ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Nutrient requirements of callus tissues previously obtained from Vigna sinensis hypocotyl segments were investigated on modified White's (Miller and Skoog) and Murashige and Skoog's media containing 1 mg/1 of each of 2,4-D and kinetin, 2% sucrose and 1% agar. Yields of tissues on the latter medium were 3 times more than the former. After studying various organic and inorganic nutrients, two synthetic media have been developed for callus cultures. The above modified White's medium further supplemented with 2% sucrose, and 500 mg/1 of urea or asparagine was suitable for a compact callus with a dry-to-fresh-weight ratio around 8% and on this the tissues grew 15- to 17-fold in 5 weeks. Similarly the Murashige and Skoog's medium further enriched with 2% sucrose, 400 mg/1 of urea or a mixture of aspartic acid (750 mg/1), glutamic acid (250 mg/1), asparagine (250 mg/1) and arginine (250 mg/1), was apt for a friable callus with a dry to fresh weight ratio of 3 to 4% and it promoted a 45- to 50-fold increase in growth in 5 weeks.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1974.tb03717.x
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