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Determination of Scoparone in Citrus Roots by Micellar Electrokinetic Capillary Chromatography (2000)

Aucamp, Jean P. ; Kotze, Sophia S. ; Fourie, Andries ; [et al.]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 23 (2000), S. 519-521

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ISSN: 0935-6304

Keywords: Scoparone ; phytoalexin ; citrus ; MEKC ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: ---A new MEKC method has been developed to determine the amount of the phytoalexin, scoparone, in Citrus roots. The separation and analysis was achieved with a running buffer of 100 mM SDS, 25 mM phosphate and 12% (v/v) methanol pH 6.2. Separation was performed at 12 kV with 25°C and UV detection at 200 nm. A near complete recovery of scoparone was obtained with the extraction procedure. The MEKC method was compared with a fluorescence TLC method. The detection limit for scoparone with the MEKC method (2 μg/mL) was better than the TLC method (10 μg/mL).

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

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