ISSN:
0741-0581
Keywords:
Residual
;
Asbestos fibers
;
Ferruginous bodies
;
Amosite worker
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
A recurring question that concerns researchers who use digestion techniques for the electron microscopic study of asbestos and ferruginous body (FB) load in tissue is: “Do appreciable numbers of the total fibers or FBs remain adhered to the walls of preparative vials after digestion?”A test series was devised to evaluate the fiber and FB content remaining in digestion vials (glass or plastic) as well as the effectiveness of removing these particulates through variations in the rinsing procedure. The material used for all studies was digested lung tissue from a former amosite asbestos worker. The digestion procedure was based on the bleach technique of Smith and Naylor (Am. J. Clin. Pathol.: 58:250-54, 1972), as modified by Williams et al. (J. Toxicol. Environ. Health:10:627-628, 1982). Aliquots (1.5 ml) of the digests were used to create reproducible standards. These were placed in either the clean glass or polystyrene vials that had been cut to fit in an AMRAY 1000A scanning microscope interfaced with an X-ray energy dispersive analysis system. The aliquots were allowed to remain in the vials for 10 min or 24 h before rinsing was initiated. Aliquots used for the glass vials contained 5.1 × 106 fibers and 20 × 103 FBs, while aliquots for the polystyrene vials contained 6.9 × 106 fibers and 64 × 103 FBs.The maximum errors encountered for the glass and polystyrene vials were, respectively, 0.094% and 0.12% for fibers and 0.0098% and 0.0084% for FBs and are considered small when compared with potential sources for errors in the other stages of the preparative techniques. Also, it was concluded that if at least three thorough rinses are applied, the loss was minimal in either the glass or polystyrene vials.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060060102
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