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  • 1
    Electronic Resource
    Electronic Resource
    An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 345-353 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies (i.e. “mono-”, “bi-” and “tri-cistronic” vectors) to achieve stable secretion from BHK cells of a glycosylated antibody-cytokine fusion protein designed for use in anti-tumour therapy. It describes selection of a robust and effective production cell line based on stability of secretion of the product, quality of mRNA and protein products and performance in in vitro bioassays for potency. The data obtained at this stage were utilised in the selection of a suitable candidate production cell line. The relative productivity and general performance of the cells in stirred tank and fixed bed culture systems indicated that a variety of cell culture technologies provided robust tools for production of a highly selected cell clone. Consistency of the product glycosylation was determined by analysis of released oligosaccharides using matrix-assisted laser desorption ionisation – time of flight mass spectrometry and high-performance anion exchange chromatography. These investigations showed consistent expression of three glycoforms of the fusion protein which varied in their relative proportions in different culture systems and at different time points in a fixed bed reactor with continuous perfusion. In conclusion, this study dealt with a range of important scientific and technical issues which are essential for regulatory approval and commercial success of a recombinant protein and elucidates some useful markers for process development for similar recombinant biologicals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051530
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  • 2
    Electronic Resource
    Electronic Resource
    Metabolic responses to different glucose and glutamine levels in baby hamster kidney cell culture (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 579-585 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this work, a BHK21 clone producing a recombinant antibody/cytokine fusion protein was used to study the dependence of cell metabolism on the glucose and glutamine levels in the culture medium. Results obtained indicate that both glucose and glutamine consumptions show a Michaelis-Menten dependence on glucose and glutamine concentrations respectively. A similar dependence is also observed for lactate and ammonia productions. The estimated value of the Michaelis constant for the dependence of lactate production on glucose (K Glc Lac) was 1.4 ± 0.1 mM and for the dependence of ammonia production on glutamine (K Gln Amm) was 0.25 ± 0.11 mM and 0.10 ± 0.03 mM, at glucose concentrations of 0.28 mM and 5.6 mM respectively. At very low glucose concentrations, the glucose to lactate yield decreased markedly, showing a metabolic shift towards lower lactate production. This␣metabolic shift was also confirmed by the significant increase in the specific oxygen consumption rate also observed at low glucose concentrations. Although it was␣highly dependent on glucose concentration, the oxygen consumption also increased with the increase in␣glutamine concentration. At very low glutamine concentrations, the glutamine to ammonia yield increased, showing a more efficient glutamine metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051435
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  • 3
    Electronic Resource
    Electronic Resource
    Electrical resistivity and local magnetic order in random anisotropy amorphous ferromagnets : 38th Annual Conference on Magnetism and Magnetic Materials (1994)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 75 (1994), S. 6513-6515 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In ferromagnetic systems with random anisotropy (RA), long-range magnetic order is destroyed and the correlation function of the magnetization exhibits an exponential form e−r/Rf, where the correlation length Rf corresponds to the size of Imry–Ma domains. Also, the law of approach to magnetic saturation follows a 1/(square root of)H law [ferromagnet with wandering axis (FWA) regime]. We have calculated the effect of magnetic correlations on the electrical resistivity and magnetoresistance of a RA ferromagnetic system at low temperatures. We find that in zero magnetic field RA introduces a positive contribution into the electrical resistivity, which increases with the anisotropy to exchange ratio, D/T. In the FWA regime the magnetoresistance has a logarithmic dependence on the magnetic field with a slope Δρ/(ρ ln H) inversely proportional to Rf. These features have been observed in an experimental study of the magnetoresistance at low temperatures (T≈4 K) of the amorphous series (DyxGd1−x)Ni in which the D/T ratio can be varied from near zero up to one from GdNi to DyNi. A quantitative analysis of the results enables the direct determination of the correlation length Rf and of the volume of structural correlations Ωc. We find that Rf varies from about 12 A(ring) in DyNi up to 300 A(ring) in (Dy0.1Gd0.9)Ni and Ωc is the same throughout the series (Ωc≈500 A(ring)3) in excellent agreement with the values determined from magnetic measurements on the approach to saturation. These results provide an independent confirmation of the exponential decay with distance and field dependence of magnetic correlations in random anisotropy systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.356932
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  • 4
    Electronic Resource
    Electronic Resource
    Transport properties in amorphous Ux-T1−x films (T=Fe, Ni, Gd, Tb, and Yb) (invited) (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 5453-5458 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We describe the competing magnetic, localization, and phonon effects on the transport properties of amorphous magnetic UxT1−x films, with T=Fe, Ni, Gd, Tb, and Yb. Amorphous UxFe1−x films change from collinear to random ferromagnetism as x increases, and the temperature dependence of the resistivity denotes the competing effects of spin-flip and non-spin-flip exchange scattering processes. The resistivity has a minimum at Tf rising sharply below this temperature. The sign of the magnetic resistivity and the magnetoresistance indicates 〈Si ⋅Sj 〉〉0, while the anisotropic magnetoresistance indicates a local exchange gap. Amorphous UxGd1−x and a-UxTb1−x are, respectively, spin glasses and random anisotropy dominated systems. The resistivity increases smoothly through Tf and has a slight upturn at low temperatures that we associate with weak localization. The magnetoresistance is negative in both systems and the anisotropic magnetoresistance is null, although the applied field induces anisotropic behavior in the Tb containing films (asperomagnets). All samples show quadratic and positive field dependence of magnetoresistance well inside the paramagnetic regime, and a linear regime below Tf. At low temperatures and in the a-UxGd1−x films, negative (H)1/2 and H2 regimes occur and are associated with weak localization processes dominated by the inelastic mean free path.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.342346
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  • 5
    Electronic Resource
    Electronic Resource
    Modifications of weak localization contributions to magnetoresistance due to magnetic impurities in amorphous DyxY1−xNi alloys (invited) : The 5th Joint MMM−Intermag Conference (1991)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 70 (1991), S. 5813-5818 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: High-field magnetoresistance experiments performed on thick amorphous alloys offer a simple way to study three-dimensional (3D) weak localization of conduction electrons. After the precursor work of Fert et al. on nonmagnetic amorphous alloys, we found it interesting to study how these effects would disappear under substitutions of magnetic impurities (1% to 10% of Dy) in a nonmagnetic amorphous alloy (YNi). The experiments, performed between 1.5 to 50 K and in magnetic fields up to 20 T, showed (i) in YNi, characteristic features of the magnetoresistance due to weak localization under strong spin-orbit scattering and (ii) in DyxY1−xNi, a coexistence of weak localization effects with the classical contribution of spin alignment by the applied magnetic field, saturating at negative values. This last contribution dominates the behavior of Dy-richer samples whereas weak localization is clearly observed for x≤3%. In all the samples a dramatic increase of the initial magnetoresistance slope Δρ/ρH2 (where ρ=resistivity and H=applied field) is observed when magnetic impurity concentrations increase. We explain this increase of weak localization effects, in the framework of the available weak localization theory, by an enhancement of the Zeeman spin splitting due to interactions between localized (4f ) and delocalized (d, s) electronic states.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.350120
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  • 6
    Electronic Resource
    Electronic Resource
    Studies of Baby Hamster Kidney Natural Cell Aggregation in Suspended Batch Cultures (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 745 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb44368.x
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of in vitro stability of large unilamellar liposomes coated with a modified polysaccharide (O-palmitoylpullulan) (1996)
    Springer
    Journal of materials science 7 (1996), S. 301-303 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Aiming at encapsulation of a hydrosoluble drug, large unilamellar liposomes (LUV) of egg phosphatidylcholine (PC) were coated with a natural polysaccharide derivative, O-palmitoylpullulan (OPP), and its in vitro stability evaluated using fluorescent probes. This coating (in OPP/PC weight ratio of 3) improved significantly the in vitro stability of LUV by decreasing both the permeability and fluidity of the liposomal membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00058571
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  • 8
    Electronic Resource
    Electronic Resource
    Toxicity Evaluation of a Municipal Dump Leachate Using Zebrafish Acute Tests (2000)
    Springer
    Bulletin of environmental contamination and toxicology 64 (2000), S. 107-113 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001289910017
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  • 9
    Electronic Resource
    Electronic Resource
    Stationary phase corrections in the process of bosonization of multi-quark interactions (2006)
    Springer
    The European physical journal 46 (2006), S. 225-233 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: AbstractThe functional integration over the auxiliary bosonic variables of cubic order related with the effective action of the Nambu–Jona–Lasinio model with ’t Hooft term has recently been obtained in the form of a loop expansion. Even numbers of loops contribute to the action, while odd numbers of loops are assigned to the measure. We consider the two-loop corrections and analyse their effect on the low-lying pseudoscalar and scalar mass spectra, quark condensates and weak decay constants. The results are compared to the leading order calculations and other approaches.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1140/epjc/s2006-02488-7
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  • 10
    Electronic Resource
    Electronic Resource
    Simulation of chaotic behaviour in population dynamics (1998)
    Springer
    The European physical journal 1 (1998), S. 393-396 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 87.10.+e General, theoretical, and mathematical biophysics (including logic of biosystems, quantum biology, and relevant aspects of thermodynamics, information theory, cybernetics, and bionics) - 05.45.+b Theory and models of chaotic systems - 02.70.Lq Monte Carlo and statistical methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: In the time evolution of populations, many attractors can be found: fixed points, limit cycles and chaotic regimes. Usually, chaotic behaviour is observed in species which have well defined breeding seasons and a high fertility rate. Different mathematical models have been used in order to simulate those regimes. In this paper, we use the bitstring model introduced to simulate the evolution of age-structured populations -- the Penna Model -- to simulate a sort of cyclic and chaotic behaviours. In comparison with the standard logistic map, our results show a time changing parameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100510050199
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