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  • 1
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    Biomaterials for tissue engineering: summary (1997)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: This article summarizes presentations and discussion at the workshop "Enabling Biomaterial Technology for Tissue Engineering," which was held during the Fifth World Biomaterials Congress in May 1996. Presentations covered the areas of material substrate architecture, barrier effects, and cellular response, including analysis of biomaterials challenges involved in producing specific tissue-engineered products.
    Keywords: Life Sciences (General)
    Type: Tissue engineering (ISSN 1076-3279); Volume 3; 1; 71-3; discussion 73-6
    Format: text
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  • 2
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    Problem areas in the use of the firefly luciferase assay for bacterial detection (1975)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: By purifying the firefly luciferase extract and adding all necessary chemicals but ATP in excess, an assay for ATP was performed by measuring the amount of light produced when a sample containing soluble ATP is added to the luciferase reaction mixture. Instrumentation, applications, and basic characteristics of the luciferase assay are presented. Effect of the growth medium and length of time grown in this medium on ATP per viable E. coli values is shown in graphic form, along with an ATP concentration curve showing relative light units versus ATP injected. Reagent functions and concentration methods are explored. Efforts to develop a fast automatable system to detect the presence of bacteria in biological fluids, especially urine, resulted in the optimization of procedures for use with different types of samples.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: Anal. Appl. of Bioluminescence and Chemiluminescence; p 1-26
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  • 3
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    A comparison of certain extracting agents for extraction of adenosine triphosphate (ATP) from microorganisms for use in the firefly luciferase ATP assay (1975)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: Firefly luciferase ATP assay is used in clinical and industrial applications, such as determination of urinary infection levels, microbial susceptibility testing, and monitoring of yeast levels in beverages. Three categories of extractants were investigated for their extracting efficiency. They were ionizing organic solvents, nonionizing organic solvents, and inorganic acids. Dimethylsulfoxide and formamide represented the ionizing organic solvents, while n-butanol, chloroform, ethanol, acetone, and methylene chloride were used for the nonionizing organic solvents. Nitric acid and perchloric acid were chosen for the inorganic acids category. Pathogens were tested with each solvent. They included: Saccharomyces carlsbergensis, E. coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterobacter species, Proteus mirabilis, Proteus vulgaris, Staphylococcus epidermidis, Streptococcus faecalis, Pseudomonas aeruginosa, and Candida albicans. These results are shown in graphic representations.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: Anal. Appl. of Bioluminescence and Chemiluminescence; p 27-34
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  • 4
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    Automated detection of bacteria in urine (1972)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: A method for detecting the presence of bacteria in urine was developed which utilizes the bioluminescent reaction of adenosine triphosphate with luciferin and luciferase derived from the tails of fireflies. The method was derived from work on extraterrestrial life detection. A device was developed which completely automates the assay process.
    Keywords: BIOTECHNOLOGY
    Type: Significant Accomplishments in Technol., GSFC, 1970; p 188-192
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  • 5
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    The effect of growth phase and medium on the use of the firefly adenosine triphosphate (ATP) assay for the quantitation of bacteria (1975)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: Luciferase assay for adenosine triphosphate (ATP) was used as a rapid method to determine the number of bacteria in a urine sample after nonbacterial components were removed. Accurate cellular ATP values, determined when bacteria were grown in an environment similar to that in which they were found, were necessary for the calculation of bacterial titer in urine. Cellular ATP values vary depending on the extraction method, the cell growth phase, and cell growth conditions. ATP per cell values of stationary E. coli grown in urine were two times greater than ATP per cell values of cells grown in trypticase soy broth. Glucose and urea were examined as possible components responsible for the cellular ATP variation.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: Anal. Appl. of Bioluminescence and Chemiluminescence; p 35-41
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  • 6
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    A rapid method for the determination of microbial susceptibility using the firefly luciferase assay for adenosine triphosphate (ATP) (1975)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: Luciferase assay for adenosine triphosphate (ATP) was optimized for pure bacteria in broth in order to evaluate if changes in bacterial ATP content could be used as a rapid measure of antibiotic effect on microorganisms. Broth cultures of log phase bacteria were incubated at 310 K (37 C) for 2.5 hours at antimicrobial concentrations which resulted in the best discrimination between sensitive and resistant strains. Eighty-seven strains of 11 bacterial species were studied for their susceptibility to 12 commonly used antimicrobial agents: ampicillin, Penicillin G, nafcillin, carbenicillin, cephalothin, tetracycline, erythromycin, clindamycin, gentamicin, nitrofurantoin, colistin, and chloramplenicol. The major advantage of the ATP system over existing methods of rapid microbial susceptibility testing is that the assay can be made specific for bacterial ATP.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: Anal. Appl. of Bioluminescence and Chemiluminescence; p 43-44
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  • 7
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    Rapid, quantitative determination of bacteria in water (1978)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: A bioluminescent assay for ATP in water borne bacteria is made by adding nitric acid to a water sample with concentrated bacteria to rupture the bacterial cells. The sample is diluted with sterile, deionized water, then mixed with a luciferase-luciferin mixture and the resulting light output of the bioluminescent reaction is measured and correlated with bacteria present. A standard and a blank also are presented so that the light output can be correlated to bacteria in the sample and system noise can be substracted from the readings. A chemiluminescent assay for iron porphyrins in water borne bacteria is made by adding luminol reagent to a water sample with concentrated bacteria and measuring the resulting light output of the chemiluminescent reaction.
    Keywords: LIFE SCIENCES (GENERAL)
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  • 8
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    Method of detecting and counting bacteria in body fluids (1973)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: A novel method is reported for determining bacterial levels in urine samples, which method depends on the quantitative determination of bacterial adenosine triphosphate (ATP) in the presence of non-bacterial ATP. After the removal of non-bacterial ATP, the bacterial ATP is released by cell rupture and is measured by an enzymatic bioluminescent assay using an enzyme obtained from the firefly.
    Keywords: BIOSCIENCES
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  • 9
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    Laboratory procedures manual for the firefly luciferase assay for adenosine triphosphate (ATP) (1975)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: A manual on the procedures and instruments developed for the adenosine triphosphate (ATP) luciferase assay is presented. Data cover, laboratory maintenance, maintenance of bacterial cultures, bacteria measurement, reagents, luciferase procedures, and determination of microbal susceptibility to antibiotics.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: NASA-TM-X-70926 , X-726-75-1
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  • 10
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    Automatic device for shell freezing of liquids (1973)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: Unit is insulated enclosure designed to contain liquid nitrogen. It also includes set of stainless steel rotating rods for holding vessels containing liquids to be frozen, and electric drive mechanism for rotating these rods. Present device will accept 10 vessels at a time.
    Keywords: MATERIALS
    Type: GSC-11737
    Format: application/pdf
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