ISSN:
1573-0689
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Mathematical analysis of the equation for the radioimmunoassay, R2+R(1+Kp+Kp*−Kq)−Kq=0, gives a general solution for the dose of maximum precision, ∂2R/∂p2, of the assay. This solution, (R−c1+Kp)(R−c2), and the radioimmunoassay equation can be used to determine theoretical conditions for maximum precision of a radioimmunoassay at any dose p. A theoretically optimal antibody concentration, q, can be calculated for precision at a critical concentration of interest, p. This is dependent on the affinity constant, K, of the particular antibody being used and, potentially, the amount of labeled tracer, p*. This solution idealizes the radioimmunoassay to a one affinity constant system. Though this may be achievable, particularly with monoclonal antibodies or receptors, the applicability needs to be explored experimentally.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01867801
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