ISSN:
1430-4171
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The quest for a substantial increase in the peak capacity and, therefore, in the number of compounds that can be separated in a single chromatographic run, led to the development of multidimensional techniques in high-performance liquid chromatography (HPLC). The “classical” method of collecting fractions from the initial separation and separating them further in the secondary steps is very powerful but its fully automated “online” version requires quite complex instrumentation. In contrast, development of new chromatographic media with segregated biomodal chemistries allows the 2-D separations to be achieved in a single column with standard HPLC equipment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s00897970151a
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