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  • 1
    Electronic Resource
    Electronic Resource
    Influence of Cytokinins and Temperature on Development of Asparagus plumosus Shoot Tips in vitro (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 45 (1979), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Lateral shoot tips from young shoots of Asparagus setaceus (Kunth) Jessop (syn. A. plumosus Baker) were grown on a modified Murashige and Skoog medium. PBA was the most effective cytokinin for growth and development of Asparagus plumosus shoot tips. The optimal range of PBA was between 0.02 and 2 mg/1. Zeatin was less effective, and kinetin, BA and 2iP were poor as cytokinins for A. plumosus. The optimal temperature for growth and development was 21°C. There was interaction between the temperature and the PBA concentration. With increasing temperature from 17 to 24°C the need for PBA increased from 0.2 to 2 mg/1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1979.tb01666.x
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  • 2
    Electronic Resource
    Electronic Resource
    The Influence of NAA, BA and Temperature on Shoot and Root Development from Begonia×cheimantha Petiole Segments Grown in vitro (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 32 (1974), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of exogenously supplied NAA and BA on the shoot and root formation in isolated petiole segments of Begonia×cheimantha was determined in vitro on a modified White medium at a constant temperature of 24°C. The best development of normally appearing plants was obtained on media containing 0.01 mg × 1−1 of NAA and 0.5 to 1.0 mg × 1−1 of BA. Lower concentrations of BA yielded no shoots, higher concentrations promoted shoot formation, but the shoots were abnormal with malformed leaves. Lower concentrations of NAA resulted in poorer survival rate and no roots, with higher concentrations of NAA many roots developed, but these were thickened and their longitudinal growth inhibited. Temperature proved to be of utmost importance for the induction of shoot formation. Thus significantly fewer shoots were formed at the higher temperature (25°C) than at lower temperatures (15 to 20°C). Temperature immediately after initial transfer was of greatest importance: 25°C, during the first week followed by low temperature, produced very few shoots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1974.tb03725.x
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  • 3
    Electronic Resource
    Electronic Resource
    Growth Hormones and Propagation of Cymbidium in vitro (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 27 (1972), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Protocorms of Cymbidium (Orchidaceae) were grown on solid or liquid medium with macro-nutrients according to Wimber (van Raalte 1967) and iron, micro-nutrients and vitamins according to Nitsch (1968) the medium also contained 2% sucrose. The effects of 1) the auxins; indol-3yl-acetic acid (IAA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D); 2) the cytokinins; 6-furfurylaminopurine (kinetin) and benzyladenine (BA) and 3) the gibberellin; gibberellic acid (GA) were examined alone or in combinations.IAA had no effect alone. NAA resulted in optimal fresh weight at 10 μM and the protocorms were vigorous, but lighter green than usual. 2,4-D caused a high weight increase at 1 μM, but the protocorms were abnormal. Higher concentrations of NAA and 2,4-D inhibited chlorophyll synthesis. On solid medium kinetin (100 μM) induced a growth of many small shoots, but had no effect on the fresh weight. In liquid medium, kinetin promoted a callus formation and fresh weight increase. BA had effects similar to kinetin, but at lower concentrations. GA alone promoted shoot and leaf growth.Combinations of kinetin and NAA resulted in a maximal fresh weight increase at kinetin concentrations one tenth of the NAA concentrations. The optimal growth and the best development occurred at 10 μM NAA and 1 μM kinetin. NAA and kinetin together could limit the shoot and leaf growth induced by GA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1972.tb03619.x
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  • 4
    Electronic Resource
    Electronic Resource
    Organic Nutrients in the Media for Propagation of Cymbidium in vitro (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 27 (1972), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Protocorms of Cymbidium (Orchidaceae) were grown on media containing different organic nutrients. Of the sugars tested sucrose was better than maltose, glucose and fructose, and sucrose had an optimum concentration of 3 to 4 %. D-Mannose was significantly less effective than the other sugars. The amino acid mixtures casamino acids (casein hydrolysate) and tryptone increased growth while yeast extract was inhibitory and malt extract without effect. Optimal concentrations were 2 to 3 g · l-1 casamino acids and 3 to 4 g · l-1 tryptone. It was to some extent possible to substitute the amino acid mixtures with a single amino acid (glutamine at 300 mg · l-1). Arginine was inhibitory and asparagine was without any effect. Vitamins proved to be unnecessary although there was a tendency towards increased growth with nicotinic acid and meso-inositol. Purines and pyrimidines were added to the medium but with no effect. Liquid endosperm from coconuts (10 to 15%) increased growth while the liquid endosperm from Aesculus hippocastanum was inhibitory. On the basis of these results a revised medium is proposed for the in vitro propagation of Cymbidium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1972.tb03628.x
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  • 5
    Electronic Resource
    Electronic Resource
    Development of Asparagus plumosus Shoot Tips Grown in vitro (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 40 (1977), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Lateral shoot tips from young Asparagus setaceus (Kunth) Jessop (syn. A. plumosus Baker) shoots were grown on a modified Murashige and Skoog medium. Tips from 5 to 20 mm lateral shoots had significantly better growth and development than tips from lateral shoots (2 mm) still covered by leaf-scale. The optimum temperature for growth and development of the explants was 17 to 24°C. The initial growth was fast at 24°C but stopped after about 4 weeks. At 17°C the growth was slow but in return the cultures continued to grow. Kinetin was necessary for growth. Without any kinetin all cultures died. Optimum growth was found with 2 mg/l kinetin. There was no growth at all with IAA alone. A low IAA concentration had no effect, but at high concentrations IAA inhibited the kinetin induced growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1977.tb01497.x
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  • 6
    Electronic Resource
    Electronic Resource
    Temperature Effects on Shoot and Root Development From Begonia × cheimantha Petiole Segments Grown in vitro (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 32 (1974), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of different temperatures on the shoot and root formation in isolated petiole segments of Begonia × cheimantha was determined after 10 weeks on a modified White medium containing 0.1 mg/1 NAA and 0.5 mg/1 BA.Temperature proved to be important for the induction of shoot and root formation. At a constant temperature the best plants were obtained at 18 to21°C. If the temperature was higher, fewer cultures survived and the number of roots and shoots were lower. Lower temperatures inhibited the development of plants. A pretreatment at 15 or 18°C for two to four weeks improved the number and size of the shoots developed during a following 24°C treatment. High temperatures throughout the growing period reduced the number of shoots severely. A pretreatment of three days at 24°C or one day at 28°C reduced the shoot number by 50 %. After seven days at 28°C there was not a single shoot in any of the cultures. However, after two weeks at 15 or 18°C it was no longer possible to inhibit the shoot formation by a 24°C treatment.It is concluded that the formation of shoots in petiole segments takes place during the first two weeks after excision, and that high temperature is detrimental to the shoot initiation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1974.tb03135.x
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