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  • 1
    Publication Date: 2021-05-19
    Description: In this study, the antibacterial effects of essential oil extracted from 4 medicinal plants, (Thymus deansis, Satureja bachtiarica, Cuminus cyminam and Myrtus communis) carvacrol, tymol, florfenicol and tetracycline against three pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri and Lactococcus garvieae) were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) of the essential oils were evaluated after preparation of serial dilution. Inhibition zone were also determined on Muller Hinton agar medium. According to the results, the largest inhibition zone was observed from S. bachtiarica and C. cyminam essential oils against A. hydrophila, Y. ruckeri and L. garvieae. The obtained results showed different MICs and MBCs for different bacteria; however, in most cases the essential oils were more effective than tetracycline. Tymol, florfenicol and S. bachtiarica essential oil demonstrated the highest inhibitory and bactericidal effects against A. hydrophila, Y. ruckeri and L. garvieae, respectively. The MIC and MBC of tymol against A. hydrophila were 6.3 and 12.5 µg ml-1 , respectively. The MIC and MBC of florfenicol against L. garvieae were 3.125 and 6.25 and of S. bachtiarica essential oil against Y. ruckeri were 6.25 and 12.5 µg ml-1 , respectively. It can be concluded that the sensitivity of the bacteria studied to the essential oils are different. However, considering the antimicrobial effects of some essential oils, it is necessary to evaluate their beneficial effects in prevention and treatment of bacterial diseases in fish.
    Description: Published
    Keywords: Antibacterial ; Antibacterial effects ; Aeromonas hydrophila ; Yersinia ruckeri ; Lactococcus garvieae ; Cuminus cyminam ; Myrtus communis ; Plant ; Medicinal
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.55-64
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  • 2
    Publication Date: 2021-05-19
    Description: Streptococcosis has been defined as a hemorrhagic septicemia in many fish species especially rainbow trout. This disease causes serious economic losses due to high mortality in fish and the bacteria responsible is also considered as a zoonotic pathogen. The aim of this study was identification of different causative agents of warm water streptococcosis and evaluation of the capsule gene in Lactococcus garvieae isolates collected from rainbow trout in Chaharmahal - Bakhtiari Province, Iran. A total of 430 clinically suspected fish were collected from 43 rainbow trout farms. The samples were analyzed using biochemical methods and multiplex-PCR. The results indicated that samples of 36 fish farms (83.7%) were infected with one of the studied bacteria. The infection by L. garvieae was found to be more than that by other species (30 of 36 farms, 83%). Infection by S. iniae and S. uberis was found in nine and one fish farm, respectively. Presence of virulence genes was studied in L. garvieae isolates by PCR assay. According to the results 34 of 50 isolates (68%) contained the capsule gene. The results revealed high prevalence of virulent L. garvieae in rainbow trout farms which should be considered in preventional policies such as vaccine production and also in public health.
    Description: Published
    Keywords: Fish disease ; Lactococcus garvieae ; Streptococcosis ; Virulence gene ; Rainbow trout ; PCR ; Molecular ; Identification
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.836-845
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  • 3
    Publication Date: 2021-05-19
    Description: This study was carried out to investigate occurrence of parasite in selected cyprinid fish species in Gandoman Lagoon, in the vicinity of Gandoman City, Chaharmahal & Bakhtyari Province. A total of 90 fish specimens including Cyprinus carpio, Carassius auratus gibelio, Capoeta aculeata , C.damascina, Chondrostoma regium and Alburnus alburnus from Gandoman were examined during spring and summer 2007. Ten species of parasites were found, including Ichthyophthirius multifiliis, Trichodina sp. (Cilliophora), Myxobolus musayevi, Myxobolus sp. (Myxozoa), Dactylogyrus extensus, D.lenkorani (Monogenea), Diplostomum spathaceum, Tylodelphys clavata (Digenea). Argulus foliaceus and Lernea cyprinacea (Crustaceans). 77.7% of fishes were infected to parasites and 22.3 % did not show any infection. Chondrostoma regium and Alburnus alburnus are reported as new hosts for D. extensus.
    Description: Published
    Keywords: Alburnus alburnus ; Chondrostoma regium ; Lernea cyprinacea ; Argulus foliaceus ; Tylodelphys clavata ; Diplostomum spathaceum ; Dactylogyrus extensus ; Myxobolus musayevi ; Ichthyophthirius multifiliis ; Fish parasites
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.464-471
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  • 4
    Publication Date: 2021-05-19
    Description: The aim of this research was to study the occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp. in crayfish from Azerbaijan Province using culture method and PCR assay. A total of 55 isolates were collected from 97 studied samples. Vibrio spp., A. hydrophila, E. coli and Campylobacter spp. were detected in 26 samples (26.8%), 12 samples (12.3%), 15 samples (15.46%) and 2 samples (2.06%), respectively. Among Vibrio isolates, Vibrio vulnificus (11.3%) was the species most frequently detected followed by V. harveyi (7.2%), V. alginolyticus (2.06%) and V. mimicus (1.03%). The results of this study indicated that crayfish from the studied area contain pathogens relevant to public health.
    Description: Published
    Keywords: Fish disease ; Astacus leptodactylus ; Aeromonas hydrophila ; Escherichia coli ; Vibrio spp. ; Crayfish ; Vibrio ; Aeromonas ; E. coli ; Campylobacter
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.944-954
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  • 5
    Publication Date: 2021-05-19
    Description: Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms Intraperitoneal injection (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×107 cfu as LD50 96 hrs of Lactococcus garvieae were IP injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing concentrations of the N. sativa essential oil concentration, with a significant difference with control groups (p〈0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate although S. bachtiarica was not effective on immune parameters of the studied fish.
    Description: Published
    Keywords: Oncorhynchus mykiss ; Rainbow trout ; Lactococcus garvieae ; Nigella sativa ; Satureja bachtiarica ; Streptococcosis ; Lactococcosis ; Adjuvant
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.95-106
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  • 6
    Publication Date: 2021-05-19
    Description: The present study was done to evaluate the presence of antibiotic resistance genes in Lactococcus garvieae isolated from cultured rainbow trout, West Iran.The isolates were examined for antimicrobial resistance using disc diffusion method. Of the 24 strains tested, 21 were resistant to ampicillin (87.5%), 9 to erythromycin (37.5%) and 19 to tetracycline (79.1%). Fourteen strains were resistant to four antibiotics, 8 resistant to five antibiotics and 2 to six antibiotics.The strains were also characterized for their genotypic resistance profiles. The results revealed that all 24 isolates contained one to three of the antibiotic resistance genes. StrA, tetS and ermB genes coding for streptomycin, tetracycline and erythromycin resistance were found in 7, 10 and 9 isolates, respectively and sulfamethoxazole resistance gene, sul2, was not detected in the examined isolates. High levels of antibiotic resistance and detection of resistance genes in L. garvieae strains should be considered as a potential danger for trout culture as well as for public health.
    Description: Published
    Keywords: Fish disease ; Lactococcus garvieae ; Antimicrobial resistance genes ; Rainbow trout ; Detection ; Antibiotics ; Genes
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.221-229
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  • 7
    Publication Date: 2021-05-19
    Description: The present study was aimed to evaluate the antimicrobial resistance and the presence of antibiotic resistance genes in Vibrios spp. isolated from seafood. A total of 72 isolates of Vibrio in 6 species including V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. harveyi, V. mimicus and V. cholerae were examined. The results revealed that all isolates were expressing multiple antibiotic resistances. Of the 72 strains tested, 70 were resistant to ampicillin (97.2%), 60 to gentamycin (83.3%) and 56 to penicillin (77.7%). Eight strains were resistant to 4 antibiotic, 19 resistant to five antibiotics, 10 to six antibiotics, 34 to seven antibiotics and one to eight antibiotics. Results also revealed that 20 Vibrio strains (27.7% of total examined strains) contained one to three of the antibiotic resistance genes. StrB, tetS and ermB genes coding for streptomycin, tetracycline and erythromycin resistance were found in 18, 6, 5 isolates, respectively and Sulfamethoxazole resistance gene, sul2, was not detected in this study. Detection of resistance genes in Vibrio strains obtained from seafood is considered as a potential danger for consumers and also suggests that these resistance determinants might be further disseminated in habitats, thus constituting a serious health risks to human.
    Description: Published
    Keywords: Fish processing ; Vibrio spp. ; Antimicrobial resistance genes ; Seafood
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.618-626
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  • 8
    Publication Date: 2021-05-19
    Description: The current study was done with the aim of identification of ectoparasites of some aquarium fish including Carassius auratus auratus, Poecilia reticulata, Pterophyllum scalar and Colisa lalia. A total of 140 fish were randomly collected from Isfahan city in 2013, and were transported alive in appropriate condition to Fishery Reserch Center, Islamic Azad University, Shahrekord. Fish were kept in separate aquariums and were individually studied. The obtained results revealed that the studied fish were infested by Ichthyophthirius multifiliis, Trichodina sp., Dactylogyrus anchoratus, Dactylogyrus spiralis, Dactylogyrus sp1, Dactylogyrus sp2, Trianchoratus sp., Ancyrocephalus pseudorasbora and Gyrodactyrus sp. Overall, 64 0f 140 studied fish (46%) were found to be infested by parasites and the remaining were not infested. The highest infestation rate was found in gold fish (75%) and the lowest was observed in guppy (2.5%). A. pseudorasborae and Trianchoratus sp. are reported for the first time in Iran which were collected from gold fish and dwarf gourami, respectively. Gold fish is also reported as new host for D. spiralis.
    Description: Published
    Keywords: Dwarf gourami ; Gold fish ; Angel fish ; Guppy ; Parasite ; Carassius auratus auratus ; Poecilia reticulata ; Pterophyllum scalar ; Colisa lalia ; Ichthyophthirius multifiliis ; Trichodina sp. ; Dactylogyrus spiralis ; Trianchoratus sp. ; Ancyrocephalus pseudorasbora ; Gyrodactyrus sp. ; pseudorasborae ; Trianchoratus sp. ; Fish ; Identification
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.87-94
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  • 9
    Publication Date: 2021-05-19
    Description: The main objectives of this study were to study the parasitic infestation of Lernaea cyprinacea in 4 cyprinids from the Choghakhor Lagoon, Chaharmahal - Bakhtyari Province, west of Iran. A total of 180 cyprinids including Cyprinus carpio (n=101), Carassius auratus (n=47), Capoeta aculeata (n=10) and Alburnus alburnus (n=22) caught, and were studied for Lernaea cyprinacea infestation. Prevalence (C. carpio 61.4, C. auratus 87.2, C. aculeata 70 and A. alburnus 68.2), intensity of infection (C. carpio range 1 to 5, mean 2.1 C. auratus range 1 to 6, mean 1.9 C. aculeata range 1 to 5, mean 2.4 A. alburnus range 1 to 2, mean 1.1), and abundance (C. carpio 1.3, C. auratus 1.6, C. aculeata 1.7 and A. alburnus 0.8) varied with the fish species. A statistically significant difference was found between infestation by L. cyprinacea and fish species (p=0.01), although no statistically significant difference was found between infestation and weight, length and age of the studied fishes (p〉0.05). The prevalence was also significantly different (p=0.0) in studying seasons. Population dynamics of L. cyprinacea on fish hosts was studied. The results show that the preferred site of the parasite was body lateral surfaces followed by caudal , dorsal , pectoral and anal fins (P=0.0).
    Description: Published
    Keywords: Fish disease ; Copepoda ; Carassius auratus ; Alburnus alburnus ; Capoeta aculeata ; Lernaea cyprinacea ; Fish parasite ; Investigation ; Species ; Fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.680-688
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  • 10
    Publication Date: 2021-05-19
    Description: Zagros tooth-carp (Aphanius vladykovi) which belongs to cyprinodontidae family is one of the most important native fish species of Iran. In this study which was conducted in summer 2015, a total of 50 individuals of A. vladykovi were caught from Shalamzar and Salm Lakes in Chaharmahal va Bakhtiari Province and were examined for parasites. The results of this study indicated that all the examined fish were infested by parasites. Different parasite species including Ichthyophthirius multifiliis, Ichthyobodo necator, Gyrodactylus sp., Allocreadium isoporum and metacercaria of Ornithodiplostomum sp. were found in the examined fish. Among them, the highest infestation rate belonged to Ornithodiplostomum sp. as 68 percent of the studied fish were infested by this parasite. The infestation rate of Ornithodiplostomum sp.in fishes from Shalamzar and Salm Lakes were 41.66 and 92.3 percent, respectively. The results of this study indicated that Zagros tooth-carp fish is highly infested with different parasite species affecting population of this fish.
    Description: Published
    Keywords: Ornithodiplostomum sp. ; Aphanius vladykovi ; Zagros tooth-carp ; Ichthyophthirius multifiliis ; Ichthyobodo necator ; Gyrodactylus sp. ; Allocreadium isoporum ; Fish parasites ; Species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.159-163
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