OTU) at 97% sequence identity, b) a de novo protocol using Mothur resulting in 518,246 OTUs, and c) a new high-resolution Deblur protocol resulting in 83,908 unique bacterial sequences. Abundance tables, representative sequences, taxonomic classifications and metadata are provided. This dataset represents a comprehensive resource of sponge-associated microbial communities based on 16S rRNA gene sequences that can be used to address overarching hypotheses regarding host-associated prokaryotes, including host-specificity, convergent evolution, environmental drivers of microbiome structure and the sponge-associated rare biosphere.

Description: We use numerical climate simulations, paleoclimate data, and modern observations to study the effect of growing ice melt from Antarctica and Greenland. Meltwater tends to stabilize the ocean column, inducing amplifying feedbacks that increase subsurface ocean warming and ice shelf melting. Cold meltwater and induced dynamical effects cause ocean surface cooling in the Southern Ocean and North Atlantic, thus increasing Earth's energy imbalance and heat flux into most of the global ocean's surface. Southern Ocean surface cooling, while lower latitudes are warming, increases precipitation on the Southern Ocean, increasing ocean stratification, slowing deepwater formation, and increasing ice sheet mass loss. These feedbacks make ice sheets in contact with the ocean vulnerable to accelerating disintegration. We hypothesize that ice mass loss from the most vulnerable ice, sufficient to raise sea level several meters, is better approximated as exponential than by a more linear response. Doubling times of 10, 20 or 40 years yield multi-meter sea level rise in about 50, 100 or 200 years. Recent ice melt doubling times are near the lower end of the 10–40-year range, but the record is too short to confirm the nature of the response. The feedbacks, including subsurface ocean warming, help explain paleoclimate data and point to a dominant Southern Ocean role in controlling atmospheric CO2, which in turn exercised tight control on global temperature and sea level. The millennial (500–2000-year) timescale of deep-ocean ventilation affects the timescale for natural CO2 change and thus the timescale for paleo-global climate, ice sheet, and sea level changes, but this paleo-millennial timescale should not be misinterpreted as the timescale for ice sheet response to a rapid, large, human-made climate forcing. These climate feedbacks aid interpretation of events late in the prior interglacial, when sea level rose to +6–9 m with evidence of extreme storms while Earth was less than 1 °C warmer than today. Ice melt cooling of the North Atlantic and Southern oceans increases atmospheric temperature gradients, eddy kinetic energy and baroclinicity, thus driving more powerful storms. The modeling, paleoclimate evidence, and ongoing observations together imply that 2 °C global warming above the preindustrial level could be dangerous. Continued high fossil fuel emissions this century are predicted to yield (1) cooling of the Southern Ocean, especially in the Western Hemisphere; (2) slowing of the Southern Ocean overturning circulation, warming of the ice shelves, and growing ice sheet mass loss; (3) slowdown and eventual shutdown of the Atlantic overturning circulation with cooling of the North Atlantic region; (4) increasingly powerful storms; and (5) nonlinearly growing sea level rise, reaching several meters over a timescale of 50–150 years. These predictions, especially the cooling in the Southern Ocean and North Atlantic with markedly reduced warming or even cooling in Europe, differ fundamentally from existing climate change assessments. We discuss observations and modeling studies needed to refute or clarify these assertions.

Description: 1) With ambitious renewable energy targets, pile driving associated with offshore wind farm construction will become widespread in the marine environment. Many proposed wind farms overlap with the distribution of seals, and sound from pile driving has the potential to cause auditory damage. 2) We report on a behavioural study during the construction of a wind farm using data from GPS/GSM tags on 24 harbour seals Phoca vitulina L. Pile driving data and acoustic propagation models, together with seal movement and dive data, allowed the prediction of auditory damage in each seal. 3) Growth and recovery functions for auditory damage were combined to predict temporary auditory threshold shifts in each seal. Further, M-weighted cumulative sound exposure levels [cSELs(Mpw)] were calculated and compared to permanent auditory threshold shift exposure criteria for pinnipeds in water exposed to pulsed sounds. 4) The closest distance of each seal to pile driving varied from 4·7 to 40·5 km, and predicted maximum cSELs(Mpw) ranged from 170·7 to 195·3 dB re 1μPa2-s for individual seals. Comparison to exposure criteria suggests that half of the seals exceeded estimated permanent auditory damage thresholds. 5) Prediction of auditory damage in marine mammals is a rapidly evolving field and has a number of key uncertainties associated with it. These include how sound propagates in shallow water environments and the effects of pulsed sounds on seal hearing; as such, our predictions should be viewed in this context. 6) Policy implications. We predicted that half of the tagged seals received sound levels from pile driving that exceeded auditory damage thresholds for pinnipeds. These results have implications for offshore industry and will be important for policymakers developing guidance for pile driving. Developing engineering solutions to reduce sound levels at source or methods to deter animals from damage risk zones, or changing temporal patterns of piling could potentially reduce auditory damage risk. Future work should focus on validating these predictions by collecting auditory threshold information pre- and post-exposure to pile driving. Ultimately, information on population-level impacts of exposure to pile driving is required to ensure that offshore industry is developed in an environmentally sustainable manner.

Description: Microwave Limb Sounder and Sounding of the Atmosphere with Broadband Emission Radiometry data provide the first opportunity to characterize the four-dimensional stratopause evolution throughout the life-cycle of a major stratospheric sudden warming (SSW). The polar stratopause, usually higher than that at midlatitudes, dropped by ∼30 km and warmed during development of a major “wave 1” SSW in January 2006, with accompanying mesospheric cooling. When the polar vortex broke down, the stratopause cooled and became ill-defined, with a nearly isothermal stratosphere. After the polar vortex started to recover in the upper stratosphere/lower mesosphere (USLM), a cool stratopause reformed above 75 km, then dropped and warmed; both the mesosphere above and the stratosphere below cooled at this time. The polar stratopause remained separated from that at midlatitudes across the core of the polar night jet. In the early stages of the SSW, the strongly tilted (westward with increasing altitude) polar vortex extended into the mesosphere, and enclosed a secondary temperature maximum extending westward and slightly equatorward from the highest altitude part of the polar stratopause over the cool stratopause near the vortex edge. The temperature evolution in the USLM resulted in strongly enhanced radiative cooling in the mesosphere during the recovery from the SSW, but significantly reduced radiative cooling in the upper stratosphere. Assimilated meteorological analyses from the European Centre for Medium-Range weather Forecasts (ECMWF) and Goddard Earth Observing System Version 5.0.1 (GEOS-5), which are not constrained by data at polar stratopause altitudes and have model tops near 80 km, could not capture the secondary temperature maximum or the high stratopause after the SSW; they also misrepresent polar temperature structure during and after the stratopause breakdown, leading to large biases in their radiative heating rates. ECMWF analyses represent the stratospheric temperature structure more accurately, suggesting a better representation of vertical motion; GEOS-5 analyses more faithfully describe stratopause level wind and wave amplitudes. The high-quality satellite temperature data used here provide the first daily, global, multiannual data sets suitable for assessing and, eventually, improving representation of the USLM in models and assimilation systems.

Description: The Sahara Slide is a giant submarine landslide on the northwest African continental margin. The landslide is located on the open continental slope offshore arid Western Sahara, with a headwall at a water depth of ∼2000 m. High primary productivity in surface waters drives accumulation of thick fine-grained pelagic/hemipelagic sediment sequences in the slide source area. Rare but large-scale slope failures, such as the Sahara Slide that remobilized approximately 600 km3 of sediment, are characteristic of this sedimentological setting. Seismic profiles collected from the slide scar reveal a stepped profile with two 100 m high headwalls, suggesting that the slide occurred retrogressively as a slab-type failure. Sediment cores recovered from the slide deposit provide new insights into the process by which the slide eroded and entrained a volcaniclastic sand layer. When this layer was entrained at the base of the slide it became fluidized and resulted in low apparent friction, facilitating the exceptionally long runout of ∼900 km. The slide location appears to be controlled by the buried headwall of an older slope failure, and we suggest that the cause of the slide relates to differential sedimentation rates and compaction across these scarps, leading to local increases of pore pressure. Sediment cores yield a date of 50–60 ka for the main slide event, a period of global sea level rise which may have contributed to pore pressure buildup. The link with sea level rising is consistent with other submarine landslides on this margin, drawing attention to this potential hazard during global warming.