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  • 1
    Publication Date: 2011-08-16
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Applied Microbiology; 27; Jan. 197
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  • 2
    Publication Date: 2011-08-17
    Description: An electrochemical method for detecting bacteria, based on a linear relationship between inoculum size and the time of hydrogen evolution, was tested for the early detection and monitoring of coliforms in naturally contaminated estuarine and fresh water samples. Standard methods for coliform analysis were performed on each sample, and membrane filtration counts were used to construct dose-response curves; relationships and results are discussed herein.
    Keywords: MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
    Type: American Water Works Association; vol. 68
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  • 3
    Publication Date: 2011-08-16
    Description: Description of a machine and technique for the automatic inoculation of a plastic tray containing agar media with a culture, using either a conventional inoculation loop or a cotton swab. The design of the machine is simple, it is easy to use, and it relieves the operator from the manual task of streaking cultures. The described technique makes possible the visualization of the overall qualitative and, to some extent, quantitative relationships of various bacterial types in a sample tested.
    Keywords: BIOTECHNOLOGY
    Type: Applied Microbiology; 24; Nov. 197
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  • 4
    Publication Date: 2011-08-16
    Description: An approach involving the measurement of hydrogen evolution by test organisms was used to detect and enumerate various members of the Enterobacteriaceae group. The experimental setup for measuring hydrogen evolution consisted of a test tube containing two electrodes plus broth and organisms. The test tube was kept in a water bath at a temperature of 35 C. It is pointed out that the hydrogen-sensing method, coupled with the pressure transducer technique reported by Wilkins (1974) could be used in various experiments in which gas production by microorganisms is being measured.
    Keywords: BIOSCIENCES
    Type: Applied Microbiology; 27; May 1974
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  • 5
    Publication Date: 2014-09-05
    Description: The light scattering properties of single bacterial cells were examined as a possible means of identification. Three species were studied with streptococcus faecalis exhibiting a unique pattern; the light-scattering traces for staphylococcus aureus and escherichia coli were quite similar although differences existed. Based on preliminary investigations, the light scattering approach appeared promising with additional research needed to include a wide variety of bacterial species, computer capability to handle and analyze data, and expansion of light scattering theory to include bacterial cells.
    Keywords: BIOSCIENCES
    Type: Proc. of the Ann. Conf. of NASA Clinic Directors, Environ. Health Offic. and Med. Program Advisors; p 162-172
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  • 6
    Publication Date: 2011-08-17
    Description: Platinum electrodes with surface area ratios of four to one were used to detect and enumerate a variety of gram-positive and gram-negative organisms. Linear relationships were established between inoculum size and detection time. End points for platinum electrodes were similar to those obtained with a platinum-reference electrode combination. Shape of the overall response curves and length of detection times for gram-positive organisms were markedly different than those for the majority of gram-negative species. Platinum electrodes are better than the platinum-reference electrode combination because of cost, ease of handling, and clearer definition of the end point.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Applied and Environmental Microbiology; 36; Nov. 197
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  • 7
    Publication Date: 2011-08-16
    Description: A simple method for detecting bacteria, based on the time of hydrogen evolution, was developed and tested against various members of the Enterobacteriaceae group. The test system consisted of (1) two electrodes, platinum and a reference electrode, (2) a buffer amplifier, and (3) a strip-chart recorder. Hydrogen evolution was measured by an increase in voltage in the negative (cathodic) direction. A linear relationship was established between inoculum size and the time hydrogen was detected (lag period). Lag times ranged from 1 h for 1 million cells/ml to 7 h for 1 cell/ml. For each 10-fold decrease in inoculum, length of the lag period increased 60 to 70 min. Based on the linear relationship between inoculum and lag period, these results indicate the potential application of the hydrogen-sensing method for rapidly detecting coliforms and other gas-producing microorganisms in a variety of clinical, food, and other samples.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Applied Microbiology; 27; May 1974
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  • 8
    Publication Date: 2011-08-16
    Keywords: BIOTECHNOLOGY
    Type: Applied Microbiology; 27; Mar. 197
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  • 9
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    In:  Other Sources
    Publication Date: 2011-08-16
    Description: An automatic single-slide Gram staining device is described. A timer-actuated solenoid controls the dispensing of gentian violet, Gram iodine solution, decolorizer, and 1% aqueous safranin in proper sequence and for the time required for optimum staining. The amount of stain or reagent delivered is controlled by means of stopcocks below each solenoid. Used stains and reagents can be flushed automatically or manually. Smears Gram stained automatically are equal in quality to those prepared manually. The time to complete one Gram cycle is 4.80 min.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Applied Microbiology; 30; Sept
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  • 10
    Publication Date: 2011-08-16
    Keywords: BIOSCIENCES
    Type: Applied Microbiology; 24; Nov. 197
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