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  • 1
    Keywords: Water. ; Hydrology. ; Freshwater ecology. ; Marine ecology. ; Environmental chemistry. ; Environmental management. ; Environmental engineering. ; Biotechnology. ; Bioremediation. ; Water. ; Freshwater and Marine Ecology. ; Environmental Chemistry. ; Environmental Management. ; Environmental Engineering/Biotechnology.
    Description / Table of Contents: Section I Overview -- 1 Introduction to the volume -- 2 Deep-water oil and gas production in the Gulf of Mexico, and related global trends -- 3 Spilled oil composition and the natural carbon cycle: The true drivers of environmental fate and effects of oil spills -- Section II Geological, Chemical, Ecological and Physical Oceanographic Settings and Baselines for Deep Oil Spills in the Gulf of Mexico -- 4 An overview of the geologic origins of hydrocarbons and production trends in the Gulf of Mexico -- 5 Gulf of Mexico (GoM) bottom sediments and depositional processes: A baseline for future oil spills -- 6 Benthic faunal baselines in the Gulf of Mexico: A precursor to evaluate future impacts -- 7 Linking abiotic variables with macrofaunal and meiofaunal abundance and community -- 8 The asphalt ecosystem of the southern Gulf of Mexico: abyssal habitats across space and time -- 9 Geochemical and faunal characterization in the sediments off the Cuban north and northwest coast -- 10 Mapping isotopic and dissolved organic matter baselines in waters and sediments of Gulf of Mexico -- 11 Toward a predictive understanding of the benthic microbial community response to oiling on the northern Gulf of Mexico coast -- 12 Combining isoscapes with tissue-specific isotope records to re-create the geographic histories of fish -- 13 The utility of stable and radio isotopes in fish tissues as biogeochemical tracers of marine oil spill food web effects -- 14 Modernizing protocols for aquatic toxicity testing of oil and dispersant -- 15 Polycyclic aromatic hydrocarbon baselines in Gulf of Mexico fishes -- 16 Case Study: Using a combined laboratory, field, and modeling approach to assess oil spill impacts -- Section III Simulations of Future Deep Spills -- 17 Testing the effect of MOSSFA (Marine Oil Snow Sedimentation and Flocculent Accumulation) events in benthic microcosms -- 18 Physical processes influencing the sedimentation and lateral transport of MOSSFA in the NE Gulf of Mexico -- 19 Simulating deep oil spills beyond the Gulf of Mexico -- Section IV Comparisons of likely impacts from simulated spills -- 20 Comparison of the spatial extent, impacts to shorelines, and ecosystem and 4-dimensional characteristics of simulated oil spills -- 21 A predictive strategy for mapping locations where future MOSSFA events are expected -- 22 Connectivity of Gulf of Mexico continental shelf fish populations and implications of simulated oil spills -- 23 Evaluating the effectiveness of fishery closures for deep oil spills using a 4-dimensional model -- 24 As Gulf oil extraction goes deeper, who is at risk? Community structure, distribution, and connectivity of the deep-pelagic fauna -- 25 Evaluating impacts of deep oil spills on oceanic marine mammals -- 26 Comparative environmental sensitivity of offshore Gulf of Mexico waters potentially impacted by ultra-deep oil well blowouts -- Section V Preparing for and Responding to the Next Deepwater Spill -- 27 Preparing for the inevitable: ecological and indigenous community impacts of oil spill-related mortality in the United States Arctic marine ecosystem -- 28 Summary of contemporary research on use of chemical dispersants for deep sea oil spills -- 29 Perspectives on research, technology, policy and human resources for improved management of ultra-deep oil and gas resources and responses to oil spills -- Index.
    Abstract: It has often been said that generals prepare for the next war by re-fighting the last. The Deepwater Horizon (DWH) oil spill was unlike any previous – an underwater well blowout 1,500 meters deep. Much has been learned in the wake of DWH and these lessons should in turn be applied to both similar oil spill scenarios and those arising from “frontier” explorations by the marine oil industry. The next deep oil well blowout may be at 3,000 meters or even deeper. This volume summarizes regional (Gulf of Mexico) and global megatrends in marine oil exploration and production. Research in a number of key areas including the behavior of oil and gas under extreme pressure, impacts on biological resources of the deep sea, and the fate of oil and gas released in spills is synthesized. A number of deep oil spills are simulated with detailed computer models, and the likely effects of the spills and potential mitigation measures used to combat them are compared. Recommended changes in policies governing marine oil exploration and development are proposed, as well as additional research to close critical and emerging knowledge gaps. This volume synthesizes state-of-the-art research in deep oil spill behavior and response. It is thus relevant for government and industry oil spill responders, policy formulators and implementers, and academics and students desiring an in-depth and balanced overview of key issues and uncertainties surrounding the quest for deep oil and potential impacts on the environment.
    Type of Medium: Online Resource
    Pages: XII, 542 p. 167 illus., 138 illus. in color. , online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030129637
    DDC: 551.48
    Language: English
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  • 2
    Keywords: Water. ; Hydrology. ; Freshwater ecology. ; Marine ecology. ; Environmental chemistry. ; Environmental management. ; Environmental engineering. ; Biotechnology. ; Bioremediation. ; Water. ; Freshwater and Marine Ecology. ; Environmental Chemistry. ; Environmental Management. ; Environmental Engineering/Biotechnology.
    Description / Table of Contents: Section I. Introduction -- 1. Introduction to the Volume -- Section II. Physics and Chemistry of Deep Oil Well Blowouts -- 2. The importance of understanding fundamental physics and chemistry of deep oil blowouts -- 3. Physical and chemical properties of oil and gas under reservoir and deep-sea conditions -- 4. Jet formation at the blowout site -- 5. Behavior of rising droplets and bubbles – impact on the physics of deep-sea blowouts and oil fate -- Section III. Transport and Degradation of Oil and Gas from Deep Spills -- 6. The importance of understanding transport and degradation of oil and gasses from deep sea blowouts -- 7. Biodegradation of petroleum hydrocarbons in the deep sea -- 8 Partitioning of organics between oil and water phases with and without the application of dispersants -- 9. Dynamic coupling of near-field and far-field models -- 10. Effects of oil properties and slick thickness on dispersant field effectiveness and oil fate -- 11. Far-field modeling of a deep-sea blowout: sensitivity studies of initial conditions, biodegradation, sedimentation and sub-surface dispersant injection on surface slicks and oil plume concentrations -- Section IV. Oil Spill Records in Deep Sea Sediments -- 12. Formation and sinking of MOSSFA (Marine Oil Snow Sedimentation and Flocculent Accumulation) events: Past and Present -- 13. The sedimentary record of MOSSFA events in the Gulf of Mexico: A comparison of the Deepwater Horizon (2010) and Ixtoc 1 (1979) oil spills -- 14. Characterization of the sedimentation associated with the Deepwater Horizon blowout: depositional pulse, initial response, and stabilization -- 15. Applications of FTICR-MS in oil spill studies -- 16. Changes in redox conditions of surface sediments following the Deepwater Horizon and Ixtoc 1 events -- 17. Long-term preservation of oil spill events in sediments: the case for the Deepwater Horizon spill in the northern Gulf of Mexico -- 18. Effect of marine snow on microbial oil degradation -- 19. Molecular legacy of the 1979 Ixtoc 1 oil spill in deep-sea sediments of the southern Gulf of Mexico -- 20. 40 years of weathering of coastal oil residues in the southern Gulf of Mexico -- Section V. Impacts of Deep Spills on Plankton, Fishes, and Protected Resources -- 21. Overview of ecological impacts of deep spills -- 22. Deep-sea benthic faunal impacts and community evolution before, during and after the Deepwater Horizon event -- 23. Impact and resilience of benthic foraminifera in the aftermath of the Deepwater Horizon and Ixtoc 1 oil spills -- 24. Chronic sublethal effects observed in wild caught fish following two major oil spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1 -- 25. Impacts of deep spills on fish and fisheries -- 26. Impacts of the Deepwater Horizon oil spill on marine mammals and sea turtles -- Section VI. Toxicology of Deep Oil Spills -- 27. Ecotoxicology of deep ocean spills -- 28 A synthesis of Deepwater Horizon oil, chemical dispersant and chemically dispersed oil aquatic standard laboratory acute and chronic toxicity studies -- 29. Digging deeper than LC/EC50: non-traditional endpoints and non-model species in oil spill toxicology -- 30. Genetics and oil: transcriptomics, epigenetics and population genomics as tools to understand animal responses to exposure across different time scales -- Section VI. I Ecosystem-level modeling of deep oil spill impacts -- 31. A synthesis of top down and bottom up impacts of the Deepwater Horizon oil spill using ecosystem modeling -- 32. Comparing ecosystem model outcomes between Ixtoc 1 and Deepwater Horizon oil spills -- 33. Effects of the Deepwater Horizon oil spill on Human Communities: Catch and Economic Impacts -- Section VIII. Summary -- 34. Summary of Major Themes – Deep Oil Spills -- Index.
    Abstract: The demand for oil and gas has brought exploration and production to unprecedented depths of the world’s oceans. Currently, over 50% of the oil from the Gulf of Mexico now comes from waters in excess of 1,500 meters (one mile) deep, where no oil was produced just 20 years ago. The Deepwater Horizon oil spill blowout did much to change the perception of oil spills as coming just from tanker accidents, train derailments, and pipeline ruptures. In fact, beginning with the Ixtoc 1 spill off Campeche, Mexico in 1979-1980, there have been a series of large spill events originating at the sea bottom and creating a myriad of new environmental and well control challenges. This volume explores the physics, chemistry, sub-surface oil deposition and environmental impacts of deep oil spills. Key lessons learned from the responses to previous deep spills, as well as unresolved scientific questions for additional research are highlighted, all of which are appropriate for governmental regulators, politicians, industry decision-makers, first responders, researchers and students wanting an incisive overview of issues surrounding deep-water oil and gas production.
    Type of Medium: Online Resource
    Pages: XIV, 611 p. 152 illus., 110 illus. in color. , online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030116057
    DDC: 551.48
    Language: English
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  • 3
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    PANGAEA
    In:  Supplement to: Richey, J E; Hollander, David J; Flower, Benjamin P; Eglinton, Timothy Ian (2011): Merging late Holocene molecular organic and foraminiferal-based geochemical records of sea surface temperature in the Gulf of Mexico. Paleoceanography, 26(1), PA1209, https://doi.org/10.1029/2010PA002000
    Publication Date: 2023-05-12
    Description: A molecular organic geochemical proxy (TEX86) for sea surface temperature (SST) is compared with a foraminifera-based SST proxy (Mg/Ca) in a decadal-resolution marine sedimentary record spanning the last 1000 years from the Gulf of Mexico. We assess the relative strengths of the organic and inorganic paleoceanographic techniques for reconstructing high-resolution SST variability during recent climate events, including the Little Ice Age (LIA) and the Medieval Warm Period (MWP). SST estimates based on the molecular organic proxy TEX86 show a similar magnitude and pattern of SST variability to foraminiferal Mg/Ca-SST estimates but with some important differences. For instance, both proxies show a cooling (1°C-2°C) of Gulf of Mexico SSTs during the LIA. During the MWP, however, Mg/Ca-SSTs are similar to near-modern SSTs, while TEX86 indicates SSTs that were cooler than modern. Using the respective SST calibrations for each proxy results in TEX86-SST estimates that are 2°C-4°C warmer than Mg/Ca-SST throughout the 1000 year record. We interpret the TEX86-SST as a summer-weighted SST signal from the upper mixed layer, whereas the Mg/Ca-SST better reflects the mean annual SST. Downcore differences in the SST estimates between the two proxies (DeltaT = TEX86 - Mg/Ca) are interpreted in the context of varying seasonality and/or changing water column temperature gradients.
    Keywords: BC; Box corer; Branched and isoprenoid tetraether index; DEPTH, sediment/rock; PBBC-1; Pigmy Basin; Tetraether index of 86 carbon atoms
    Type: Dataset
    Format: text/tab-separated-values, 174 data points
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  • 4
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    PANGAEA
    In:  Supplement to: Hill, Heather W; Flower, Benjamin P; Quinn, Terrence Michael; Hollander, David J; Guilderson, Thomas P (2006): Laurentide Ice Sheet meltwater and abrupt climate change during the last glaciation. Paleoceanography, 21(1), PA1006, https://doi.org/10.1029/2005PA001186
    Publication Date: 2023-11-04
    Description: A leading hypothesis to explain abrupt climate change during the last glacial cycle calls on fluctuations in the margin of the North American Laurentide Ice Sheet (LIS), which may have routed fresh water between the Gulf of Mexico (GOM) and the North Atlantic, affecting North Atlantic Deep Water variability and regional climate. Paired measurements of d18O and Mg/Ca of foraminiferal calcite from GOM sediments reveal five episodes of LIS meltwater input from 28 to 45 thousand years ago (ka) that do not match the millennial-scale Dansgaard-Oeschger warmings recorded in Greenland ice. We suggest that summer melting of the LIS may occur during Antarctic warming and likely contributed to sea level variability during marine isotope stage 3.
    Keywords: Age, 14C AMS; Age, 14C calibrated; Age, comment; Age, dated; Age, dated standard error; Calendar age; CALYPSO; Calypso Corer; DEPTH, sediment/rock; IMAGES IX - PAGE; Laboratory code/label; Marion Dufresne (1995); MD022551; MD02-2551; MD127; Orca Basin
    Type: Dataset
    Format: text/tab-separated-values, 90 data points
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  • 5
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    PANGAEA
    In:  Supplement to: Lyons, Timothy W; Werne, Josef P; Hollander, David J; Murray, Richard W (2003): Contrasting sulfur geochemistry and Fe/Al and Mo/Al ratios across the last oxic-to-anoxic transition in the Cariaco Basin, Venezuela. Chemical Geology, 195(1-4), 131-157, https://doi.org/10.1016/S0009-2541(02)00392-3
    Publication Date: 2024-01-09
    Description: An abrupt transition from oxic to anoxic-sulfidic (euxinic) marine bottom waters occurred in the Cariaco Basin in response to increasing productivity resulting from the late Pleistocene post-glacial rise in sea level and corresponding increase in surface-water nutrient availability. The microlaminated sediments of the euxinic interval, which span the last not, vert, similar14.5 ky, suggest a predominance of water-column (syngenetic) pyrite formation based on (1) high pyrite sulfur (Spy) concentrations in the surficial sediment layers, (2) values for degree-of-pyritization (DOP) that generally do not increase appreciably with increasing burial, (3) ratios of total iron (FeT) to Al that are elevated above the continental baseline recorded in the underlying oxic sediments, and (4) Spy isotope trends that largely mimic the d34SHS- of the modern water column. Intermediate DOP values in the microlaminated deposits and FeT/Al ratios that are slightly above continental levels indicate an iron reservoir controlled by scavenging during syngenetic pyrite formation in combination with intermediate rates of Fe-bearing siliciclastic accumulation. As predicted from the relative rates of siliciclastic delivery, FeT/Al and DOP data lie between end-member values observed in the modern Black Sea. As viewed broadly, FeT/Al and DOP relationships in euxinic sediments reflect the balance between syngenetic Fe scavenging and temporal and spatial gradients in siliciclastic input. Pyrite concentrations are generally low in the underlying oxic marine deposits because of limitations in the supply of organic carbon (Corg). However, the upper 80 cm of the Fe-rich, Corg-poor, bioturbated sediment show evidence for a strong diffusional HS- overprint from the overlying, Fe-limited euxinic marine environment. This post-glacial transition manifests in pyrite overprints that are strongly 34S-depleted relative to those in restricted, presently euxinic marine settings elsewhere in the world, such as the Black Sea, where the sedimentary sequence spanning the last glacial-interglacial transition begins with a shift from freshwater to Corg-poor oxic marine deposition and thus dominantly sulfate diffusion. Trends for Mo/Al ratios in the microlaminated sediments suggest that Mo is enriched by roughly two orders of magnitude above the continental levels recorded in the oxic deposits. Organic matter plays a role by enhancing HS- production and/or by providing a substrate for Mo scavenging. Significant Mo enrichment via diffusion into the upper portion of the bioturbated zone was not observed despite HS- -rich pore waters as recorded in the heavy iron sulfide overprint. We have not, however, proven that high sulfide concentrations within the water column are required for enhanced Mo sequestration in sediments.
    Keywords: 165-1002; Acidification/coulometry, CaCO3; Aluminium; Calculated; Carbon, inorganic, total; Carbon, organic, total; Cayman Rise, Caribbean Sea; Combustion at 950°C; COMPCORE; Composite Core; DEPTH, sediment/rock; ICP-ES, Inductively coupled plasma - emission spectrometry; Iron; Iron, fractionated; Iron/Aluminium ratio; Joides Resolution; Leg165; Mass spectrometer Finnigan MAT 252; Molybdenum; Molybdenum/Aluminium ratio; Ocean Drilling Program; ODP; Pyritization; Sulfur of pyrite; Wet chemistry; δ34S, FeS2
    Type: Dataset
    Format: text/tab-separated-values, 656 data points
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  • 6
    Publication Date: 2024-02-10
    Keywords: AMAZA_26; AMAZC_O3; AMAZC_RO11B; Amazom_CBM5; Amazom_CBM6; Amazom_CBM7; Amazom_CBM8; Amazon Basin; Amazon River; Amazon Shelf/Fan; BC; BC28; BC44C; BC50; Box corer; Carbon, organic, total; DEPTH, sediment/rock; DEPTH, water; Elevation of event; Event label; extracted from the World Ocean Atlas 2009 (WOA09); Knorr; KNR197-4; KNR197-4-13BC; KNR197-4-13WS; KNR197-4-20BC; KNR197-4-20WS; KNR197-4-21BC; KNR197-4-21WS; KNR197-4-22BC; KNR197-4-22WS; KNR197-4-23BC; KNR197-4-23WS; KNR197-4-25BC; KNR197-4-25WS; KNR197-4-28BC; KNR197-4-28WS; KNR197-4-32bBC; KNR197-4-32bWS; KNR197-4-32cBC; KNR197-4-42bBC; KNR197-4-42bWS; KNR197-4-42dBC; KNR197-4-42dWS; KNR197-4-43BC; KNR197-4-43WS; KNR197-4-44cBC; KNR197-4-49BC; KNR197-4-50BC; KNR197-4-60BC; Latitude of event; Longitude of event; Sample code/label; Sediment sample; SES; Temperature, water; Temperature, water, annual mean; Water sample; WS
    Type: Dataset
    Format: text/tab-separated-values, 162 data points
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  • 7
    Publication Date: 2024-02-10
    Keywords: AMAZA_26; AMAZC_O3; AMAZC_RO11B; Amazom_CBM5; Amazom_CBM6; Amazom_CBM7; Amazom_CBM8; Amazon Basin; Amazon River; Amazon Shelf/Fan; BC; BC28; BC44C; BC50; Box corer; Branched glycerol dialkyl glycerol tetraether, Ia; Branched glycerol dialkyl glycerol tetraether, Ib; Branched glycerol dialkyl glycerol tetraether, Ic; Branched glycerol dialkyl glycerol tetraether, IIa; Branched glycerol dialkyl glycerol tetraether, IIb; Branched glycerol dialkyl glycerol tetraether, IIc; Branched glycerol dialkyl glycerol tetraether, IIIa; Branched glycerol dialkyl glycerol tetraether, IIIb; Branched glycerol dialkyl glycerol tetraether, IIIc; Crenarchaeol; Crenarchaeol isomer; DEPTH, sediment/rock; DEPTH, water; Elevation of event; Event label; Isoprenoid acyclic glycerol dialkyl glycerol tetraether; Isoprenoid dicyclic glycerol dialkyl glycerol tetraether; Isoprenoid monocyclic glycerol dialkyl glycerol tetraether; Isoprenoid tricyclic glycerol dialkyl glycerol tetraether; Knorr; KNR197-4; KNR197-4-13BC; KNR197-4-13WS; KNR197-4-20BC; KNR197-4-20WS; KNR197-4-21BC; KNR197-4-21WS; KNR197-4-22BC; KNR197-4-22WS; KNR197-4-23BC; KNR197-4-23WS; KNR197-4-25BC; KNR197-4-25WS; KNR197-4-28BC; KNR197-4-28WS; KNR197-4-32bBC; KNR197-4-32bWS; KNR197-4-32cBC; KNR197-4-42bBC; KNR197-4-42bWS; KNR197-4-42dBC; KNR197-4-42dWS; KNR197-4-43BC; KNR197-4-43WS; KNR197-4-44cBC; KNR197-4-49BC; KNR197-4-50BC; KNR197-4-60BC; Latitude of event; Longitude of event; Sample code/label; Sediment sample; SES; Water sample; WS
    Type: Dataset
    Format: text/tab-separated-values, 1045 data points
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  • 8
    Publication Date: 2024-02-10
    Keywords: Amazon Shelf/Fan; Branched glycerol dialkyl glycerol tetraether, Ia; Comment; Crenarchaeol; Elevation of event; Event label; Knorr; KNR197-4; KNR197-4-25WS; KNR197-4-28WS; KNR197-4-42dWS; KNR197-4-43WS; Latitude of event; Longitude of event; Sample code/label; Water sample; WS
    Type: Dataset
    Format: text/tab-separated-values, 78 data points
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  • 9
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    PANGAEA
    In:  Supplement to: Zell, Claudia; Kim, Jung-Hyun; Hollander, David J; Lorenzoni, Laura; Baker, Paul A; Silva, Cleverson Guizan; Nittrouer, Charles A; Sinninghe Damsté, Jaap S (2014): Sources and distributions of branched and isoprenoid tetraether lipids on the Amazon shelf and fan: Implications for the use of GDGT-based proxies in marine sediments. Geochimica et Cosmochimica Acta, 139, 293-312, https://doi.org/10.1016/j.gca.2014.04.038
    Publication Date: 2024-02-10
    Description: Branched glycerol dialkyl glycerol tetraethers (brGDGTs) in river fan sediments have been used successfully to reconstruct mean annual air temperature (MAAT) and soil pH of the Congo River drainage basin. However, in a previous study of Amazon deep-sea fan sediments the reconstructed MAATs were ca. 10°C colder than the actual MAAT of the Amazon basin. In this study we investigated this apparent offset, by comparing the concentrations and distributions of brGDGTs in Amazon River suspended particulate matter (SPM) and sediments to those in marine SPM and surface sediments. The riverine brGDGT input was evident from the elevated brGDGT concentrations in marine SPM and surface sediments close to the river mouth. The distributions of brGDGTs in marine SPM and sediments varied widely, but generally showed a higher relative abundance of methylated and cyclic brGDGTs than those in the river. Since this difference in brGDGT distribution was also found in intact polar lipid (IPL)-derived brGDGTs, which were more recently produced, the change in the marine brGDGT distribution was most likely due to marine in-situ production. Consequently, the MAATs calculated based on the Methylation of Branched Tetraethers (MBT) and the Cyclisation of Branched Tetraethers (CBT) were lower and the CBT-derived pH values were higher than those of the Amazon basin. However, SPM and sediments from stations close to the river mouth still showed MBT/CBT values that were similar to those of the river. Therefore, we recommend caution when applying the MBT/CBT proxy, it should only be used in sediment cores that were under high river influence. The influence of riverine derived isoprenoid GDGT (isoGDGT) on the isoGDGT-based TEX86 temperature proxy was also examined in marine SPM and sediments. An input of riverine isoGDGTs from the Amazon River was apparent, but its influence on the marine TEX86 was minor since the TEX86 of SPM in the Amazon River was similar to that in the marine SPM and sediments.
    Keywords: NIOZ_UU; NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University
    Type: Dataset
    Format: application/zip, 3 datasets
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  • 10
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PeerJ 3 (2015): e874, doi:10.7717/peerj.874.
    Description: Research that uses stable isotope analysis often involves a delay between sample collection in the field and laboratory processing, therefore requiring preservation to prevent or reduce tissue degradation and associated isotopic compositions. Although there is a growing literature describing the effects of various preservation techniques, the results are often contextual, unpredictable and vary among taxa, suggesting the need to treat each species individually. We conducted a controlled experiment to test the effects of four preservation methods of muscle tissue from four species of upper trophic-level reef fish collected from the eastern Gulf of Mexico (Red Grouper Epinephelus morio, Gag Mycteroperca microlepis, Scamp Mycteroperca phenax, and Red Snapper Lutjanus campechanus). We used a paired design to measure the effects on isotopic values for carbon and nitrogen after storage using ice, 95% ethanol, and sodium chloride (table salt), against that in a liquid nitrogen control. Mean offsets for both δ13C and δ15N values from controls were lowest for samples preserved on ice, intermediate for those preserved with salt, and highest with ethanol. Within species, both salt and ethanol significantly enriched the δ15N values in nearly all comparisons. Ethanol also had strong effects on the δ13C values in all three groupers. Conversely, for samples preserved on ice, we did not detect a significant offset in either isotopic ratio for any of the focal species. Previous studies have addressed preservation-induced offsets in isotope values using a mass balance correction that accounts for changes in the isotope value to that in the C/N ratio. We tested the application of standard mass balance corrections for isotope values that were significantly affected by the preservation methods and found generally poor agreement between corrected and control values. The poor performance by the correction may have been due to preferential loss of lighter isotopes and corresponding low levels of mass loss with a substantial change in the isotope value of the sample. Regardless of mechanism, it was evident that accounting for offsets caused by different preservation methods was not possible using the standard correction. Caution is warranted when interpreting the results from specimens stored in either ethanol or salt, especially when using those from multiple preservation techniques. We suggest the use of ice as the preferred preservation technique for muscle tissue when conducting stable isotope analysis as it is widely available, inexpensive, easy to transport and did not impart a significant offset in measured isotopic values. Our results provide additional evidence that preservation effects on stable isotope analysis can be highly contextual, thus requiring their effects to be measured and understood for each species and isotopic ratio of interest before addressing research questions.
    Description: Funding was provided by a grant to CD Stallings and TS Switzer from the National Oceanic and Atmospheric Administration, Cooperative Research Program (NA12NMF4540081).
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
    Format: application/vnd.ms-excel
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