Publication Date:
2002-08-17
Description:
The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ahlert, Joachim -- Shepard, Erica -- Lomovskaya, Natalia -- Zazopoulos, Emmanuel -- Staffa, Alfredo -- Bachmann, Brian O -- Huang, Kexue -- Fonstein, Leonid -- Czisny, Anne -- Whitwam, Ross E -- Farnet, Chris M -- Thorson, Jon S -- CA08748/CA/NCI NIH HHS/ -- CA84374/CA/NCI NIH HHS/ -- GM58196/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Aug 16;297(5584):1173-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12183629" target="_blank"〉PubMed〈/a〉
Keywords:
*Aminoglycosides
;
Anti-Bacterial Agents/*biosynthesis
;
Antibiotics, Antineoplastic/*biosynthesis
;
Blotting, Southern
;
Chromatography, High Pressure Liquid
;
Cloning, Molecular
;
Conserved Sequence
;
Enediynes
;
*Genes, Bacterial
;
Micromonospora/enzymology/*genetics/metabolism
;
Multienzyme Complexes/*chemistry/*genetics/metabolism
;
Multigene Family
;
Mutation
;
Open Reading Frames
;
Polymerase Chain Reaction
;
Protein Structure, Tertiary
;
Sequence Analysis, DNA
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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